Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 30 for “"cell surface protein"”.
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A Study of Cell Surface Change After Viral Transformation by Iodination and Cell-Cycle Dependent Phosphorylation of Sv-40 Large Tumor Antigen
… two parts. Part I deals with iodination of the cell surface protein before and after viral transformation and Part II deals with cell-cycle dependent phosphorylation of the large tumor antigen induced by SV-40 in human cells.
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Characterization of a single-chain anti-T cell receptor antibody and a single-chain T cell receptor
The T cell receptor (TCR) is a cell surface protein that specifically binds to a foreign antigen on the surface of another cell. The TCR is made up of two disulfide-linked chains (called $\alpha$ and $\beta$) each containing a variable and a constant domain. The tertiary structure and binding …
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CHIC2 and STUB1 regulate interferon-γ receptor cell surface expression
The regulated expression of cell surface receptors is essential for cellular homeostasis and defective in disease states. Receptor regulation is particularly important as receptor internalization limits the duration and intensity of substrate signaling. Interferon-γ (IFN-γ), a critical antiviral …
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Investigating The Role of Cd109 In Pancreatic Ductal Adenocarcinoma
… on a cohort of four patient derived PDAC cell populations and our data shows a cell surface receptor CD109 to be a common vulnerability, the biologic role of which in PDAC is yet unstudied and largely unknown. We hypothesized that CD109 expression provides PDAC cells with a survival …
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Evaluation of new succinimide-based small molecules as alphavbeta3 integrin antagonists: from design to preliminary biological testing
Integrins are a family of cell surface protein receptors consisting of two subunits, called α and β, involved in several biological processes, including regulation of signalling between cells and other cells or ECM. Integrins are overexpressed in diseases, first of all cancer. Being able to bind …
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Investigating the molecular mechanisms of the interactions between Lactobacillus reuteri strains and intestinal mucus
… investigated the occurrence at the genetic and protein level of two specific cell surface proteins, MUB and Lar_0958, expressed by the ATCC 53608 strain and human strains, respectively and their role in mediating mucus binding and autoaggregation ability of L. reuteri strains, as determined by …
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Role of c-Cbl in Invasion of Mammalian Cells by Rickettsia Conorii
[SPECIFIC AIMS] Rickettsia conorii is an intracellular bacterium that causes Mediterranean spotted fever. R. conorii is transmitted from ticks to humans and invades vascular endothelial cells. A recent publication (Martinez et al., 2005) has identified Ku70 as a host cell receptor that binds to …
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Microarray Analysis Identifies Novel Cholesterol Regulated Genes, Including Pcsk9 Which Regulates LDL Receptor Function and LDL Cholesterol Levels
… levels is necessary for survival of individual cells and the normal functioning of multicellular organisms. Many of the genes important in these processes are regulated at a transcriptional level by the sterol regulatory element binding proteins (SREBPs) and liver X receptors (LXRs). This thesis …
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Cell surface analysis of the basidiomycete yeast cryptococcus neoformans
Cell surface properties of the basidiomycete yeast Cryptococcus neoformans were investigated with a combination of novel and well proven approaches. Non-specific cell adhesion forces, as well as exposed carbohydrate and protein moieties potentially associated with specific cellular interaction, …
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Structural Investigations of Porcupine and Dispatched-1 in Wnt and Hedgehog Morphogen Biogenesis
… development, and its ligand is secreted by a cell surface protein named Dispatched-1 (DISP1). I report the cryo-EM structure of the DISP1 in complex with dual lipid-modified HH ligand, suggesting how DISP1 engages with HH ligand. DISP1 contains 12 transmembrane helices and two extracellular …
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Rational design and directed evolution of probe ligases for site-specific protein labeling and live-cell imaging
… superior photophysical properties to fluorescent proteins and are much smaller. However, in order to use these probes for live-cell protein imaging, highly specific labeling methods are required. Here, we will describe three efforts to re-engineer the E. coli enzyme, lipoic acid ligase (LplA), to …
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Dissecting the Molecular Basis of the Antiphospholipid Syndrome
… if aPL-induced alterations in endothelial cell phenotype are mediated by aPL actions on eNOS. Normal human IgG (NHIgG) and human IgG containing polyclonal aPL were obtained from healthy individuals and APS patients, respectively, and purified by protein G-sepharose chromatography. We found …
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Regulation of Clathrin Mediated Endocytosis and Its Role in Cancer Progression
… is a multistep process requiring cancer cell signaling, invasion, migration, survival, and proliferation. These processes require dynamic modulation of cell surface proteins by endocytosis. Given this functional connection, it has been suggested that endocytosis is dysregulated in cancer. …
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Investigating senescence and senescence-escape in cancer cells
… role, the presence of senescent tumour cells and the ability of cancer cells to escape from senescence could potentially lead to therapy resistance and tumour recurrence. While a wide range of chemotherapeutic drugs are known to induce senescence in cancer, the effect of TIS in tumour …
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Mechanisms of Virulence Regulation in Porphyromonas gingivalis W83
<p>The expression of extracellular proteolytic activities is highly regulated in both prokaryotic and eukaryotic systems. This regulation can occur at multiple levels including expression of the protease genes, secretion, processing of an inactive secreted precursor to its active form and/or the …
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Toxicity, morphological changes, and dissipation of oil sands naphthenic acids in Chlamydomonas reinhardtii
… ascertain the toxic effects of NAFCs on the unicellular green algae Chlamydomonas reinhardtii wild type (WT) and two cell wall mutants: CC-400, which retains the innermost and outermost glycoprotein wall layers, and CC-3395, believed to be completely naked. The presence of the cell wall was …
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Development of a MACS-based strategy for isolating rare cell populations from animal tissue for transcription factor studies
Two major challenges in studying specific cell populations of embryos or adult organs are: a) getting the sample sufficiently pure for the cells of interest; b) getting sufficient quantities of these cells. Current methods used for isolating specific cell populations from tissues are either too …
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Ctla-4 transcriptional activation: regulation of induced expression
… T-lymphocyte antigen 4 (CTLA-4) is a T cell surface protein that is homologous to CD28 and binds to the B7 family of ligands. Unlike CD28, CTLA-4 interaction transmits a negative signal in T cells, leading to suppression of proliferation. CTLA-4 is constitutively expressed on regulatory …
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Investigation of the interactions between Trypanosoma congolense invariant surface glycoproteins and components of the mammalian immune system
African trypanosomes are single-celled eukaryotic pathogens of humans and animals. As they are extracellular in vertebrate hosts, their cell surface forms the primary host-pathogen interface. Invariant surface glycoproteins (ISGs) are a large family of cell surface proteins with recent expansion …
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Identification and Characterization of Rare Variants in Cholinergic Nicotinic Receptor Genes and their Contribution to Substance Dependence
… We find several variants that alter cellular response either to nicotine or acetylcholine as well as many variants that alter cell-surface protein expression as measured by cell-surface ELISA without altering either <italic>CHRNB4</italic> mRNA or total β4 protein as measured by …
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