Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 23 for “"cell-type specificity"”.
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Cell-type specificity and herbivore-induced responses of primary and terpene secondary metabolism in Arabidopsis roots
… constitutive defenses to specific tissues or cells. In this study, we investigated aspects of the spatial regulation and induced changes of primary and secondary metabolism in roots of Arabidopsis thaliana. Roots represent important organs for anchoring plants in the soil and taking up water …
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The circadian role of Pex5 in glial cells and implications of Pex5 in sleep behavior and brain lipidome
Peroxisomes are critical organelles that detoxify cellular waste while also catabolizing and anabolizing lipids. How peroxisomes coordinate protein import and support metabolic functions across complex tissues and timescales remains poorly understood in vivo. Using the Drosophila brain, we discover …
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Opto-mechanical control of nerve growth
… to control neurite growth that promises greater cell-type specificity than commonly used electrical stimulation. Using dorsal root ganglia (DRGs) expressing channelrhodopsin 2 (ChR2) as a test system, I have investigated a broad range of optical stimulation parameters and identified conditions …
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U0126, MEK1/2 inhibitor, Induces Adhesion Molecules Expression in Human Blood Cells
… transmigration and adherence to endothelial cells, causing inflammatory responses in vascular lesion. MAPKinase pathway has been known to be activated in inflammatory responses. In this study, we investigated the expression of adhesion molecule by MEK1/2 inhibotors such as U0126 in human …
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Machine Learning Methods for High Throughput Biological Data
… to derive an effective classifier of cell-type specificity. In my second project, I consider the problem of predicting mass spectra of small molecules: previous methods suffer from a tradeoff between capturing high-resolution mass information and a tractable learning problem, which I …
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Use of gene expression to characterize heterogeneous liver cell populations
Non-parenchymal cells (NPC's) are integral to recreate the native hepatic microenvironment and necessary to maintain in vivo liver function. A variety of in vitro culture systems have been developed to address different aspects of liver physiology and architecture in order to recreate the …
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Muscle Recruitment Mechanism under Optogenetic Neuromodulation
… physiological recruitment mechanism. FES has low-specificity, making neighboring tissues susceptible to simultaneous activation. These drawbacks make force modulation difficult, limiting its controllability. Recently, functional optogenetic stimulation (FOS) has demonstrated cell-type specificity …
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Profiling neuroinflammation in amyotrophic lateral sclerosis
… brain regions of deeply clinically phenotyped C9-ALS-FTSD post-mortem cases (n = 10), compared with age- and sex-matched controls (n = 10). Guided by the results of this analysis, C9-ALS-FTSD post-mortem motor cortex tissue was further profiled using a NanoString nCounter …
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The Contribution of Retrotransposons to the Transcriptomes of Murine Somatic Cells
… retrotransposon transcription in adult somatic cells. Retrotransposons are also responsible for generating gene copies in mammalian genomes (retrocopies), and there are several examples of retrocopies evolving into new genes, or being transcribed as non-coding RNA. Using computational …
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Role of the IKKβ/NF-κB pathway in alcoholism
… for innate- and adaptive-immune responses, cell proliferation/death, and inflammation. More specifically, the NF-κB canonical pathway is responsible for the expression of pro-inflammatory genes. The inhibitory kappa-B kinase (IKK) complex, composed of IKKα, IKKβ, and IKKγ represents a point …
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An investigation into the molecular mechanisms underlying retinitis pigmentosa 17 with the view to developing novel gene- based therapies
… the molecular mechanisms underlying RP17, three cell types were transfected with the wild type (WT ) and the R14W mutant form of the protein. We show using immunocytochemistry that unlike transfected WT CAIV which is transported to the plasma membrane of transfected COS-7 and HT-1080 cells, R14W …
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Transcriptional Heterogeneity in ALS: Patients with Retrovirus-mediated Disease and Potential Cell-type Specific Therapeutic Approaches
… biological pathways converging on a similar phenotype. To address this hypothesis, a bulk RNA sequencing (RNA-seq) dataset from almost 2,000 ALS and unaffected control samples was leveraged to uncover transcriptionally distinct patient subpopulations. Using non-negative matrix factorization (NMF), …
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Deep Molecular Characterization of Human Cortical Cell-Types
… functionally, and molecularly distinct cell-types, the neocortex is organized into cytoarchitectonically and functionally diverse regions. In concert, the diverse cell-types that are found within the diverse cortical regions build the center of all higher cognitive functions, including …
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Sodium dichloroacetate (DCA) impairs β-secretase-mediated proteolysis of the alzheimer's disease amyloid precursor protein (APP)
… we show that, in human neuroblastoma SH-SY5Y cells, the mitochondrial modulator dichloroacetate (DCA) impairs the β-secretase-mediated processing of APP as determined by impaired sAPPβ production. Conversely, the non-amyloidogenic processing of APP was enhanced resulting in more sAPPα …
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Tissue-specific interactions between oncogenic K-ras and the p19A̳r̳f̳_p53 pathway determine susceptibility to transformation
… engage tumor suppressor programs, such that cells harboring oncogenic lesions frequently must acquire secondary mutations that disable these anti-proliferative responses before progressing to overt transformation. This tight coupling represents a critical checkpoint protecting against tumor …
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Molecular mechanism of glycogen phosphorylase gene regulation during Dictyostelium development
Development of multicellular organisms is one of the most fundamental but least understood biological processes. Due to its simple life cycle, the lower eukaryote Dictyostelium has been used as a model system to study several basic biological problems, such as cell differentiation, cell motility, …
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Uncovering Regulators of Whole-Body Metabolism by Chemoproteomic Profiling of the Adipocyte Secretome
… complications such as cardiovascular disease, type 2 diabetes, hypertension, and many types of cancers. Whereas white adipocytes efficiently store energy in the form of triglycerides, thermogenic brown and beige adipocytes can dissipate energy into heat. Adipose tissue is now considered an …
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THE INTERACTION OF TRANSCRIPTION FACTORS WITH THE LONG CONTROL REGION OF HPV 16
Human papillomavirus type 16 (HPV 16) infects mucosal epithelia and causes neoplastic lesions that may progress towards cancer. A segment of 850 base pairs (bp) of the 7904 bp circular DNA genome of HPV 16 termed the long control region (LCR) does not contain genes but contains the control elements …
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REGULATION OF THE HUMAN NEUROTROPIC POLYOMAVIRUS, JCV, IN THE CENTRAL NERVOUS SYSTEM
… Productive infection of JCV occurs only in glial cells and this restriction is to a great extent due to the activation of the viral promoter that has cell type-specific characteristics. The cell types that support the JCV infection cycle in culture are limited to primary human fetal glial cells …
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The Regulation of Effort Exertion by the Nucleus Accumbens Core
… accumbens. In the accumbens there are two main types of neurons, the direct and indirect pathway neurons, that play different roles in behavior and project to different downstream structures. The accumbens has also traditionally been divided into two subregions, the core and the shell. Much of …
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