Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 68 for “"cell surface expression"”.
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CHIC2 and STUB1 regulate interferon-γ receptor cell surface expression
The regulated expression of cell surface receptors is essential for cellular homeostasis and defective in disease states. Receptor regulation is particularly important as receptor internalization limits the duration and intensity of substrate signaling. Interferon-γ (IFN-γ), a critical antiviral …
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N-linked glycosylation of ether á go-go potassium channels: effects on cell surface expression and functional properties
… hervorgerufen, da der gleiche Effekt bei der Expression des Wildtyp-Proteins in Zellen mit mangelnder Glykosylierung beobachtet wird. Obwohl das unglykosylierte Protein teilweise im Endoplasmatischen Retikulum festgehalten wird, muss ein wesentlicher Bestand der Mutanten-Kanäle an der …
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The role of C-terminal cysteines in regulating human proteinase-activated receptor-1 function
… they carry their own tethered ligand within extracellular N-terminus. To activate the receptors, the tethered ligand is exposed by proteolytic cleavage which subsequently binds to the extracellular loop 2 to trigger receptor function. Four members have been identified so far in this group—PAR1, …
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Immune reconstitution post bone marrow transplantation
… document the immune reconstitution following T-cell depleted bone marrow and peripheral blood stem cell transplantation and to compare this with the recovery following autologous grafts. to document the cell surface expression of CD95 in an attempt to comment on the role played by FAS mediated …
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The role of intracellular and extracellular cysteines in regulating human proteinase-activated receptor-2 (hPAR2) function
… have found that activation of hPAR2 by mast cell tryptase, (the major PAR2 activator outside the gastrointestinal tract) can be regulated by receptor N-terminal glycosylation. In order to elucidate other post-translational modifications of hPAR2 that can regulate function, we have explored …
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Analyzing the Interactions Between Xenopus tropicalis MC2R, MC5R, and the MRAPs: Modeling the Regulation of Frog Interrenal Cells
… MRAP2. The MRAPs have an opposite effect on the cell surface expression of MC2R and MC5R. In mammals, MRAP aids MC2R but inhibits MC5R cell surface expression. This thesis aims to explore the functional relationship between MC2R, MC5R, MRAP, and MRAP2 in<em> Xenopus tropicalis</em> to determine …
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Cell-surface Tumoricidal Molecules and NF-kB in the Tumor-burdened Host
… (TNF)-a is both secreted by and expressed on the cell-surface (mTNF-a) of macrophages. When stimulated with LPS, tumor-burdened host (TBH) macrophages secrete more TNF-a than normal host (NH) macrophages. In this study, I showed that mTNF-a is elevated both in freshly isolated and stimulated TBH …
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Investigating transmembrane TNF and transmembrane p55TNFR mediated signaling in host immune function during Mycobacterium tuberculosis infection
… macrophage activation reflected by enhanced cell surface expression of MHC-II, CD80 and CD86 as well as enlargement of granulomas. Furthermore, our results show that solTNF has a regulatory function that modulates the magnitude of Th1 immune responses during acute and chronic stages of the …
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Régulation du récepteur aux opioïdes de type delta par les protéines Homer1
… However, DOP mainly localizes to intracellular compartments whereas its analgesic effects occur when translocated to the plasma membrane. The mechanisms involved in its trafficking remain also poorly known. Recently, liquid chromatographytandem mass spectrometry (LC-MS/MS) analyses …
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Molecular Dissection of Interferon-Gamma Treated Pancreatic Beta-Cells
… direct pathogenic role on the pancreatic $\beta$-cell. Early expression of IFN-$\alpha$ by the $\beta$-cell may contribute to insulitis whereas IFN-$\gamma,$ a product of islet infiltrating leukocytes, may mediate characteristic decreases in glucose sensitivity and increased cell surface …
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Genetics and functions of the SARS coronavirus spike protein
… events during virus entry and virus-induced cell-to-cell fusion. Investigations, described herein, have focused on the genetic manipulation of the SARS-CoV S glycoprotein in order to delineate functional domains within the protein. This was accomplished by incorporating single point …
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Actions of alpha-chimaerins in mechanisms relevant to dendritic spine formation and neurodegeneration
… roles for the GTPases in endocytosis and cell polarity establishment have also been defined. The neuronal proteins a1- and a2-chimaerins belong to a group of Rac and Cdc42 GAPs that inactivate these GTPases; in addition to a GAP domain, the a-chimaerins share a phosphokinase C (PKC)-like …
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TUMOR-PROMOTING FUNCTION AND MOLECULAR REGULATION OF TSPAN8 IN LIVER CANCER
… in the healthy liver. Remarkably, its expression is robustly switched on in transformed hepatocytes in two liver cancer models induced by DEN and overexpression of Neu respectively. Moreover, germline whole-body knockout of TSPAN8 significantly suppresses the tumor development in the …
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Altered Expression of Costimulatory Molecules in Behcet's Disease According to Clinical Activity
… failure to control autoreactive immune cells in the periphery may play a crucial role in BD. To produce a balanced immune response, key inhibitory costimulartory molecules that critically affect peripheral T cell tolerance and T cell function includes the cytotoxic T-lymphocyte antigen-4 …
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Novel hypothalamic G protein-coupled receptors involved in the control of reproduction
… into a mammalian N-terminal epitope-tagging expression vector. A receptor ELISA assay and receptor signalling assay were utilized to compare wild-type (WT) and mutant receptor cell surface expression and functionality in vitro. 2/6 bioinformatic tools predicted the L282V mutation would be …
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G protein-coupled receptor regulation: The role of protein interactions and receptor trafficking
… GPCR-mediated signaling operates in every human cell, and about 50% of existing clinically useful drugs act through GPCR. Kinins are proinflammatory peptides that are rapidly produced extracellularly following pathological insults and tissue damage. These peptides act through two GPCR subtypes, …
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Polyunsaturated fatty acids and immune cell functions
… with baseline. It also significantly decreased cell surface expression of CD36+, CD54+ and CD62L+ on monocytes. In contrast, there was no association between LA-rich corn oil and these adhesion molecules and chemokines suggesting that GLA and/or its metabolites are affecting the chemokines and …
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