Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 95 for “"cell lysis"”.
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Nucleic acid sample preparation using magnetic ionic liquids as cell lysis and DNA extraction solvents
<p>Sample preparation is vital in nucleic acid analysis. Cell lysis is generally the first step in DNA sample preparation where the cell membrane is disrupted to release intracellular components such as DNA. Moreover, the isolation and enrichment of nucleic acids is essential to prevent false …
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Transistor-based biosensing: expanding the functionality of field effect transistors
… novel design of heating/cooling, ultra-localized cell lysis, and electrical detection of nucleic acid amplification using an on-chip electrode, this thesis aims to enable the next generation of portable biosensors for primary care and on-site diagnostics. Chapter 2 presents an overview of the …
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The role of metals in enhanced biological phosphorus removal from wastewater
… grams of phosphorus were released at a 15 d mean cell residence time and 20°C. Approximately 20 mgCOD/L were taken up by the sludge before any phosphorus was released. Phosphorus release could be described by first order kinetics. Phosphorus uptake under aerobic conditions could also be described …
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Investigating the functional roles of the viral frameshift protein 2B* in EMCV infection
… is considered to be a result of the rapid cell lysis caused by EMCV. This highly efficient method of viral release also induces a severe inflammatory response, leading to disease and death. Viral propagation is further heightened by the ability of EMCV proteins to antagonise the host innate …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this hypothesis has not been tested and previous …
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Staphylococcus aureus Panton-Valentine leukocidin (PVL) alters human neutrophil viability and function
… pore-forming toxin with specificity for myeloid cells, including polymorphonuclear leukocytes (PMNs or neutrophils). It is widely assumed that PVL-mediated pore formation in the plasma membrane of PMNs leads ultimately to cytolysis. However, this hypothesis has not been tested and previous …
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Algae Lysis with Pulsed Electric Fields
… [6]. In order to harvest lipids from algae, the cells must first be lysed.</p> <p>Lysing is achieved by breaking the algal cell wall or membrane to separate oil from the rest of the algae biomass. Current lysing procedures use enzymes, pressure homogenization, and/or sonication to lyse cells; …
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Improvement of engineered thermostable xylanase production via immobilization of recombinant escherichia coli onto graphene oxide
… when utilizing E. coli as a host is bacterial cell lysis due to the pressure build-up through overproduction of the expressed recombinant enzyme in the periplasmic space. Thus, the utilization of the immobilization in targeting the recombinant enzyme expression in the culture medium, presents …
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High-throughput multiplexed transcriptome and protein measurements in single cells
We present cell gels, a platform for high-throughput multiplexed measurements of transcriptome and proteome in single cells. This method takes advantage of droplet barcoding and hydrogel chemistry to capture mRNAs and proteins in thousands of cells. We report the challenges of acquiring sequencing …
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Methods for Improving Challenging DNA Profiles and Molecular Preservation of Soft Tissue Samples
… muscle samples were preserved with 96% ethanol, cell lysis solution, or cell lysis solution with 1% sodium azide, which had been stored at room temperature for seven years. Samples were re-extracted to assess the long-term efficacy of these storage solutions. The results show that ethanol and …
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Mechanisms of Adenovirus-Mediated Autophagy
… therapies and apoptosis. Thus other avenues of cell death urgently need to be explored. Autophagy, which is also known as programmed cell death type II, has recently been identified as an alternative mechanism to kill apoptosis- resistant cancer cells. Traditionally, researchers have studied how …
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Mechanisms of Adenovirus-Mediated Autophagy
… therapies and apoptosis. Thus other avenues of cell death urgently need to be explored. Autophagy, which is also known as programmed cell death type II, has recently been identified as an alternative mechanism to kill apoptosis- resistant cancer cells. Traditionally, researchers have studied how …
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Activation State of Human T and NK Cells Increases Sensitivity to Death by Streptococcus pneumoniae
… from patients with pneumonia. When Spn undergoes cell lysis, pneumolysin (PLY) and other intracellular proteins are released. These intracellular proteins allow for Spn to acquire nutrients and to evade the host immune system. PLY is a cholesterol-dependent cytolysin and has been shown to be a …
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Nutrient release from winter cover crops exposed to freeze-thaw events
… cause ice crystals to form in and between plant cells which can, in turn, result in cell lysis and tissue damage, consequently inducing nutrient release. Laboratory and field experiments were done to determine the effect of freeze-thaw conditions on phosphorus release from commonly used cover …
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Development and application of a PCR multiplex to assess the quality and quantity of forensic DNA extracts
… than those typically targeted in forensic analysis. To gain more information on the quality of extracted DNA a new multiplex PCR assay comprising a 4-plex targeting amplicons of 70 base pairs (bp), 194 bp, 305 bp and 384 bp along with two Internal Amplification Contols (IACs) of 90 bp and 410 …
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The isolation and characterisation of MHC-presented peptides from CML-derived cell-lines, with a focus on post-translational modification
… pathways, against which specific CD4+ and CD8+ T cell responses may be generated, study of MHC-presented phosphopeptides may reveal unique cancer antigens with direct links to the neoplastic state. Mild acid cell-surface elution is a rapid and effective method for MHC class-I peptide capture, …
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Screening for kinases regulating subcellular localization of clock proteins using human kinome library
… of human kinome expression library 9 C. Cell culture and transfection 9 D. Fluorescence microscopy 11 E. Quantitative real-time RT-PCR 12 F. Cell lysis 2 G. Western blotting 13 H. Immunoprecipitation (IP) and λphosphatase treatment 14 I. Fly genetics 15 J. Locomotor activity assays …
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A Passive Microfluidic Device for Buffer Transfer of Cells
Buffer transfer of cells is a critical process in many biomedical applications such as dielectrophoresis experiments, optical trapping, and flow cytometry. Existing methods for buffer transfer of cells are time consuming, require skilled technicians and involve expensive equipment such as …
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The interplay between pathogenic bacteria and bacteriophage Chi: New directions in motility and phage-host interactions in Enterobacterales
… II, we describe our discovery of S. enterica cellular components other than motility that are crucial for bacteriophage χ infection, making the key discovery that the AcrABZ-TolC multi-drug efflux system is required for infection to proceed. We additionally found that the host molecular …
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Nonspecific Cell-Mediated Cytotoxicity in the Cat
… assay, naturally occurring cytotoxic cells were demonstrated in peripheral blood and spleens of domestic cats. Lymphocytes from blood and spleen were purified over Ficoll-Hypaque and added to uninfected, vaccinia-infected, or herpes-infected adherent feline origin target cells and to a …
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