Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"cell-free systems"”.
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Insertion Signal and Membrane Anchor Functions of the Amino Terminus of Cytochrome P45011C2
Proteins destined for export from a cell are synthesized in a larger precursor form with the cleaved pre sequence acting as the signal for translocation across the endoplasmic reticulum. Proteins integrated into the endoplasmic reticulum also possess translocation signals, but such signals are not …
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Methyl esterification of fatty acids and eicosanoids with a novel reagent trimethylsilyldiazomethane for analysis by gas chromatography-mass spectrometry
… be used to characterize by GC-MS these lipids in cell culture and in other cell-free systems, such as those containing enzymes like COX.</p>
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Regulation and role of the LKB1-AMPK pathway
… protein kinase (AMPK) is a sensor of cellular energy that is activated by increases in the intracellular AMP:ATP or ADP:ATP ratios. Once active, AMPK activates catabolic pathways and inhibits anabolic pathways to restore cellular energy homeostasis. AMPK activity can be increased by …
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Towards The Engineering Of A Synthetic Minimal Cell
Cell-free expression (CFE) systems are one of the main platforms for building a synthetic cell. A major drawback is the orthogonality of cell-free systems across species. To generate a CFE system compatible with recently established minimal cell constructs, we attempted to optimize a Mycoplasma …
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Early Events in the Biosynthesis and Subcellular Consignment of Secretory and Membrane Proteins
… in light of the signal hypothesis. Model systems for the study of the biosynthesis of these different classes of proteins, as well as for the study of specific events common to both, have been developed. Their common as well as distinctive features have been probed by a variety of …
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Programmable Microparticle Scaffolds for Enhanced Diagnostic Devices
… particularly difficult. However, biological systems demonstrate the key features of robotics " sensing, actuation, processing" and are remarkably complex at the microscale. As such, many researchers have turned to biology for inspiration and living robotic components. In our laboratory we …
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Characterization of Selective Inhibition of Adenylyl Cyclase Activity By Small Molecule Inhibitor Nky80
… Due to this mechanism and nanomolar potency in cell-free systems, attempts at ligand-based drug design of novel AC inhibitors frequently use P-site inhibitors as a starting template. One small molecule inhibitor designed through this process, NKY80, is described as an AC5 selective inhibitor …
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Interplay Between Estrogen Response Element Sequences and Ligands Controls Binding of Estrogen Receptor to Regulated Genes
… gene expression, I established both in vitro and cell-based systems. To study estrogen regulation of transcription in vitro, I developed the CHO-S system for expression of ER. Appropriate concentrations of CHO-S-expressed ER activated transcription in cell-free systems and bound DNA in …
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Synthetic enzymatic pathway conversion of cellulosic biomass to hydrogen
… production of hydrogen from biomass sugars. Lignocellulosic biomass, which includes agricultural residues, energy crops, and industrial waste streams, is an ideal substrate for SyPaB conversion, as it is abundant and cheap, second only to untaxed coal on a $/energy content basis. But the structure …
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Thermodynamics of λ-PCR Primer Design and Effective Ribosome Binding Sites
… of the protein was measured using both whole-cell and cell-free systems to determine which system yields higher protein concentrations. A number of other factors were tested to optimize conditions for high protein expression, including: induction time, IPTG concentration, temperature, and …
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Studies of viral and cellular proteins involved in herpes simplex virus type-1 egress
… a guided pathway that is directed towards the cell adherent points for secretion. Once secreted the vast majority of virions remain associated with the extracellular membrane of cells and very few free virions are released into the culture medium (<1%). The mechanisms that mediate both the …
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The role of the herpes simplex virus type 1 UL33 protein in DNA packaging
… Immunofluorescence studies of virally infected cells revealed that UL15 was necessary for the localisation of the remaining terminase components (UL28 and UL33) to nuclear sites of viral DNA replication, where packaging occurs. This is consistent with a model originally proposed by Yang et al. …
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Toca-1 driven actin polymerisation at membranes
Regulation of the actin cytoskeleton is key to cellular function and underlies processes including cell migration, mitosis and endocytosis. Motile cells send out dynamic actin protrusions that enable them to sense and interact with their environment, as well as generating physical forces. Linking …
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Stochastic Geometry Perspective of Massive MIMO Systems
… fifth-generation (5G) and beyond communication systems. For successful integration of this promising physical layer technique in the upcoming cellular standards, it is essential to have a comprehensive understanding of its network-level performance. Over the last decade, stochastic geometry has …
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NOVEL MICROTUBULE TARGETING AGENTS: SYNTHESIS AND STRUCTURAL DETERMINATION OF THEIR INTERACTIONS
… how they affect the behaviour of microtubules in cell free systems. The chosen compounds are further studied in neuronal models, in order to clarify how small molecules affect microtubule dynamics in neurons. In Chapter I, the introduction on microtubules, their structure, and functions, and in …