Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 9 of 9 for “"cell line development"”.
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Optimisation of CHO Cell Line Development Using Hybrid Modelling for Antibody Therapeutics
… disorders. Chinese Hamster Ovary (CHO) cells are the primary host for mAb production due to their flexible karyotype and human-like post-translational modifications. However, their flexibility poses challenges due to their genetic instability and expression variability upon longer …
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Modeling the effects of advanced automation and process design on Cell Line Development
Research and development of biologic drugs is a time- and resource-intensive process that can span several years and billions of dollars. Any improvements in the efficiency and end-to-end cycle time of this process provide value to producers in the form of reducing at-risk investment in new drug …
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Mammalian Cell Line Development Platform for Recombinant Protein Production: Expanding the Protein Expression Toolbox for Research and Drug Discovery Applications
… of novel therapeutics in our drug discovery pipeline, there is a high demand to produce a variety of recombinant proteins for early-stage drug development projects. In order to equip our protein production process with the tools and capability for diverse protein expression, it is valuable to …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Computation and predictive modeling to increase efficiency and performance in cell line and bioprocess development
A critical early step in the development of a new biopharmaceutical is the selection of the master cell bank. Per FDA requirements, the same master cell bank must be used for all toxicity and clinical trials, as well as all production of the drug should it be commercialized. Developing a master …
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Establishment of a novel Pichia Pastoris host production platform
The Cell Line Development group at Amgen is responsible for manufacturing and optimizing the cell lines utilized in production of Amgen's biologic drug portfolio. Traditionally, these cell lines are produced from mammalian host organisms, primarily Chinese Hamster Ovary (CHO) cells, due to their …
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High-throughput quantification of glycoprotein sialylation
… acid content. The HTM is also required for cell clone screening in cell line development. Current methods for quantifying sialic acid, however, require chromatographic separation that is time consuming and cannot rapidly analyze many samples in parallel. Here we develop a novel HTM for …
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Rationale Entwicklung rekombinanter CHO-Zellen basierend auf Histonanalyse und RNA-Seq: Identifizierung und Verwendung stabiler Integrationsorte
Chinese hamster ovary (CHO) cells represent the most prevalent platform for biopharmaceutical protein production. Random integration of a transgene into the genome during cell line development often leads to a loss of productivity during upstream processing. This is due to loss of gene copies or …