Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"cellular heterogeneity"”.
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Investigating Roles for Cellular Heterogeneity in Cancer
… consideration for the underlying distribution of cellular phenotypes. Though there is growing evidence that variability within cellular populations has some functional consequences, the significance of cell to cell heterogeneity is still poorly understood. Here, we present an analytical platform …
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Gene Regulatory Networks at Single-Cell Resolution: an approach to exploring the impact of genomic regulation on cellular heterogeneity
… data can provide biological insights into cellular heterogeneity because of the single-cell resolution; since scGRNs also contain cell-resolution information, it implies that scGRN can also be analysed about its corresponding cell-related properties. Since scGRN is generated from scRNA-seq …
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Cytometry of Reaction Rate Constant (Crrc): Optimization of the Technique and Development of a Novel Protocol to Assess Cell Population Heterogeneity Based on Aldh1a1 Activity in Vitro
… a novel analytical technique which aims to study cellular heterogeneity based on the activity of enzymatic reactions. In the past, CRRC was able to assess cellular heterogeneity in vitro by investigating the activity of ABC-transporter enzymes. However, CRRC showed poor robustness to highly motile …
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Statistical methods for the integrative analysis of single-cell multi-omics data
… provided an unprecedented opportunity to study cellular heterogeneity at the molecular level. This represents a remarkable advance over traditional bulk sequencing methods, particularly to study lineage diversification and cell fate commitment events in heterogeneous biological processes. While …
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Microfluidics for Cell Culture and Single Cell Assays
… cells individually and facilitate studies into cellular heterogeneity. Existing techniques, however, are often associated with high costs and limited throughput. To address these shortcomings, my PhD has focussed on the methodological advancement of cell culture and assay techniques by taking …
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Functional characterization of mobilized tumor cells
… to enable clonal comparison and mapping of heterogeneity. The viability, invasiveness and secretory profiles of CTCs were measured. Only a subset of CTCs was found to possess malignant traits indicative of metastatic potential. These CTCs were resistant to anoikis, were invasive or secreted …
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Investigating Parkinson’s Disease human iPSC-derived models through single-cell gene expression
… their variability and characterize the heterogeneity of the single-cell states within these cultures. Objective: We use single-cell gene expression data of iPSC-derived DA neuron models to assess their cellular heterogeneity, in vivo biological relevance, and investigate novel use cases …
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Tissue-specific adaptations of cell types
… and organisms in Nature. Across these, common cellular morphologies and functions have been identified. High-throughput, multifactorial profiling of cells has grown exponentially in recent years with the advent of single-cell RNA-sequencing (scRNA-seq), increasingly unravelling cell diversity. …
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Control of flow and oxygen in a 3-D perfused micro-environment fosters balanced survival of hepatocyte-non-parenchymal cell co-cultures
… in vitro liver model requires reproducing the cellular heterogeneity of in vivo liver in a functional state. However differentiated sinusoidal endothelial cells (SECs), marked by SE-1 expression are difficult to maintain in culture while stellate cells easily activate and over-proliferate. We …
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Using single-cell RNA-seq to assess the effect of common genetic variants on gene expression during development
… the single cell level, and the chance to study cellular heterogeneity. This represents a remarkable advance over traditional bulk sequencing methods, particularly to study cell fate commitment events in development. The challenge of studying early human development is partially overcome by …
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An integrated system for selection and manipulation of single cells in culture
Cellular heterogeneity plays a critical role in processes like embryonic development, drug-resistance, and immune response. The ability to select and manipulate individual cells greatly increases the ability to study heterogeneity within cultures. As such, a number of different single cell sorting …
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Cells of the Developing Human Heart and Great Vessels
… has expanded our knowledge of the heart’s cellular heterogeneity. By gaining a deeper understanding of its cellular makeup, we can uncover how these cells converge to define cardiac identity and function and how perturbation to cellular identities can lead to diseases. This dissertation …
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Novel Techniques for Single-cell RNA Sequencing Data Imputation and Clustering
… of individual cells, unveiling the remarkable heterogeneity and complexity of cellular systems. By unraveling the unique signatures and functions of distinct cell types, single-cell technologies have not only deepened our understanding of fundamental biological processes but also unlocked new …
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Combining optical and hydrodynamic forces for single cell characterization, isolation and delivery
With the existence and emerging importance of cellular heterogeneity there has been a push in the microbiology community for new tools to study at the individual cells. To meet the need to identify, characterize and isolate single cells of interest from bulk samples, precise and directed forces on …
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Diurnal and stimulated astrocyte morphology dynamics in the rodent central nervous system
Cellular heterogeneity between brain regions has been attributed to differences in neurons, although differences between astrocytic glia of distinct brain regions are emerging. Relatively little is understood concerning heterogeneity in astrocyte structural dynamics between brain regions. The …
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The chronic response to centrosome loss-induced cell cycle exit
… To examine whether this phenotype is due to cellular heterogeneity or a globally slow-cycling state, I have taken a single-cell transcriptomics approach and found that long-term centrinone treatment results in two distinct sub-populations, one exhibits typical senescence features, the other …
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scPhen: Single-Cell Phenotype Predictor for Alzheimer’s Disease
… provides unprecedented resolution into cellular heterogeneity, offering a powerful substrate for modeling disease circuitry. However, predicting patient-level phenotypes from scRNA-seq remains challenging due to limited sample sizes, variable cell counts, and the computational burden of …
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Development and application of a microfluidic platform for single-cell cultivation of CHO suspension cell lines
… a serious challenge. Due to random mutations, cellular traits like growth behavior, morphology, and productivity can be affected. Besides genetical variations also environmental gradients during cultivation or stochastic biological processes like cell cycle distribution or biochemical …
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Détection, annotation fonctionnelle et régulation des isoformes de l'épissage alternatif associées au cancer de l'ovaire
… these conclusions may be simply explained by the cellular heterogeneity of tumor. In fact, it is well established that alternative splicing is a cell type-specific process. To address this, we microdissected cancer tissues and demonstrated that some splicing isoforms are truly cancer-associated …
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Patient-derived and xenograft-derived organoids for characterization, drugs testing and biobanking of pediatric brain tumors
… models to better understand the complexity and heterogeneity of these tumors and to test personalized treatment strategies remains a clinical challenge. We are trying to answer to this medical need with Patient-Derived Organoids (PDOs) and Patient- Derived Xenograft Organoids (PDXOs) established …
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