Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 14 of 14 for “"cell swelling"”.
-
Intracellular pH, the Proximate Signal for Cell Volume Changes that are Mediated by the Actin Cytoskeleton
The relationship between initial intracellular pH (pHi) and associated cell volume change was investigated by simultaneous measurement of pHi and cell volume with fluorescence imaging in polarized fungiform taste receptor cells (TRCs) loaded with BCECF in vitro. Ammonium pulses caused a brief, …
-
Glutamate Receptor-Mediated Taurine Release From The Hippocampus During Oxidative Stress
… and has been directly linked to hippocampal swelling and cytotoxic brain edema in vitro. Swollen brain cells activate volume regulatory mechanisms including a significant efflux of the endogenous sulfonic amino acid taurine via volume-regulated anion channels (VRACs). Studies in brain slice …
-
Evaluation of Zinc Toxicity Using Neuronal Networks on Microelectrode Arrays: Response Quantification and Entry Pathway Analysis
… generally preceded morphological changes. 20% cell swelling was correlated with 50% activity loss. Cultures pretreated with the GABAA receptor antagonists bicuculline (40 mM) and picrotoxin (1 mM) lacked the initial excitation phase. This suggests that zinc-induced excitation may be mediated by …
-
Testing the Gliocentric Hypothesis of Brain Pathology
… cause of death and disability worldwide, causing cell death and tissue damage. All cell types are affected by ischaemia, however until recently, the impact of acute ischaemia has been studied from a neuronal point of view. Currently, there is a greater interest in glial cells such as astrocytes …
-
The mechanisms of aquaporin expression and translocation in cerebral pathologies
… membranes and are essential for maintaining cellular integrity. In humans, the AQP family consists of 13 established members (AQP0-12). AQPs 1, 3, 4, 5, 8, and 9 are collectively known as "cerebral AQPs" as they are known to be present in the mammalian brain. AQPs are distributed throughout a …
-
Measurement and Analysis of Calcium-Dependent Exocytosis in Giant Excised Membrane Patches
… exocytosis was studied in both excised and whole-cell patch clamp with emphasis on the rat secretory cell line, RBL. Capacitance and amperometric recordings show that secretory granules (SGs) containing serotonin are mostly lost from excised patches. Small vesicles that are retained (non-SGs) do …
-
Mechanisms of ion channel activation in primary cultured and clonal cell lines
… C1 permeability of ROS 17/2.8 osteoblast-like cells was investigated using the patch-clamp technique. Hypotonic shock produced cell swelling that was accompanied by large amplitude, outwardly rectifying, currents that were active across the entire physiological range of membrane potentials (-80 …
-
Molecular mechanisms of gating and selectivity in transport channels
… while preventing unnecessary loss of other cellular material. The nuclear pore complex (NPC), and the mechanosensitive channel of small conductance, MscS are the focus of the research presented in this thesis. NPCs are sole gateways for passage of material across the nuclear envelope of …
-
Protein Turnover in Rat Skeletal Muscle During In Vitro Hypo-Osmotic Stress
Hypo-osmolality influences tissue metabolism, but research on protein turnover in skeletal muscle is limited. The purpose of this investigation was to examine the effects of hypo-osmotic stress on protein turnover in rat skeletal muscle. We hypothesized increased protein synthesis and reduced …
-
Understanding Primate Immunity to African Trypanosomes Through the Characterization of Trypanosome Lytic Factor Formation and Function
… in Sub-Saharan Africa. These parasites are unicellular, extracellular parasites, that infect humans, cattle, and other livestock. Humans and some non-human primates are protected against most species of trypanosomes due to an innate immunity factor, Trypanosome Lytic Factor (TLF). This modified …
-
The Role of Polyamines in Osmotic Stress Tolerance in Gulf Killifish Fundulus Grandis
… are highly polycationic at physiological intracellular pH. Polyamine levels are regulated by the coordinated actions of arginase (Arg), ornithine decarboxylase (Odc), and polyamine oxidase (Pao). Although little is known of their functions in fish, polyamines have been implicated in diverse …
-
Differentielle Expression und Funktion eines glialen Kaliumkanals (K<sub>ir</sub>4.1, KCNJ10) im Rückenmark und Bedeutung in einem neurodegenerativen Krankheitsmodell
Innerhalb des letzten Jahrzehnts wurde immer deutlicher, dass einwärts-gleichrichtende Kaliumkanäle (Kir) neben ihrer klassischen Rolle im Aufbau und der Konstanthaltung des Ruhemembranpotentials weitere zellspezifische Aufgaben erfüllen.In der vorliegenden Arbeit konnte mittels transgener …
-
Aqueous extracts of Dodonaea viscosa induce potent and selective cytotoxicity in DLBCL cells
… among both males and females. Diffuse large B cell lymphoma (DLBCL), an aggressive B-cell derived cancer is the most prevalent cancer within the NHL group of cancers. DLBCL can be grouped into two main subtypes, namely the germinal-centre B cell (GCB) and the activated-B cell (ABC) subtypes, …
-
MORPHOLOGICAL FEATURES OF THE REPRODUCTIVE ORGANS IN DOMESTIC SPECIES IN RELATION TO WATER MOVEMENT CONTROL, HYPONUTRITION AND ENVIRONMENTAL INFLUENCES IN FETAL AND PREPUBERTAL LIFE, AND METABOLISM REGULATOR FACTORS.
… membrane proteins that are able to make the cell membrane 10 to 100 fold more permeable than the membranes lacking such channels. Based on studies with AQP1, it appears that AQPs exist in the plasma membrane as homotetramers. Each AQP monomer contains 2 hemi-pores, which fold together to form …