Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"cell microenvironment"”.
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Hierarchical cell fluid extracellular matrix interaction in cell microenvironment
… structural interactions between components of cell microenvironment, the extracellular matrix (ECM), cytoplasm, nucleus and fluid, are important phenomena that decide cell level physiological process and tissue engineering applications. One of those tissue engineering modalities is freezing of …
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Cell patterning technology for controlling the stem cell microenvironment
Embryonic stem cells serve as powerful models for the study of development and disease and hold enormous potential for future therapeutics. Yet, over two decades after mouse embryonic stem cells (mESCs) were first isolated, there is still little known about the role of cell-cell signaling in …
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Microfluidic perfusion culture for controlling the stem cell microenvironment
In multicellular organisms, cells do not exist in isolation but communicate with other cells via extracellular signaling molecules, many of which diffuse into the microenvironment. More than most cell types, embryonic stem cells (ESCs) are critically sensitive to their microenvironment, which plays …
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Optimizing alginate-chitosan microcapsules using co-axial air flow method as 3d stem cell microenvironment
Microencapsulation of cells is gaining popular interest in the field of biomedical engineering because it provides a more effective 3D scaffold that can mimic the cell microenvironment. The benefits of using microcapsules are biocompatibility, biodegradability, nontoxicity, and formation under mild …
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Tumor-assoziierte Mikroglia und Makrophagen modellieren durch GPNMB das Tumorwachstum und die Immunzellinfiltration in malignen Gliomen
… role in modulating the immune suppressive microenvironment in glioma. Here, we show that GPNMB is predominantly expressed by TAMs in human glioblastoma multiforme and the murine RCAS-PDGFb high grade glioma model. Loss of GPNMB in the in vivo tumor microenvironment results in significantly …
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A Biphasic Multiscale Investigation of the Biomechanical Microenvironment of Articular Cartilage
… the micromechanical environment of cartilage cells that is thought to regulate cell metabolic activity.The cell microenvironment was studied at six distinct locations using a multiscale post-processing approach. Microscale sub-models were developed with biphasic poroviscoelastic (BPVE) …
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Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
… and microfabrication approaches to manipulate cell microenvironment in culture. To control cell migration and to restrict cell or colony size, cells and proteins were patterned by using molding or printing methods. Poly(ethylene glycol)-based molecules and polysaccharides were used to control …
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Ras Drives Malignancy Through Stem Cell Crosstalk with the Microenvironment
<p>Squamous cell carcinomas (SCCs) are triggered by marked elevation of RAS/MAPK signaling and progression from benign papilloma to invasive malignancy. A subset of tumor-initiating basal progenitors, the cancer stem cells, obtain increased resistance to chemo and immunotherapy along this path. …
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Matrix-mediated formation & innervation of skeletal myotubes
… of tissue engineering utilizes combinations of cells, biomaterials, and therapeutic molecules in order to generate therapies and better understand the emergent behaviors involved in tissue formation and diseases. One of the goals of tissue engineering is to be able to recapitulate the in vivo …
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Progression of chondrocyte signaling responses to mechanical stimulation in 3-D gel culture
… of 3-D chondrocyte cultures increases extracellular matrix (ECM) production and mechanical stiffness in regenerating cartilage. The goal of this study was to examine the progression of chondrocyte signaling responses to mechanical stimulation in 3-D culture during tissue regeneration. To …
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Microfluidic platforms to evaluate the effects of drugs on fertility and proteins on cancer progression – a focus on cisplatin, alpha-smooth muscle actin, and glial cell line-derived neurotrophic factor
… is necessary to get reliable information about cell responses and test drug efficacy and toxicity. Classical cell culture systems facilitate a wide range of clinical in vitro studies. These cell culture systems often consist of static and flat platforms that poorly mimic the conditions in living …
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THE INFLUENCE OF THE MICROENVIRONMENT OF HEALTHY AND TUMORAL CELLS
Cells use the extracellular matrix (ECM), a part of their microenvironment, as a scaffold for their anchorage, and interact with it in a reciprocal way. The ECM is mainly composed of a three-dimensional network of collagen and crosslinked proteins, such as fibronectin and laminin; ECMs from various …
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Cell-to-cell variability and culture conditions during self-renewal reversibly affect subsequent differentiation of mouse embryonic stem cells
Cell-to-cell variability in clonal populations is reflected in a distribution of mRNA and protein levels among individual cells, including those of key transcription factors governing embryonic stem cell (ESC) pluripotency and differentiation. This may be a source of heterogeneity resulting in …
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Three-dimensional Hyaluronan-based Hydrogels Designed for Breast Cancer Spheroid Formation
In vitro culture of cancer cells is traditionally performed with two-dimensional (2D) culture, which fails to model the in vivo tumour cell phenotype and microenvironment. Hyaluronan (HA) is upregulated in breast cancer stroma and thus an excellent starting material in which to culture breast …
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Biofunctional hydrogels for skeletal muscle constructs
… tissue engineering, where common issues such as cell source, material scaffold, bioreactor method or configuration play key roles. Specifically, it is important to translate the existing body of myogenesis knowledge into engineering muscle constructs by examining the impact of the cell …
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Transcriptomic profiling of macrophage polarization
… mice. I also outline recent advances in single-cell RNA-sequencing and some of the most popularly used platforms for single-cell transcriptomics. I then focus on my work characterizing macrophage polarization and repolarization in vitro, where I performed deep transcriptomic profiling at high …
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THE ROLE OF TRANSCRIPTION FACTOR DYNAMICS IN GENE EXPRESSION: DOES TIME MATTER?
… of utmost importance to reveal mechanisms behind cell homeostasis and pathologies. The transcription machinery needs to be perfectly tuned in space and time to control the expression of genes to carry out cellular and physiological processes in the noisy and highly heterogeneous nuclear …
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On the use of process algebra techniques in computational modelling of cancer initiation and development
… data, especially on the molecular and cellular level. Formal modelling is a necessary tool for integrating massive amounts of diverse measurement data into a coherent picture of disease development. Models can be used to test hypotheses about the role of cellular components in system …
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Stem cell membrane engineering: an approach for modulating cell-biomaterial interactions
Cell membrane engineering is an emerging approach in the field of tissue engineering which aims to exploit the modification of cell membranes for a variety of applications including cell homing and retention, stem cell-tethered biosensors, and cell-mediated drug delivery. Compared to procedures …
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The Role of Gata3 in Blood Stem Cell Emergence
The first definitive haematopoietic stem cells (HSCs) produced during embryonic development are generated from a specialised subset of endothelial cells known as haemogenic endothelium. Recently, it was reported that Gata3 plays a dual role in the development of sympathetic nervous system and …
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