Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 121 for “"cell volume"”.
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Controlled loading of cryoprotectants (CPAS) with cell volume constraints
… depends on knowing the permeability of the cell membrane to water and cryoprotective agents. Due to the large size of oocytes that requires microscopy methods which could be difficult to implement, micro fluidic device is presented in this study based on Coulter-principle to simultaneously …
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Negative fluorescence : an optical flow cytometric technique for cell volume determination
Thesis (M.S.)--Massachusetts Institute of Technology, Dept. of Electrical Engineering and Computer Science, 1981.
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Simulated impact trauma and osteoarthritis: the role of cell volume and mechanotransduction
… 1.14 N) induced by a drop tower device caused cell death from as early as 2 min. Pre-incubation in hypertonic media protected chondrocytes from cell death, whereby at 30 min, death was decreased from 9.22 % to 3.42 % (p<0.01), thus implicating volume regulatory changes as a potential key …
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Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton
The relationship between initial intracellular pH (pHi) and associated cell volume change was investigated by simultaneous measurement of pHi and cell volume with fluorescence imaging in polarized fungiform taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, …
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Development of dielectric spectroscopic monitoring methods for the prediction of viable cell density and volume in mammalian cell culture
… methods for the real-time prediction of viable cell density and viable cell volume in Chinese hamster ovary cell cultures. This work focuses on the use of Biomass Monitor for the monitoring of biomass. Two dual frequency settings and 2 temperatures were examined. The effects of interferences …
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Convective intracellular macromolecule delivery for cell engineering applications
Efficient intracellular delivery of target macromolecules remains a major obstacle in cell engineering, cell labeling, and other biomedical applications. Current standard methods of intracellular delivery, such as viral transduction and electroporation, do not meet the growing needs in the cell …
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Genetic and molecular studies of cell-autonomous execution during programmed cell death in C. elegans
Apoptosis or programmed cell death was originally defined by evolutionarily conserved morphological characteristics that include shrinkage of cell volume and chromatin condensation. Apoptosis functions as a highly controlled mechanism for the elimination of unwanted or damaged cells and is …
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Crystal chemistry of the humite minerals
… and Ca for Mg has the same effect on the unit cell volume (normalized to half the mean anion-anion distance along the normal to (001)) in minerals with the humite structure as it does in minerals with the olivine structure. Fifty-five humite mineral samples have been analyzed by microprobe …
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Discovery of Novel Mechanisms Regulating Cancer Extravasation in the Chorioallantoic Membrane Model
… process that begins with the invasion of tumour cells into the stroma and migration towards the blood vessels. Tumour cells that have entered the bloodstream must then survive and leave by a process known as extravasation. Finally, extravasated cells proliferate and establish the secondary site …
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Temporal profile of gonadal steroids in populations of roosters divergently selected for mating frequency
… as opposed to the low mating line. Packed cell volume and body weight measurements were obtained at the termination of the experiment. Significant differences were found between the mating lines for both characteristics; the high mating line exhibited a greater mean packed cell volume and a …
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The effects of heating and dehydration on the crystal structure of hemimorphite up to 600{u00B0}C
… throughout the experiment was Imm2, Z=2, and the cell dimensions at each temperature, in the above-mentioned order, are: a=8.337(5), b= 10.724(6), c=5.116(4)Ȧ; a=8.268(5), b=10.784(8), c=5.113(3)Ȧ; and a=8.206(4), b=10.815(6), c=5.089(2)Ȧ. Anisotropic least-squares refinements resulted in …
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Volumetric reconstruction of tissue structure from two-dimensional microscopy images
Cell morphology of tissue is naturally three-dimensional. Most current methods for tissue analysis use two dimensional histological images of the tissue samples, restricting the analysis to 2D. Existing approaches do not provide essential three-dimensional information such as cell volume, shape and …
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The role of DEL1 in chondrocyte expansion and mechanotransduction: an investigative study
… chondrocytes were cultured at low density (1x104cell/cm2) and subcultured on days 9 (P1), 14 (P2) and 21 (P3). A loss of a differentiated phenotype was observed in P1 with a reduction in sphericity from 0.72±0.02 to 0.52±0.03. Changes in cellular dimensions in response to 2D culture were …
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Aging Changes Cell Mechanics and Dynamics with a Backbone of Cytoplasmic Crowding
… effect of aging could be better understood in cells, which are the fundamental building blocks of life. In particular, because the cytoplasm comprises approximately 70% of cells and its physical properties strongly influence biological processes, understanding its mechanics provides a more …
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Element substituted aluminophosphates
… of CoAPO-5 is obtained by the change in its unit cell volume relative to AlPO₄-5, and by diffuse reflectance spectroscopy. CoAPO-5 also contains an appreciable quantity of extra-framework cobalt occluded in the pores, and/or as balancing cations. Evidence for this was obtained from ion exchange, …
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Chloride Channels in Normal Human Blood Monocytes and a Macrophage-like Cell Line
Phagocytic cells serve critical roles in the body’s immune response by recognizing, phagocytosing and destroying foreign particles. A major class of phagocytic cells is composed of monocytes and macrophages. During inflammation there is release of several inflammatory mediators and chemokines, …
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Dynamic Properties of Chondrocytes
… and absorbs mechanical impact. Chondrocytes, the cells within cartilage, experience continuous mechanical loading in vivo and adapt their volume and shape accordingly, a process that is essential for maintaining cartilage health and function. Volume and shape changes in chondrocytes are key to …
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Mechanisms of ion channel activation in primary cultured and clonal cell lines
… C1 permeability of ROS 17/2.8 osteoblast-like cells was investigated using the patch-clamp technique. Hypotonic shock produced cell swelling that was accompanied by large amplitude, outwardly rectifying, currents that were active across the entire physiological range of membrane potentials (-80 …
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Controlled cross circulation: effects on donor hemodynamics
… max, pulmonary capillary wedge pressure, blood volume, systemic vascular resistance, heart rate, total protein, platelet count, and white blood cell count did not change significantly. Donor cardiac output, end diastolic volume, central venous pressure, stroke volume, mean arterial blood …
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Mechanical loading and cartilage physiology
… osteoarthritis (OA). This study investigated the cellular responses of chondrocytes to mechanical impact, and the effects of possible chondroprotective agents for OA preventative strategies in individuals exposed to high impact, repetitive loading. Single-impact mechanical trauma (force 1.14 N, …
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