Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"cellular fate"”.
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Reprogramming cellular fate using defined factors
Embryonic stem (ES) cells have a vast therapeutic potential given their pluripotency, or the ability to differentiate into tissues from all three germ layers. One of the ultimate goals of regenerative medicine is to isolate pluripotent stem cells from patients. Nuclear reprogramming offers the …
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The Role of Nucleolin Phosphorylation by CK2 in Regulating Cellular Fate Under Normal and Stress Conditions
… ribosome biogenesis, DNA/RNA metabolism, cellular response to DNA damage, cell growth, proliferation and death. In this dissertation, I elucidate the role of nucleolin phosphorylation by casein kinase 2 (CK2) in controlling cellular fate by regulating p53 checkpoint under normal and …
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Enhancement of Dicer as a personalized anticancer strategy
… essential for regulating protein expression and cellular fate. Misregulation of miRNA production is emerging as a key factor in the development of many disease states, including most forms cancer. A global downregulation of miRNA processing is common among cancers and has been shown to drive …
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PROBING CELLULAR MECHANO-SENSITIVITY USING BIOMEMBRANE-MIMICKING CELL SUBSTRATES OF ADJUSTABLE STIFFNESS
… play a pivotal role in the regulation of cellular fate and function. However, the underlying mechanisms of cellular mechanosensing still remain a topic of open debate. Traditionally, advancements in this field have been made using polymeric substrates of adjustable stiffness with …
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Molecular evolution of the p53 network in reptiles
… to DNA-damage by several options: apoptosis, cellular senescence, and temporary arrest of cellular growth for DNA repair. The p53 network's tight regulation of cellular fate after damage has obvious beneficial effects of preventing tumorigenesis, and possible costly effects later in life such …
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COMBINATORIAL DEVELOPMENT OF NANOSTRUCTURED MATERIAL LIBRARIES FOR THE STUDY OF CELL SUBSTRATE INTERACTION
An in vivo cellular microenvironment in which cells are immobilized contribute an essential role in diverse cellular behavior, consisting of cellular morphology, dynamics and eventually cellular fate. Micro and nanofabrication tools widely used to mimic the in vivo surrounding to manipulate …
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Modularity in Somatic Cell Reprogramming
Reprogramming relies on the concept that cellular fate is plastic, and therefore a considerable effort has been made to develop molecular strategies that reset the epigenome to achieve distinct cell fates, including stem cells. Deriving induced pluripotent stem (iPS) cells has thus emerged as a …
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Role of DNA replication timing in gene expression and chromatin organisation
… timing (RT) is a cause or a consequence of cellular fate changes. During my thesis I used a conditional system to perturb DNA RT in a single cell cycle in budding yeast, in combination with whole-genome sequencing techniques such as replication profiles, RNA-Seq and MNase-Seq in order to …
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Tissue-Specific Matrix Control of Cell Cohesion and Migration Signaling Complexes
<p>The extracellular matrix (ECM) is a complex, interconnected network of three major constituents: collagens, glycoproteins, and proteoglycans, along with their enzyme modifiers. Within this network and beyond the structural role, each ECM molecule contributes a context-specific signal that …
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Probing cellular mechano-sensitivity using biomembrane-mimicking cell substrates of adjustable stiffness
… play a pivotal role in the regulation of cellular fate and function. However, the underlying mechanisms of cellular mechanosensing still remain a topic of open debate. Traditionally, advancements in this field have been made using polymeric substrates of adjustable stiffness with …
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Development of High-Throughput Methods for Screening the Non-coding Genome
<p>Cellular fate specifications and functions are controlled through precise and complex patterns in gene expression. Although all cells in the human body share the same DNA sequence, the expression of RNA and proteins is diverse across tissue and cell types. This orchestrated control of thousands …
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Biophysical and Therapeutic Approaches to Neurodegenerative Disease: Insights From Lysosomal and RNA-Binding Protein Systems
… diseases arise from progressive failures in the cellular systems that maintain protein and RNA homeostasis, yet how distinct molecular defects contribute to the disease pathogenesis is incompletely understood. This dissertation investigates two complementary arms of this network: lysosomal …
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The Modulation of Life and Death: Small Molecule Activators and Inhibitors of Necrotic and Apoptotic Pathways
The proper modulation of an individual cell's fate is critical to multi-cellular organisms. Manipulation of cellular fate through the use of small molecules can be instrumental in studying a cell's death pathways and for treating various pathologies. Therefore, the overarching theme that …
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Modulating ADAM-10 activity and expression in cervical and oesophageal cancer cells
… transmembrane and secreted proteins essential in cellular fate determination, wound healing, cell migration, proliferation and angiogenesis. Previous studies have linked a range of ADAMs, which include ADAM10 to cancer development and progression. Research in our laboratory found endogenous ADAM10 …
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O-GlcNAcylation: A key regulator of hepatic proliferation and differentiation
… O-GlcNAcylation plays a role in regulating the cellular fate of various cell types, including hematopoietic stem cells and preadipocytes. In these studies, we demonstrated that O-GlcNAcylation regulates hepatic differentiation and liver regeneration and protects against the development of …
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Identification and characterization of novel autoregulatory mechanism controlling ataxia telangiectasis mutated gene expression, protein traffiking and function
… determine the process of decision-making of cell fate and involves the participation of multiple proteins. This vital protein acts first by sensing double stranded DNA breaks and second by transducing the signal and activating other downstream proteins of the repair pathway via its kinase …
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Biophysical Studies of Protein Assemblies
… subtle balance of their interactions determines cellular fate at the molecular level. Characterisation of the model under simple biochemical conditions revealed large differences in affinities among binary interactions; the consequence of the lifetime of the complexes, not their speed of …
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Impact of mechanical signals on human foetal lung cell differentiation
… tissue stiffness can play a role in defining cellular fate. I measured the stiffness of human embryonic lung slices over developmental time using Atomic Force microscopy-based indentation measurements and found both temporal and spatial stiffness gradients. The spatial gradient revealed that …
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Recall Germinal Center and Antibody Responses
… employing various prime-boost models in genetic fate-mapping mice, address multiple aspects of the recall B cell and antibody response. First, using cellular fate-mapping in which we mark the primary-cohort B cells and their MBC progeny with fluorescent proteins, we addressed the contribution of …