Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"cell uptake"”.
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The effect of manipulating apoptotic cell uptake on their immunogenicity
… system to differentiate between apoptotic tumour cells and cells rendered apoptotic as part of homeostasis prevents a successful response being mounted against tumours. Apoptotic cells are cleared rapidly by professional phagocytes thus preventing the release of potentially inflammatory or …
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Enhancing the radio sensitivity of prostate cancer using RALA Gold nano-complexes
… systemic circulation, poor stability and target cell uptake. This thesis employs the cell penetrating peptide RALA to enhance AuNP cell internalisation and in turn radiosensitivity of PCa. RALA-AuNP have undergone extensive characterisation following synthesis, proving optimal for in vitro and in …
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Guanidinium Compounds: Synthesis, Oxoanion Binding, and Cellular Delivery
The delivery of biological molecules into cells has been an issue of importance in both chemical biology and drug discovery. One method used to transport biologics into cells is the cellpenetrating peptide (CPP). This arginine-rich peptide forms strong interactions with the cell surface through …
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Incorporation and analysis of bilayer constituents in nanodiscs
… Nanodisc bilayer was analyzed and preliminary cell uptake studies were performed. Finally, the incorporation of proteins from rat liver and fetal bovine brain membranes into Nanodiscs to create soluble membrane protein libraries is discussed.
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Engineering periodic short hairpin RNA delivery systems for enhanced therapeutic efficacy
… protect siRNA from degradation, promote target cell uptake, and facilitate endosomal escape into the cytoplasm, where RNAi occurs. However, in vivo instability, low silencing efficiency, undesired toxicity, and immunogenicity remain challenges for current siRNA delivery systems, particularly as …
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SYNTHESIS, SURFACE MODIFICATION, AND CHARACTERIZATION OF BIOCOMPATIBLE MULTIFUNCTIONAL NANOPARTICLES
… first produced in 1990s become an alternative excellent candidate to load biomolecules, organic dyes, and magnetic nanocores due to their special porous structure. The challenges of developing such multifunctional nanoparticles include: poor cell uptake due to aggregation of NPs, low fluorescent …
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GLYCOSAMINOGLYCAN COATED GOLD NANOPARTICLES FOR TARGETED BREAST CANCER THERAPY
… that is overexpressed in most types of cancer cells, including breast cancer. Glycosaminoglycans (GAGs), such as hyaluronic acid (HA) and chondroitin sulfate A (CSA), are known as binding ligands of CD44 receptor and were used as non-toxic targeting ligands in targeted cancer therapy. Gold …
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Cell penetrating peptide for ocular drug delivery
… and the vasculature of the eye, leading to cell death, vascular permeability and vision loss. Cx43 HC block has proven to be effective in inhibiting the inflammatory cascade in various in vitro and in vivo ocular models. Gap19 is an intracellularly acting connexin mimetic peptide, said to be …
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Evaluating the use of cross-linked PVA nanoparticles for gene and drug delivery
… theoretically, permit endocytosis by eukaryotic cells. However, during in vitro transfection studies no reporter (GFP) gene expression was noted. The collective evidence from electroporation, fluorescent-DNA-tagging, Lipofectin® or calcium phosphate chimeric and chloroquine experiments suggest …
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Assembly, characterization and evaluation of a 3rd generation nanoparticle based drug carrier for metastatic breast cancer treatment
… specifically target and enter the tumor cells and slowly release the drug thereafter. Advanced nanoparticle drug carriers are studied driven by the need of a more efficient drug delivery. The 3rd generation of nanoparticle based drug carriers are recently developed. They usually consist …
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Development of Shell-Crosslinked Knedel-like Nanoparticles as Intracellular Delivery Vehicles and Gene Regulation Agents
… guest molecules or guest structures into the cell. Detailed synthetic procedures for and characterization of well-defined block copolymers, as well as the nanostructures resulted from their self-assembly are reported. The nanoparticles exhibited different but controlled sizes and shapes, …
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Nanoclusters for Cancer Therapy: Assesment of Their Reactive Oxygen Species Generation and Stability
… oxygen species, resulting in targeted cancer cell death. To be an effective photosensitizer, clusters must have long-lived excited states that can interact with oxygen to produce reactive oxygen species. This interaction between the excited state of nanoclusters and oxygen remains relatively …
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EXPANDING APPLICATIONS OF IRON OXIDE NANOPARTICLES BY SURFACE FUCNTIONALIZATION: FROM MAGNETIC RESONANCE IMAGING TO NANO-CATALYSIS
… an order of magnitude around pH 5. The efficient cell-uptake (~3 million/cell) and low cytotoxicity of G1 to G3-SPIONs were demonstrated on HeLa cell cultures. The strong R2* effects were observed indicating the SPION clustering in HeLa cells. Two SPION-oligonuleotide conjugates were synthesized …
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A New Approach to Drug Delivery: Non- Peptidic, High Load Macrocyclic Alternatives to Cell Penetrating Peptides
… functionalising calixarenes. The phenomenon of cell penetration is of interest where a molecule has an intracellular target, for example gene therapy, delivery of cytotoxic agents or cellular imaging. Chapter 2 introduces the mechanisms of cell uptake and the design and applications of cell …
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Approaches to the assembly of potent therapeutic agents for the treatment of myotonic dystrophy
… the MBNL1-rCUGexp both in vitro and in DM1 model cells, and is able to improve two distinct disease phenotypes in a DM1 Drosophila model. However, its relatively low maximum tolerated dose in mice and limited cell uptake provide insights into directions for future development. In addition, a …
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An ecological study of waste stabilization ponds in Werribee, Australia, with special reference to nutrient and plankton dynamics
… not only stabilize the nutrients through direct uptake, they also produce oxygen via photosynthesis, facilitating aerobic degradation and nitrification by aerobic and nitrifying bacteria respectively. In the pond series at Werribee, ammonia in the raw sewage was completely removed through …
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Dendrimer Based Nanotherapeutics For Ocular Drug Delivery
… in suppressing VEGF production in hypoxic RPE cells. Dendrimers targeted activated microglia/macrophages and RPE and retained for a period of 21 days in I/R mice model. The relative retention of intravitreal and intravenous dendrimers was comparable, if a 30-fold intravenous dose is used; …
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Trajectory features as surrogate measures of the nanoparticle-microenvironment interaction space
… some successes, including FDA approval of micelle and liposomal formulations for the delivery of strongly hydrophobic anticancer drugs, the translation of these technologies has slowed; in 1995, the ratio of animal studies to FDA-approved drugs was 19:1 and the ratio of animal studies to …
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Solid dispersions: formulation, characterisation, permeability and genomic evaluation
… as compared to drug alone across Caco-2 cell monolayers. From the cell uptake studies it was shown that PEG 8000 enhanced rhodamine123 uptake which suggested that PEG 8000 may increase the permeability of these drugs in solid dispersions. Gene expression profiles analyzing the expression …
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Improved iron and zinc availability in sorghum by phytate reduction through genetic modification, fermentation and phytase addition
… a dialysability assay (bioaccessibility), Caco-2 cell (uptake) study and an in vivo suckling rat pup model (absorption). A mineral solubility assay was used to analyse the soluble minerals in the raw cereal wort. GM low phytate (approx. 30-40% reduction) non-tannin and tannin sorghums and their …
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