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Showing 1 to 13 of 13 for “"cell sorter"”.
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세포자멸사 촉진분자를 이용한 암 치료용 단백질치료제의 개발
… blot analysis - 10 G. Fluorescence activated cell sorter(FACS) analysis - 11 H. co-Immunoprecipitation analysis - 11 I. Confocal microscopy - 13 Ⅲ. 결 과 - 14 A. 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분 석 - 14 B. Etoposide 농도별 돌연변이 Bcl-xL(5A) 단백질의 세포자멸사 촉진 효과 분석 - 15 C. 다양한 2차 자극에 대한 세포자멸사 촉진 효과 분석 - …
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Versatile Biological Sample Preparation Platform using Microfluidic Cell Sorting Device
… are often limited by the low purity of target cells/particles due to the presence of significant host background. A spiral inertial microfluidic cell sorting device can be used to separate cells/particles based on their sizes without any specific labeling and result in a purified sample with a …
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Fundamental differentiation and growth characterization of murine embryonic stem cells in varied culture conditions
Although embryonic stem (ES) cells and their pluripotent capability have been elucidated for decades, little study has been done on obtaining the pluripotency profile of ES cells in the incipient stages of differentiation. In this research, an ES cell line with transfected green fluorescent protein …
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Effect of Cellular Positional Identity on Bone Regenerative Capacity for Tissue Engineering
… gene expression also tested. Rat osteoblastic cells from femurs and calvariae were harvested as matched pairs of cultures from 4 male rats. Cells were expanded extensively in medium supplemented with FGF-2, and were shown to maintain their osteoblastic phenotype as characterised by alkaline …
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Studies of the excretion of aluminium by the kidney and the toxic effects of the element on DNA
… effects of Al in vitro on the DNA of pig kidney cell line LLC-PKl were investigated, in an attempt to elucidate some of the mechanisms of toxic action. DNA synthesis was measured using ³H-TdR incorporation. Over increases of both time (9-72 h) and Al concentration (0.01-8.0 mM), ³H-TdR …
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A dielectrophoretic, microfluidic device for sorting lipid-producing organisms used as bio-oil sources
… device for selecting high-lipid-content cells from a heterogeneous population. The device utilizes dielectrophoresis (DEP) to simultaneously sense and sort individual cells based on their internal lipid content. Microorganisms such as yeast, algae, and bacteria produce high fractions of …
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Einfluss von hyperthermen Oberflächen auf Humanblut
… was evaluated using FACS (Fluorescent activated Cell Sorter) for further information. With regard to red blood cells, white blood cells, platelets and hematocrit, no statistically significant temperature-dependent difference could be shown at temperatures up to 45°C. Yet, respecting the …
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High throughput microfluidic technologies for cell separation and single-cell analysis
The heterogeneity of individual cellular behavior in response to physical and chemical stimuli has raised increasing attention in many biological processes. There is great incentive in developing techniques for high throughput single-cell measurements and manipulations. Particularly, cell size has …
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Technologies for the isolation of circulating tumor cells
Metastasis, the spread and growth of tumor cells from the primary site to distant organs, is arguably the most devastating and deadly attribute of cancer, and is ultimately responsible for 90% of cancer-related deaths. Circulating tumor cells (CTCs) are exceedingly rare cells found in the whole …
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Biologic Influence of 900MHz Radiofrequency Identification in Rat
… Hematology analyzer, FACS(Fluorescence activated cell sorter analysis), Hematoxylin-Eosin staining to evaluated the RFID radiation effects. RESULTS: All rats were examined weekly weight. No body weight change was observed in any exposure group. No significant differences in melatonin and serotonin …
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Homing of 111In-Labeled Bone Marrow Mesenchymal Stem Cells in Acute Brain Trauma Model
… bone marrow-derived mesenchymal stem cells (BMSCs) in an acute brain trauma model by 111In-tropolone labeling and to perform the effect of 111In-labeling on the viability and functions of BMSCs. Rat BMSCs were labeled with 37 MBq 111In-tropolone. Their labeling efficiency and in vitro …
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Monozytäre Expression von CD 14 ("Endotoxin-Rezeptor") und proinflammatorische Subpopulationen antigenpräsentierender Leukozyten
… der Durchflusszytometrie (fluorescence-activated cell sorter (FACS))gemessene Expression sog. mCD14-Rezeptoren und der CD14+CD16++-Rezeptoren auf peripheren Blut-Monozyten mit serologischen Parametern wie C-reaktives Protein und Leukozytenzahl. Die Frage, ob die beiden Oberflächenantigene CD14 und …
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The Role of EphA Receptor Signaling in Neuroblast Survival During In Vitro Differentiation
<p>Mouse embryonic stem cells can be differentiated into multiple cell types and can serve as an excellent model for studying developmental processes in vitro. In particular, stem cells can be differentiated into forebrain-like neurons, allowing investigation of nervous system development at single …