Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 21 for “"cell specification"”.
-
Molecular analysis of germ cell specification
… the correct differentiation of primordial germ cells (PGCs). PGCs are the first cells in the germline, and their correct specification is essential for the eventual production of gametes (eggs and sperm). Murine PGCs arise as a small population in the post-implantation epiblast in response to …
-
Signalling and transcriptional regulation of human primordial germ cell specification
Primordial germ cells (PGCs), the precursors of gametes, are specified in early post-implantation human embryos, which are inaccessible for direct investigation. This process can however be modelled using human embryonic stem cells (hESCs) for in vitro derivation of human PGC-like cells (hPGCLCs). …
-
CONTROL OF CELL DIVISION AND DIFFERENTIATION IN THE ARABIDOPSIS ROOT - STUDIES ON THE AUXIN RESISTAN 3 (AXR3) GENE
Tight and balanced control of cell division and cell differentiation is crucial for plant development. In Arabidopsis, auxin signaling mediated by its repressors AUX/IAA proteins, plays a key role in mediating root development and growth. However, it is still not well understood how AUX/IAA …
-
The Role of Sonic Hedgehog in the Hypothalamus
… of Shh, such as patterning within a region, cell proliferation, cell specification, and axon guidance. To evaluate this hypothesis, Shh was genetically excised from the hypothalamus using a cre/loxp strategy: Shh brain enhancer 2 (SBE2), with specific activity in the hypothalamus, drove cre …
-
ELUCIDATING REGULATORY NETWORKS PROMOTING B-CELL DEVELOPMENT
B-cells are an essential part of our adaptive immune system. A network of transcription factors together with external signals facilitate the gradual developmental progression from progenitor cells towards the B-cell fate. Several key factors participating in this network have been identified. …
-
Th17 Cell Pathogenicity Promoted by Integrin α3 During Autoimmune Neuroinflammation
… is affected by chronic inflammation, and Th17 cells are critical mediators of disease pathogenesis. While targeting leukocyte trafficking is effective in treating autoimmunity, there are currently no therapeutic interventions that specifically block encephalitogenic Th17 cell migration. Here, …
-
Transformation of human melanocytes and mechanisms of melanoma metastasis
… sites of metastasis exhibited by the melanoma cells created in this manner are analogous to those observed in human patients. The introduction of an identical set of genes into human epithelial and fibroblast cell types results in localized tumor formation in the absence of metastasis. These …
-
Molecular mediators of cardiac-specific enhancer activation
… of how these binding events regulate cell specification. To identify potential key regulatory TFs, we used the Assay for Transposase-Accessible Chromatin (ATAC-seq) to map changes in chromatin accessibility and integrated these data with maps of histone modification patterns and gene …
-
mRNA localization in Caenorhabditis elegans embryogenesis
From guiding cell specification to regulating protein output, post-transcriptional regulation of mRNA is essential for life. As a result, many mechanisms underlying post-transcriptional regulation are highly conserved across the kingdoms of life. As the spatial resolution of microscopy and …
-
The role of notch signaling in the development of the hypothalamic-pituitary axis and arcuate nucleus
… between the two structures to direct their cell specification. However, we do know that ligands and receptors of the Notch signaling pathway, known to control cell specification in other contexts, are found both in the hypothalamus and Rathke’s pouch. Additionally, the Notch signaling …
-
A Mutation in Alk6b Causes Impaired Germ Cell Differentation and Testicular Germ Cell Tumors in Zebrafish
Germ cell tumors (GCTs) affect infants, children and young adults and are increasing in incidence worldwide. GCTs arise from pluripotent germ cells and can exhibit differentiated and undifferentiated histologies, which vary in their malignant potential and response to treatment. The pathways that …
-
Evolutionary and Developmental Survey of Genes Involved in Chordate Pigmentation
… to understand the establishment of eukaryotic cellular organization and for its implication in many human pathologies. For the first time, I reconstructed the evolutionary scenario of Rab family in eleven species of metazoans, spanning from cnidarians to human. Phylogeny, intron code and …
-
Germline development in Platynereis dumerilii and its connection to embryonic patterning
… theory about the evolutionary origin of germ cell specification still requires detailed analyses in other species. In this thesis, I present evidence on the establishment of the germline and its correlation with pluripotency and embryonic patterning in the polychaete Platynereis dumerilii, an …
-
The role of notch signaling in the regulation of proliferation and cell differentiation during embryonic and postnatal pituitary development
… development. During both periods, progenitor cells proliferate and subsequently differentiate into hormone-producing cells. Maintaining the proper number of progenitors and producing the appropriate number and type of endocrine cells is essential to pituitary development and function. Although …
-
The Epigenetic Role of EGR1 during Postnatal Mammalian Brain Development
… mechanism critical for tissue development, cell specification and cellular function. Mammalian brains consist of millions to billions of neurons and glial cells that can be subdivided into many distinct types of cells. We hypothesize that brain methylomes are heterogeneously methylated …
-
Developmental Single-Cell RNA Sequencing in the Sea Urchin Species Lytechinus variegatus and Heliocidaris erythrogramma
<p>The process by which a single cell develops into a complex multicellular organism with specified cell types and well-defined cellular roles is not completely understood, and the evolution of that process is even more enigmatic. In sea urchin embryos, cell fate specification and differentiation …
-
Specification of Stem Cells and Niche During Hair Follicle Morphogenesis
<p>Adult stem cell (SC) behavior is tightly coordinated by the signals received from the "niche" - the microenvironment that the SCs reside in. Little is known about the role of the niche in SC specification during organ morphogenesis. In particular, the question of whether the niche exists prior …
-
Structure-function analysis of the essential islet regulatory factor Nkx2.2
The specification and differentiation of the pancreatic beta cell lineage requires guidance by spatiotemporally regulated signaling cues and a highly orchestrated set of transcription factors. Defining the factors and their regulatory functions that are required for proper beta cell development …
-
Analysis and suppression of mutant sel-12 in Caenorhabditis elegans
… event that releases the LIN-12/Notch intra-cellular domain from the membrane. This step is crucial for nuclear Notch signaling. As a consequence, loss of all presenilin activity, in a sel-12; hop-1 double mutant, causes phenotypes associated with the complete absence of all Notch signaling …
-
Morphogenesis and Female Fate Determination in Vertebrates
… which is made by a population of somatic cells known as the bipotential supporting cell precursors, determines whether an embryo will develop as a phenotypic male or female. Though several molecular pathways have been shown to be required for female fate determination in vertebrates, the …
Page 1 of 2