Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"cell-free protein synthesis"”.
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The Democratization and Development of Cell-Free Protein Synthesis
<p>Cell-free protein synthesis (CFPS) using crude lysates has developed into a robust platform technology over the last 60 years to express numerous types of recombinant proteins. The open-nature, elimination of reliance on cell viability, and focus of all energy towards production of the protein …
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Translational Incorporation of Unsaturated Amino Acids
In nature proteins are synthesised using twenty standard proteinogenic amino acids. The unique linear sequences of these monomeric building blocks dictate the structure and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due …
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Translational Incorporation of Unsaturated Amino Acids
In nature proteins are synthesised using twenty standard proteinogenic amino acids. The unique linear sequences of these monomeric building blocks dictate the structure and functionality of the translated protein. Misincorporation of unnatural amino acids into this biosynthetic process is rare due …
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Studies on the Vibrio alginolyticus sucrose utilization system cloned into Escherichia coli
… by plasmid pVSlOO were determined by DNAdirected cell-free protein synthesis. The capsule, produced by transformed and untransformed E. coli JA221 cells, was shown to be independent of the presence of plasmid pVSlOO. The sucrase activity assay was optimized with respect to time, pH, temperature …
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Cell-free platform for the functional characterization of intracellular ion transporters
In biotechnology, the study of membrane proteins is of high interest due to their unique physical properties used as invaluable tools for the development of new therapeutics. However, honing down on the structural and functional properties of membrane proteins is no easy feat, owing to challenges …
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Strategies to detoxify the mycotoxin deoxynivalenol and improve food safety in the U.S.
… microorganisms that detoxify DON, (2) use a cell free protein synthesis (CFPS) system to study enzymes that modify DON, (3) engineer yeast to detoxify DON with a metabolic engineering strategy, and (4) deliver a high school unit to teach high school students about mycotoxins in food. In …
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Factors Affecting Translation of Messenger RNA's In Vitro: Use of a GTP Analogue to Investigate Rates of Polypeptide Chain Elongation
… order of addition of amino acids to a growing protein is determined by the codon sequence of a messenger RNA molecule. This translation process was studied in vitro with a cell-free protein synthesis system derived from Escherichia coli. The rate of protein synthesis was proportional to the …
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Design and Discovery of Novel Bacteriocins
… 4-HB Bacteriocins in silico from the vast public protein databases which have been populated in recent years, encompassing over 580 million sequences. A total of 234 naturally occurring candidates were identified using a combination of sequence-based and structure-based homology search strategies, …
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New Methods of DNA Assembly, Gene Regulation with a Synthetic sRNA, and Cyanobacterium Phenotype Monitoring with Raman Spectroscopy
… to improve genetic engineering of microbial cells. In this matter, λ-PCR (lambda-PCR) was developed enabling the construction of plasmid DNA. This technique allows DNA assembly and manipulation (insertion, substitution and/or deletion) at any location of a vector. λ-PCR addresses the need for …
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Computational Modeling of Planktonic and Biofilm Metabolism
… the metabolism of planktonic and/or biofilm cells via multi-omics approaches (e.g., transcriptomics and proteomics analysis). However, these approaches are limited to provide the direct description of intracellular metabolism (e.g., metabolic fluxes) of microorganisms. Therefore, in this …