Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"cell-free assay"”.
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The establishment and characterization of an improved cell-free assay for exocytosis in neuroendocrine PC12 cells
… In dieser Arbeit wurde ein zellfreier Exozytose-Assay entwickelt.Auf beschichteten Objektträgern wachsende PC12-Zellen wurden durch einen Ultraschallpuls in einer kleinen Kammer direkt auf dem Objekttisch eines Fluoreszenzmikroskops aufgeschlossen. Durch Ca2+ konnten eGFP-beladenen Vesikel der so …
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Mechanisms of toxicity and carcinogenicity of three alkylanilines
… to assess in vitro DNA adduct formation with the cell-free and cell-based assays. Accelerator Mass Spectrometry (AMS) was used for detection and semi-quantification of DNA adducts formed by 14C-labeled alkylanilines. Data indicated 3,5-DMA formed high levels of DNA adducts, suggesting that it is a …
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Developing 1,2,3,4-tetrahydro-5H-aryl[1,4]diazepin-5-ones and Related Scaffolds as Poly-(ADP-ribosyl) Polymerase (PARP) Inhibitors and Exploring Their Targeted Polypharmacology with Kinases
… inhibition, including PI3K/AKT/mTor pathway, and cell-cycle checkpoints (such as CDKs, CHK, and Wee) are being tested. In this context, the synthetic lethality of cyclin-dependent kinase 1 (CDK1) and PARP1 is known. Evaluation of PARP1 and CDK1 pharmacophores led to the development of the …
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Development of Catalytic RNAs as Potential Anti-HIV-1 Agents
… in vitro and to inhibit viral proliferation intracellularly. Two classes of ribozymes that incorporate a "hammerhead" catalytic center were utilized, eotide elements in the viral sequence to provide half of the The first class used nuclcatalytic center. A complementary strand harboring the …
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Development of aminoquinoline-benzimidazole hybrids and their organometallic complexes as antimicrobial agents against Plasmodium falciparum and Mycobacterium tuberculosis
… against the Chinese hamster ovarian (CHO) cell-line. In addition, the compounds were also evaluated against Mycobacterium tuberculosis. A series of aminoquinoline-benzimidazole hybrid compounds were prepared. The 2-position of the benzimidazole was substituted with an organic phenyl or …
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Development of less toxic analogues of fascaplysin as novel therapeutic agents for the treatment of cancer
… All of these aberrations can cause deregulated cell growth, resulting in tumour formation. Therefore, a small synthetic molecule or a natural product that specifically and potently inhibits the kinase activity of Cdk4 in vitro and prevents cell growth and tumour volume in vivo could be of …
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Molecular Mechanisms of Inward and Outward Budding From Multivesicular Endosomes
… transporters, ion channels, receptors) on the cell surface can modify their response to extracellular cues and allow for cellular adaptation to environmental conditions. The fate of membrane proteins that are internalized from the plasma membrane and arrive at the limiting membrane of the late …