Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"cellular engineering"”.
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Functional "backpacks" for cellular engineering
… new functions without impairing native cellular behaviors. The first part of this thesis focuses on the techniques to create a heterostructured backpack. Photolithographic methods were developed to geometrically pattern multilayer films into a desired size and shape. A host of polymer …
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Quantitative methods for multiplexed cellular engineering and directed evolution
… through pooled libraries has gained traction in engineering biological behavior. In particular, it has been effective at controlling cells, designing proteins, determining targets for gene therapy, and developing small molecule drugs. This thesis contributes to cellular engineering and …
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Applications of forward genetic screens to LncRNAs, cancer immunotherapy, and cellular engineering
… activation screening to gain insight into the cellular processes that govern tumor resistance to immunotherapy. We identified four candidate genes in our screen, which we validated in diverse cancer cell types and explored through mechanistic studies, leading to the discovery of novel …
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Elucidation of an oral drug delivery mechanism to the mammalian gastrointestinal tract via an engineered T4 phage
… explored as vectors for biologic delivery, yet cellular engineering approaches have traditionally lowered fitness and colonization potential of these microbial factories. As a result, recombinant bacteria are often outcompeted by the native gut flora, requiring high concentrations to alleviate …
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Controlled Myogenic Differentiation of Mammalian Cell Lines by Temperature-Inducible Systems
… for precise regulation of gene expression and cellular responses, leading to rapid and efficient production of mature muscle fibers without the need for expensive media formulations or genetic-based drug-inducible systems. This temperature-dependent cellular engineering method offers …
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Systematically and proactively testing variant effects for AIRE and SOD1
… have recently been made possible by advances in cellular engineering and next-generation sequencing. Variant effect maps present proactive functional evidence for nearly all possible rare variants– even for those that have not yet been seen in the clinic. I led the generation, validation, …
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Forward and inverse metabolic engineering strategies for improving polyhydroxybyrate production
Forward metabolic engineering (FME) is a rational approach to cellular engineering, relying on an understanding of the entire metabolic network to direct perturbations for phenotype improvement. Conversely, inverse metabolic engineering (IME) uses a global, combinatorial approach to identify …
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Somatic cell gene therapy for diabetes mellitus: engineering a surrogate B-cell
… evidence that it is possible to construct, by cellular engineering, a glucose-sensitive insulin-secreting surrogate β-cell. Therefore, somatic cell gene therapy offers a feasible alternative for insulin delivery in IDDM patients.
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Engineering Myeloid Cell Phenotype Using Cell Surface-Adhered Microparticles for Therapeutic Applications
… cell types, and provide a canvas for further cellular engineering. The crucial role of the innate immune system, and particularly myeloid cells, in the dysregulated biological processes in numerous diseases has come into focus, motivating the development of myeloid cell therapies. The …
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Design and engineering of multi-step (bio)catalytic systems
… With the development of protein and metabolic engineering tools and strategies, more and more enzymes have been used in the industries to improve the chemical processing; and microbes such as Escherichia coli and Saccharomyces cerevisiae have been engineered to produce a wide variety of …
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Recombinant Engineering Strategies for the Production of Therapeutic Coagulation Factor Proteins
… strategy. To expand the utility of our engineering approach we then replicated our engineering approach in a murine cell line, programmed it for expression of a murine specific coagulation factor protein, and validated subsequent gene overexpression. Our collective efforts establish …
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Kir-Based Inhibitory Cars Overcome Car-Nk Cell Trogocytosis-Mediated Fratricide and Tumor Escape
<p>Trogocytosis is an active process that transfers surface material from targeted to effector cells. Using multiple in vivo tumor models and clinical data, we report that chimeric antigen receptor (CAR) activation in natural killer (NK) cells promoted the transfer of the CAR-cognate-antigen from …