Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"cell-free-system"”.
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eIF4E Phosphorylation Balances Cap-dependent and Cap-independent Translation Initiation
… eIF4E activity remains incomplete. We employ a cell-free system to demonstrate that eIF4E function is required for efficient cap-dependent translation but inhibitory for translation of both cellular and viral RNAs relying on cap-independent mechanisms. Furthermore, we show that phosphorylation …
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Identifying a role for Ciz1 in the DNA damage response
In proliferating cells, cell cycle arrest and DNA repair are induced upon detection of DNA damage. The DNA damage response (DDR) to double strand DNA breaks is mediated by phosphatidylinositol 3-kinase-like protein kinases (PIKKs), which propagate DDR signalling by phosphorylating SQ/TQ motifs in …
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Olfactory-related receptors : methods towards enabling structural and functional studies
… endogenously or in heterologous expression systems. Second, detergents capable of solubilizing and stabilizing these proteins in aqueous solution must be found. Third, the flexible nature of GPCRs can inhibit crystal lattice formation. Methods for overcoming each obstacle were developed. …
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Gene Expression in Rhodopseudomonas Sphaeroides (Photosynthesis, Carboxylase, Membrane Biosynthesis
A DNA-directed coupled transcription-translation system has been developed in cell-free extracts from the facultative phototroph, Rhodopseudomonas sphaeroides. The in vitro protein synthesizing system was active when prepared from either aerobically or anaerobically grown cells. Optimal activity …
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Molecular Mechanisms of Inward and Outward Budding From Multivesicular Endosomes
… transporters, ion channels, receptors) on the cell surface can modify their response to extracellular cues and allow for cellular adaptation to environmental conditions. The fate of membrane proteins that are internalized from the plasma membrane and arrive at the limiting membrane of the late …
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ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙ ΠΟΝΤΙΚΟΥ C3HB
A CELL FREE SYSTEM OF PROTEIN SYNTHESIS OF MOUSE LIVER WAS DEVELOPED. A MOST INTERESTING FEATURE OF THIS SYSTEM IS ITS VERY HIGH ACTIVITY WITH ENDOGENOUS MRNA. A WIDE RANGE OF PRODUCTS ARE LABELLED DURING OPERATION OF THE SYSTEM. THERE IS A LAG PERIOD IN THE LABELLING OF SMALL AND HIGH MOLECULAR …
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Key Role of Lys63-Linked Polyubiquitination in Viral Activation of IRF3
… and proliferation are detected by mammalian cells through receptors belonging to pattern-recognition receptors family (PRRs). Among PRRs, RIG-I-like receptors (RLRs), including RIG-I, MDA5 and LGP2, are responsible for sensing intracellular viral RNAs. MAVS, a mitochondria-localized …
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ΦΩΣΦΟΡΥΛΙΩΣΗ ΠΡΩΤΕΙΝΩΝ ΣΕ ΣΥΣΤΗΜΑ ΠΡΩΤΕΙΝΟΣΥΝΘΕΣΗΣ ΕΛΕΥΘΕΡΟ ΚΥΤΤΑΡΩΝ ΑΠΟ ΣΥΚΩΤΙΠΟΝΤΙΚΟΥ. ΚΑΘΑΡΙΣΜΟΣ ΚΑΙ ΧΑΡΑΚΤΗΡΙΣΜΟΣ ΜΙΑΣ ΝΕΑΣ ΚΙΝΑΣΗΣ ΠΡΩΤΕΙΝΩΝ ΠΟΥ ΡΥΘΜΙΖΕΤΑΙ ΑΠΟ ΚΥΚΛΙΚΑ ΝΟΥΚΛΕΟΤΙΔΙΑ
… PHOSPHORYLATION OF THE PROTEIN COMPONENTS OF A CELL FREE SYSTEM OF PROTEIN SYNTHESIS OF MOUSE LIVER (POLYSOMES,"PH5" FRACTION) AS WELL AS THE EFFECT OF VARIOUS AGENTS ON PROTEIN PHOSPHORYLATION IS REPORTED. THE ENZYME IS A SERINE- THREONINE KINASE WITH A MOLECULAR MASS OF 90 KDA. IT CONTAINS A …
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The genome-wide analysis of the specification and activation of human chromosomal DNA replication origin sites
Background: Human DNA replication is a critical cellular process that initiates at tens of thousands of DNA replication origins. Next-generation sequencing (NGS) enabled the elucidation of some characteristics that define human replication origins. Initiation-site sequencing (iniSeq) utilises the …
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Molecular Study of the B19 (Human) Pathogenic Parvovirus
… B19 is specific for erythroid progenitor cells and has been propagated in vitro only with human erythroid bone marrow. Replication of viral DNA and the viral protein products of B19 appear similar to those of other animal parvoviruses. However, B19 differs from other parvoviruses in some …
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Mechanisms and Regulation of Organelle Transport in Xenopus Melanophores
Organelle transport in eukaryotic cells is a process essential to many cellular functions. Understanding the mechanisms that drive and regulate these movements is, therefore, an important goal for cell biology. We have addressed these issues by studying pigment granule motility in Xenopus …
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ISOLATION AND CHARACTERIZATION OF ARGINASE ISOZYMES FROM BOVINE LIVER AND BRAIN
… enriched mRNA preparation was translated in a cell-free system. After immunoprecipitation and SDS electrophoresis, the translated products migrated to the same positions as authentic liver arginase isozymes.</p>
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Stenodactilina e lanceolina: due nuove potenti “ribosome-inactivating proteins” bicatenarie. Purificazione, caratterizzazione chimico-fisica e studio dei meccanismi citotossici
… These two lectins agglutinate red blood cells, inhibit protein synthesis in a cell-free system as well as in whole cells, and depurinate ribosomes and DNA, but not tRNA or poly(A). They are highly toxic to cells, in which they induce apoptosis and strongly inhibit protein synthesis, and …
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Parallel effects of signal peptide hydrophobic core modifications on cotranslational translocation and posttranslational cleavage by purified signal peptidase
… is not well defined, particularly in mammalian systems, its length and hydrophobicity have been shown to be important characteristics. To determine the requirements for length and hydrophobicity of a mammalian hydrophobic core, amino acids were substituted and deleted from this region of the PTH …
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Studies on Chloroplast Protein Synthesis In Vivo and In Vitro
… were synthesized inside the chloroplast. Cells were labeled in vivo with [<sup>35</sup>S]-sulfate in the presence of either. chloroplast or cytoplasmic protein synthesis inhibitors, and fractionated into soluble proteins, thylakoid membranes, and chloroplast ribosomal subunits. The use of …
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A Transmembrane Precursor of Secretory Component
… secretions, is produced by certain epithelial cells and is thought to be the receptor mediating the transcellular transport of pIg. We studied the biosynthesis and processing of rabbit and human SC. Using translation of mRNA from rabbit mammary gland and liver in a cell-free system supplemented …
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Studies of G-Protein Coupled Receptors Incorporated Into a Novel, Nanoscale, Membrane-Mimetic System
… optimized a heterologous bacterial expression system, and protein purification and labeling scheme for obtaining high-yields of pure zebrafish apo A-I. We demonstrated that zebrafish apo A-I forms stable, homogeneous discoidal lipoprotein particles, which we termed as Nanoscale Apolipoprotein …
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Molecular Cloning of the cDNA for Porcine Parotid Hormone
… of porcine tissues indicated that the acinar cells of the parotid glands are the primary location for both parotid hormone related mRNAs and the translation products.</p> <p>The expression of cloned parotid hormone containing cDNAs produced a single protein in the cell-free system and multiple …
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Antibiotics and human phagocytic cells
… of this thesis concerned human phagocytic cells in vivo as they responded to physical trauma in an experimental skin abrasion. This showed the sequence of cellular responses during acute inflammation and provided quantitative data about the phagocytic cell concentrations at each stage. 2. …
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Micro-analysis of adenylate cyclase and cyclic AMP-dependent protein kinase activities in human mammary tumors
… and type(s) of regulation of these enzymes in a cell-free system. Adenylate cyclase (AC) activity levels were higher in benign than malignant tumor tissue on a dry wt basis. A 2 to 3-fold activation by Gpp(NH)p and a 4 to 5-fold stimulation by fluoride was demonstrated. Calmodulin activation of …
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