Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"cell-to-cell variability"”.
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Origins of cell-to-cell variability in apoptosis
… a population of organisms is typically ascribed to genetic differences. However, even members of a genetically identical group of cells or organisms in identical environments can exhibit variability in state and phenotype. One striking example of such heterogeneity is revealed when a genetically …
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RabAnalyser: a computational framework to explore the cell-to-cell variability of Rab GTPases organization
L'abstract è presente nell'allegato / the abstract is in the attachment
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
Cell-to-cell variability in clonal populations is reflected in a distribution of mRNA and protein levels among individual cells, including those of key transcription factors governing embryonic stem cell (ESC) pluripotency and differentiation. This may be a source of heterogeneity resulting in …
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Oncogene-induced remodelling of cellular networks
Biochemical networks maintain cellular homeostasis by processing information from the intra- and extra-cellular environments and coordinating appropriate responses to stimuli and stressors. During carcinogenesis, cells accrue genetic alterations that rewire signalling pathways, eventually leading …
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New experimental approaches to the population balance equation:Eulerian and Lagrangian viewpoints
Cell-to-cell variability in an asynchronous population of cells can be generally related to genetic differences, to different positions in the cell cycle, to the exposure of a heterogeneous environment, or to stochastic variations due to the low number of molecules in individual cells. To …
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Simultaneous SNV calling and Phylogenetic Inference for Single-cell Sequencing Data
Single-cell sequencing provides a powerful approach for elucidating intratumor heterogeneity by resolving cell-to-cell variability. However, it also poses additional challenges including elevated error rates, allelic dropout, and non-uniform coverage. Variant calling in this context is the task of …
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Measuring compositional and growth properties of single cells
The physical properties of a cell are manifestations of its most basic molecular and metabolic processes. In particular, size has been a sought metric, which can be difficult to ascertain with great resolution or for smaller organisms. The advancement of single-cell measurement techniques and the …
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Developing biophysical markers for anemic disorders through advancing interferometric microscopy
Erythrocytes, better known as red blood cells, among various functions, are mainly tasked with the oxygen transport in vertebrates through blood circulation. Red blood cells are packed with the hemoglobin, an oxygen-binding molecule, and have unique biophysical properties that are critical in …
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Mechanisms underlying cell-to-cell diversity in clonal populations of yeast
… their association with downstream DNA binding factors and chromatin remodelers. The genes regulated by these promoters encode cell-surface glycoproteins that mediate a range of cell-to-cell and cell-to-surface adhesions. Phenotypic diversity in clonal populations of yeast cells is mediated in part …
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Analysis of robustness and stochasticity in biochemical networks
Cells are constantly faced with the challenge of functioning reliably while being subject to unpredictable changes from within and outside. Here, I present two studies in which I analyze how biochemical circuits that regulate signaling and gene expression can generate robustness or phenotypic …
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Optimisation and validation of tools for in situ visualisation of heteroplasmic mtDNA variants.
Mitochondria is more than just a powerhouse in our cells; it holds its own genome (mtDNA). Eukaryotic cells often contain many hundreds to thousands of copies of mtDNA. When a mutation occurs in one or more of these copies, this results in a mixture of mutant and wildtype mtDNA, known as …
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On connections between metazoan cellular metabolism and cell size
All animal cells maintain cell size homeostasis, where cell growth (increase in size) is balanced with proliferation (reduction in size via cell division). Yet, different cell types have different sizes and there are physiologically relevant situations where animal cells undergo major cell size …
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Oncogenic KRAS drives heterogeneity in metabolic and signalling pathways through genetic and non-genetic mechanisms
… event is often an activating mutation in key cell signalling hub KRAS. Despite extensive research into PDAC and KRAS, the impact of both genetic and non-genetic variability during the earliest steps of carcinogenesis are scantly described. We hypothesise that both forms of heterogeneity might …
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Microfluidics and live imaging advances: applications in host/pathogen, immunity and stem cell single cell phenotyping
Live single-cell imaging has emerged as an advanced single-cell study tool for approaching a quantitative understanding of many biological questions in recent years. In previous cell studies using bulk cell measurements, the population averages can miss the information from cell to cell variability …