Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 49 for “"cell spreading"”.
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Endosomal membrane dynamics underlying cell spreading: A role for the small GTPase Arf6
Cell migration is an orchestrated and highly coordinated multi-step process that is central to the development and maintenance of multicellular organisms. Dysregulated migration however, is associated with pathological states such as tumor formation and metastasis; thus a clear understanding of the …
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Identification of a Domain on the Alpha 5 Integrin Subunit Implicated in Cell Spreading and Signaling
The alpha5beta1 integrin is a cell surface receptor for fibronectin. The primary sites at which the alpha5beta1 integrin interacts with fibronectin (Fn) are the RGD (Arg-Gly-Asp) amino acid sequence and PHSRN (Phe-His-Ser-Arg-Asn) synergy sequence. Based on previous crosslinking studies, sequence …
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Kinetics of cell attachment and spreading on hard and soft substrates
… for the functioning of an organism is that cells adapt their behaviour to external stimuli. They continuously interact with their environment, and biochemical and physical cues can activate cellular signalling, which leads to changes in cell behaviour such as proliferation and shape. …
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Analysis of Band 4.1B In Integrin-Mediated Cell Adhesion and Signaling
… adaptor protein that regulates various cellular behavior; however, the mechanisms by which Band 4.1B contributes to intracellular signaling are unclear. This project addresses in vivo and in vitro functions for Band 4.1B in integrin-mediated cell adhesion and signaling. Band 4.1B has …
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Cell attachment to Zein surfaces
… as tissue engineering and circulating cancer cell retrieval. Understanding the mechanism of cell-substrate interaction is pivotal for exploiting the potential of those materials. Adhesion of cells to substrate surfaces is often mediated by tissue transglutaminase (tTG) in its role as a …
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Control of cell fate decisions of embryonic stem cells by mechanical forces
… properties of the substrates, upon which the cells adhere, to influence critical biological functions including cell fate decisions in mesenchymal stem cells. However, how embryonic stem cells respond to forces or underlying substrates is not clear at this time. The work presented here …
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Development of a Novel Single-Cell Attachment and Spreading Platform Utilizing Fused-Fiber Nanonets
Initial attachment to the extracellular matrix (ECM) and consequent spreading is a necessary process in the cell cycle of which little is known. Cell spreading has been well-recognized in 2D systems, however, the native fibrous ECM presents cells with 3D biophysical cues. Thus, using suspended …
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Applications of Traction Force Microscopy in Measuring Adhesion Molecule Dependent Cell Contractility
… controlled elastic modulus substrates for single cell traction force microscopy studies. The first section describes the use of EDC/NHS chemistry to convalently link microbeads to the hydrogel matrix for the purpose of performing long-term traction force studies (7 days). The final study uses the …
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REGULATION OF RHOGAP DLC1 BY FAK, PP2A AND MEK/ERK IN CELL DYNAMICS
… and tumor suppressor, affects the process of cell migration via its regulation of RhoA. A serine-rich multi-domain protein, DLC1 undergoes post-translational modifications such as phosphorylation. It has been identified as a downstream target for various kinases but effects of growth factors, …
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Polymer-tethered epidermal growth factor as an inductive biomaterial surface for connective tissue progenitors
… can act as a pluripotent source of reparative cells during injury and therefore have great potential in regenerative medicine and tissue engineering. However, the response of CTP to most growth factors and cytokines is unknown. Many envisioned applications of CTP, such as treating large defects …
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Produção, caracterização e avaliação in vitro de filmes finos de vidro niobo-fosfato por deposição a laser pulsado
… profilometry, scratch test, focal contact and cell proliferation and differentiation. In XPS analysis, CaNb2(P2O6) and NbOx structures were found. Crystalline phases were not identified on the films, since the glass has an amorphous structure. Together, these results suggest that both material …
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Roles For B-Raf Kinase In The Specific Regulation of Α4Β1 Integrin In T Cells
… Integrins are versatile proteins and mediate T cell migration and trafficking by binding to ECM or other cells, as well as initiating intracellular signaling cascades promoting survival or activation. The mitogen activated-protein kinase (MAPK) pathway is known to be downstream from integrins …
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Electromagnetic stimulation of a composite scaffold for wound healing
… OF MISSOURI-COLUMBIA AT AUTHOR'S REQUEST.] Decellularized tissues have been investigated as natural sources for tissue scaffolds as synthetic mimicry of the natural extracellular matrix is quite difficult. In addition, gold nanomaterials have been studied for their ability to provide …
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Regulation of the Cytoskeleton by Kinesins
… KIF2A and KIF2C are upregulated in cancer cells and the increased protein expression influences cell migration and invasiveness. In order to understand how KIF2A and KIF2C influence migration, we analyzed the microtubule and actin cytoskeleton in cells manipulated for kinesin expression. We …
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Image and video analysis techniques for cellular microscopy
… that can capture dynamic characteristics of sub-cellular and cellular structures. Biologists routinely produce large volumes of bioimage time lapse data that necessitates automated algorithms for unbiased and repeatable quantitative analysis. These algorithms are the stepping stones in bioimage …
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Serine 910 Phosphorylation of Focal Adhesion Kinase Is Critical for Costamere Assembly
… S910A-FAK mutant also blocked ET-1 induced NRVM spreading and cyotoskeletal reorganization. Finally, we found that FAK was serine-phosphorylated at multiple sites in nonfailing, human LV tissue. FAK-S910 phosphorylation and ERK5 expression were dramatically reduced in patients undergoing heart …
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Investigation and development of techniques for the characterisation of the synthetic/biological interface
… in a multidisciplinary research program. Cell adhesion in culture was the primary biological sensor of surface properties, and it showed that the cell response to different materials can be modified by adhesion promoting protein layers: cell adhesion is a protein-mediated event. A range of …
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Nanoscale Organization of The Small Gtpase Rac1
… in impaired macropinocytosis and decreased cell spreading. In this thesis, I proposed that Rac1 nanoclusters act as lipid-based signaling platforms emulating the spatiotemporal organization of Ras proteins and further showed that the Rac1 PBD-prenyl anchor has a biological function that …
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Vascular Network Formation via 3D Printing and Cell-Based Approaches
… bioprinting, and sacrificial templating; and cell-based approaches, in which cellular self-organization processes form vessels; all are deficient in their ability to form a vessel system of sufficient complexity for supporting a large cellular construct. What is missing from the literature is …
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alpha-Actinin-4 and the podocyte: Implications for focal segmental glomerulosclerosis
… result from damage to glomerular epithelial cells called podocytes, a key cell type involved in glomerular filtration. Mutations in the ACTN4 gene, encoding an actin-crosslinking protein, are causative of late-onset familial FSGS in humans. We have developed a mouse model of FSGS by …
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