Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 15 of 15 for “"cell penetrating peptides (CPPs)"”.
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Transcending the Cell Fate Stereotype: Cell Penetrating Peptide Technology helps Induce Pluripotency in Multiple Cell Types
<p>Induced pluripotent stem cells (iPSCs) are one of the most promising new technologies in the field of regenerative medicine. iPSCs can be generated directly from a patient’s own fibroblast cells then converted into any cell type, thus overcoming challenges associated with embryonic stem cells …
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REGULATED UPTAKE OF BIOPOLYMERS Role of cell surface proteoglycans Implications for drug and gene delivery
Cells continuously export, import, and recycle molecules over the plasma membrane. Internalization, i.e. cellular import of extracellular material, is a fundamental process, which provides cells with nutrients and enables the immune cells of higher organisms to remove debris, sample their …
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Development of a Real-Time Cell Imaging Assay to Characterize the Delivery of a Cell-Penetrating Peptide Adaptor and its Associated Cargo
<p>Cell-penetrating peptides (CPPs) are small peptides capable of transporting molecules across the membrane. In most cases, the membrane is a blockade that prevents molecules from entering the cell, therefore CPPs have attracted much attention. Although CPPs are effective, this system is still …
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Design, Synthesis, and Evaluation of Oleyl-WRH Peptides for siRNA Delivery
… of nucleic acid therapeutics across various cell membranes has always been one of the major challenges. Cell-penetrating peptides (CPPs) containing arginine (R), tryptophan (W), and histidine (H) have been promising for siRNA delivery. Therefore, to enhance siRNA delivery and efficient …
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Investigating Hybrid Cyclic/Linear and Linear Peptides as Vehicles for Nucleic Acid Delivery
… and hydrophilic molecule, which cannot enter the cell through passive diffusion and/or interaction with the cell membrane. Cell-penetrating peptides (CPPs) offer a unique carrier for nucleic acid delivery, where positively charged components of CPP bind to the negatively charged nucleic acid and …
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Utilization of the Cell Penetrating Peptide TAT-CaM in New Biological Systems: Fungal and Bacterial Cell Lines
<p>Cell penetrating peptides (CPPs) are biomolecular vehicles or systems that mediate the delivery of proteins into different cell lines. Since their discovery in the late 1980s, the CPP delivery system has primarily been explored in different types of mammalian cells. The technology has …
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Novel Cell Penetrating Peptides Effect Endosomal Escape and Deliver Protein Cargos into Living Cells
<p>Over the last decade a number of peptides that are rapidly internalized by mammalian cells have been discovered or designed. Cell-penetrating peptides (CPPs) are capable of mediating penetration of the plasma membrane, allowing delivery of macromolecular cargoes to the cell interior. We have …
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USING A NOVEL CELL- PENETRATING PEPTIDE TECHNOLOGY TO INDUCE PLURIPOTENCY
<p>Somatic stem cells have been used as a regenerative medicine tool to treat diseases like leukemia for decades, but they are limited in their ability to self-renew and differentiate. A better alternative, induced pluripotent stem cells (iPSCs), has the ability to generate cells from all three …
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Peptide-mediated delivery of antisense oligonucleotides and chemotherapeutics across biological barriers
Many nucleic acids, peptides, and small molecules struggle to become clinically viable therapeutics as a result of poor delivery. Biological barriers such as the plasma membrane and the blood-brain barrier (BBB) contribute to this challenge as they can limit the passage of macromolecules. …
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Design of Nuclear-Targeting Peptides for Macromolecule Delivery via Machine Learning
… challenge in biomedicine. For example, cell-penetrating peptides (CPPs) are capable of delivering macromolecular cargo to intracellular targets that are otherwise inaccessible. However, design of novel CPPs with high activity and unique structure remains challenging. In this thesis, …
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The Discovery and Chemical Synthesis of Peptides and Proteins that Cross Biological Barriers
… of therapeutic agents to specific tissues or cells remains a complex task. Cell-penetrating peptides (CPPs) and proteins are used as carrier modules, tissue targeting agents, and probes to better understand disease states. In this work, we first investigate CPPs for peptide-mediated delivery …
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Cyclic and Linear Peptides Containing Tryptophan and Arginine Residues as Cell-Penetrating Peptides and Antifungal Agents
… a novel group of linear and cyclic amphiphilic peptides containing different sequences of arginine (R) and tryptophan (W) residues. The peptides included a class of cyclic peptides, [R3W3]2, [R3WRW]2, [RWW]4, [WRR]4, [RWRW3]2, and the corresponding linear peptides, (R3WRW)2, (RWW)4, (WRR)4, …
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Novel cell-penetrating peptide-based vectors for siRNA delivery
… charge of siRNA creates a hurdle for intracellular delivery that has thus far limited its development as a therapeutic. Cell penetrating peptides (CPPs), such as the Antennapedia homeodomain (AntpHD) and its third helix, Penetratin, are well characterised cationic motifs used previously to …
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Synthesis, Characterization, and Evaluation of Amphiphilic Cell-Penetrating Peptides as siRNA Delivery Tools for Cancer Therapy
… the last two decades, it has been found that cell-penetrating peptide (CPP)-based delivery systems show enhanced uptake of siRNA. This dissertation aimed to investigate fatty acyl cell-penetrating peptides (CPPs) as siRNA delivery tools. We hypothesized that conjugation of hydrophobic …
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Characterization of anti-DNA antibody with Tat peptide sequence in CDR
… CDR 교체 방법으로, 제작하고자 하였다. 핵으로 이동하는 세포막 침투성 펩티드 (cell penetrating peptides)로서 가장 잘 알려진, 13개 아미노산으로 구성된 Tat 펩티드 (GRKKRRQRRRPPQ)를 3D8 scFv의 VH-CDR1, VH-CDR2 및 VH-CDR3부위에 교체, 삽입된 scFv 변이체를 클로닝한 후, 단백질을 박테리아세포에서 발현, 정제하였다. 세 종류의 변이체 중에서 수용성 단백질로서의 발현률이 가장 높은 VH-CDR3 부위가 Tat으로 교체된3D8 scFv 변이체 …