Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"cell-concentration"”.
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Argentaffin Cell Concentration and Villus Area of the Intestine of the Irradiated Guinea Pig
… It halts the mitoses in the crypts except for cells dividing at the time of irradiation. Often the daughter cells of the latter are abnormal or the division is aborted. | Further radiation damage in the intestine includes shrinkage of the villi, shallowing of the crypts, and vacuolization and …
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Modeling and simulation of non-linear bioelectronic systems applied to cell culture assays
… depending on the type of biological sample (cells, tissues, blood, etc.). In this thesis, we improve the accuracy of the ECIS (electrical cell-substrate impedance spectroscopy) technique of bioimpedance (BI) measurement of a monolayer cell-culture (CC), which measures the electrical response …
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Measuring the absolute concentration of particles in suspension using high frequency B-mode ultrasound imaging
Concentration measurement of particles in suspension is an important procedure performed in biological and clinical laboratories. Existing methods based on instruments such as hemocytometers, coulter counters, and flow cytometers are often laborious, destructive, and incapable of in vivo …
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Metabolic flux analysis and population heterogeneity in mammalian cell culture
… were used to develop relations between mammalian cell physiology and specific culture environments and to formulate strategies for increasing cell culture performance. Mitochondrial characteristics associated with respiration, membrane potential, and apoptosis along with physiological state …
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Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems
… production by HPCHO Chinese hamster ovary cells and IB4 hybridoma cells in batch, fed-batch, and three perfusion systems: the stirred ceramic membrane reactor (SCMR), the alternating tangential flow (ATF) hollow fiber membrane system, and the external spin filter (ESF) system. The batch …
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The aggregation of clay minerals and marine microalgal cells : physicochemical theory and implications for controlling harmful algal blooms
… is based on the mutual aggregation of algal cells and mineral particles, leading to the formation of large flocs that rapidly settle to the ocean floor. This work investigated the effectiveness of various domestic clays against a number of bloom-forming species from the United States. …
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Selective Isolation of Circulating Tumor Cells in Antibody-Functionalized Microsystems
Attachment of circulating tumor cells in microfluidic devices functionalized with proper antibodies was studied. Under static experimental conditions, microchambers were utilized to study the parameters such as cell suspension concentration, incubation time or ambient temperature that may affect …
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Identification of Human Adrenal Carcinoma Cells Using Photoacoustic Flow Cytometry
… absorbance wavelength of human adrenal carcinoma cells, as well as the minimum cell concentration needed in samples using photoacoustic flow cytometry (PAFC). PAFC generates ultrasonic waves when particles under flow absorb laser light. Due to the yellow color in adrenal cancer cells, we …
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Assessing CyAN satellite accuracy: a closer look at Kansas' aquatic boundaries
… CyAN’s performance in estimating cyanobacteria concentrations at the water’s edge as well as when classified these into KDHE’s HAB levels: Watch, Warning, and Hazard. Findings from the cell concentration analysis demonstrate that the satellite data's accuracy varies significantly with lake …
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Phytoplankton growth and diel variations in beam attenuation through individual cell analysis
… from flow cytometric analysis of individual cells, was determined and found to show similar diel patterns for populations of pico- and nanophytoplankton measured in the equatorial Pacific and the Sargasso Sea. The cell concentration patterns do not generally correspond to those of beam …
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ESTABLISHING PTERIDINE METABOLISM IN A BREAST CANCER CELL MODEL AND QUANTIFICATION OF SILVER NANOPARTICLE INTERACTIONS WITH YEAST CELLS USING MASS SPECTROMETRY
… quantitative insights that can be gleaned from cellular analyses, with applications in cancer and nanoparticle research. In the first part of the research presented, a recently developed HPLC-MS/MS method was advanced to elucidate pteridine metabolism in an isogenic progressive MCF10A breast …
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Physics and Applications of a PDMS Based Centrifugal Microfluidic System
… from whole blood. The resulting residual cell concentration was less than 0.5%. In the last part, a micromixer was developed based on the similar principle. The results show that the flyball governor system can effectively agitate the chaotic mixing of the sample liquids by periodically …
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Changes in microvascular hematocrit during post-occlusive reactive hyperemia: descriptions and mechanisms
… T[Hb+Mb] must reflect alterations in red blood cell concentration in the microvasculature, i.e., microvascular hematocrit. In Chapter 4 comparisons were made between brachial artery blood flow, cutaneous and skeletal muscle flux and T[Hb+Mb]. The conduit artery response was faster than the …
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Production, purification and properties of Bacillus thuringiensis neutral protease
… the spore and toxic paraspore (crystal) an extracellular proteolytic enzyme. This study concerns the conditions for production, the.purification and the properties of this enzyme. It was found in studies relating to production of protease that B. thuringiensis var. kurstaki (HD-1) produced a …
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Cell Growth Paramenters and Sister Chromatid Exchange Level in Fibroblasts from a Patient Diagnosed with Bloom Syndrome
… responses of the KB, normal, and Bloom Syndrome cell lines to fibroblast qrowth factor were determined by culturing the fibroblasts over a seven-day period in the presence of three concentrations of fibroblast growth factor in medium supplemented with two levels of fetal bovine serum. Cell …
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Phycoremediation of artificial bathroom greywater in village houses using microalgae botryococcus sp.
… phycoremediation. To optimize Botryococcus sp. cell concentration, pH and the efficiency of laboratory scale treatment system with Botryococcus sp. was observed. The first objective results shown that NO3-N and PO4-P were 1.03-7.54 & 0.12-22.7 mg/L respectively and 63 L/c/day was discharged to …
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Injectable hyaluronic acid scaffolds for cartilage tissue engineering
… Because of the lack of blood supply and low cell concentration within the tissue, cartilage has very limited regenerative ability. Although current treatments can provide symptomatic relief, the results vary greatly among individuals, and newly formed tissue often does not duplicate the …
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Picophytoplankton of the Northeast U.S. Shelf: community composition and dynamics
… both to the physiology of the individual cells and to the structure of the Northeast U.S. Shelf (NES), a productive and economically important coastal ecosystem. To identify the drivers of seasonal changes in picophytoplankton abundance, I first estimate daily division and loss rates for a …
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Sparse Sample Detection Using Magnetic Bead Manipulation on a Digital Microfluidic Device
… and antigen enables the binding between target cells and antibody-‐‑ coated magnetic beads, hence achieving sample capture. For the second step, fluorescent detection is achieved via fluorescent signal measurement and magnetic bead manipulation. In those two steps, a total of three functions …
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Investigation of Bacillus subtilis as a Biopesticide Against Botrytis cinerea
… ssp. KLB. The <em>B. subtilis</em> KLB concentration required to completely inhibit <em>B. cinerea</em> was 9.1x10<sup>4</sup> CFU/mL when <em>B. subtilis</em> KLB was inoculated 48 hours before <em>B. cinerea</em>, 1.3x10<sup>5</sup> CFU/mL at 24 hours, and 3.2x10<sup>6</sup> CFU/mL …
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