Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"cell growth rate"”.
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Application of MRI and low-field NMR in biopharmaceutical development
… bioprocessing, where mAbs are manufactured by cells, is therefore paramount. To date, nuclear magnetic resonance (NMR) has had limited application in upstream bioprocessing, stemming from the use of high-field NMR systems that require large capital expenditure, specialised facilities, and high …
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Defining the ethanol-stress response in Saccharomyces cerevisiae
… acting as a potent chemical stress on yeast cells. This stress eventually inhibits yeast growth and reduces ceil viability, therefore limiting alcohol concentrations in the final product and increasing fermentation turnover times. The reduced cell growth rate and viability, as well as an …
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
I. INTRODUCTION 1 II. MATERIALS AND METHODS 4 1. Cell cultures, drug treatment and cell growth rate 4 2. Reverse transcription-polymerase chain reaction (RT-PCR) 4 3. Endogenous cellular oxygen consumption rate 5 4. Estimation of intracellular and mitochondrial ROS level 5 5. Total genomic DNA …
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Exploring the Functional Interaction Between CaMK-II and p53
… protein kinases that respond to transient intracellular calcium signaling. CaMK-II has been reported to be involved with transcription regulation, cell motility, neuronal development, cell cycle regulation, and more recently early development of vertebrates (Easley et al., 2008; Rothschild et …
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Influence of Fibroblasts on Metastatic Cancer Cell Drug Resistance in a 3D Microfluidic Cell Array
<p>Modeling an accurate depiction of the tumor microenvironment, (TME), is essential to observe the effect external factors might have on the tumor <em>in vivo.</em> In cancer therapy specifically, the outcomes of clinical treatments are heavily dependent on drug testing methods despite the …
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Measuring single-cell density using serial suspended microchannel resonators
Cells adjust their composition during important physiological processes, including cell cycle progression, apoptosis, and disease. Due to differences in the densities of water and the various macromolecules which compose cells, changes in cellular composition are reflected by changes in cell …
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Validation-based insertional mutagenesis (VBIM) technology identifies adenomatous polypossis coli (APC) like protein (ALP) as a novel negative regulator of NF-κB
… insertional mutagenesis (VBIM) strategy, our lab has identified the novel adenomatous polyposis coli (APC) like protein (ALP) gene as a negative regulator of NF-κB. Preliminary studies from our lab demonstrated that overexpression of ALP led to decreased NF-κB activity by κB …
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Existence and Analysis of a Rotating Stall Inception Continuum & Development of Concept Questions in Fluid Dynamics
… slopes, dψ/dϕ, less than 0.3 have a stall cell growth rate, σ, that agrees with two-dimensional theory. The divergence of stall inception from two-dimensionality is suggested as a distinguishing feature of spike type stall inception compared to modal type stall inception. The second part of …
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The Biogeochemistry of Carbon Isotopes in Local Lakes
… carbon (DIC) pool. This thesis presents two separate, but related studies regarding the influence of biogeochemical processes on carbon isotopes in local lakes. Crystal Lake is a small but deep glacial lake (12.5 acres, 11.9 meters). The study shows that during thermal stratification, the …
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Nervous system cancer : analysis of historical mortality rates in the United States and Japan indicate sudden increases in environmental risk
Nervous System cancer age-specific mortality rates began being recorded for European and Non-European Americans in 1930 and for Japanese in 1952. All ethnic groups show significant historical increases in mortality rates. For the two American data sets, the age- specific pattern for mortality seems …
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Microbial engineering for aldehyde synthesis
… the negative effect of many aldehydes on cellular processes. In this thesis, we investigate several aspects of microbial aldehyde synthesis. Driven first by the hypothesis that targeted gene deletions could decrease endogenous aldehyde reduction in a model E. coli host strain, we …
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A toolset for linking phenotype and gene expression at the single-cell level
The development of single-cell RNA-sequencing has led to a new degree of resolution in the characterization of complex, heterogeneous biological systems. However, existing methods are often limited in their ability to link these whole-transcriptome profiles with complimentary measurements of …
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Characterizing uptake, distribution and fate of CdSe/ZnS quantum dots in Synnechococcus elongatus PCC7942
… the toxicological impact of quantum dots on cells, cell growth rate, membrane destabilization, viability and the activity of photosynthetic pigments were characterized. For characterization of uptake and distribution, flow cytometry, laser scanning confocal microscopy and transmission …
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Chondrocyte Regulation by IL-I and IGF-I: Interconnection Between Anabolic and Catabolic Factors
… and resorption (i.e., metabolism) of extracellular matrix (ECM) by articular chondrocytes (ACs). Two important hormonal regulators of cartilage metabolism are interleukin-1 (IL-1) and insulin-like growth factor-I (IGF-I). These factors have antagonistic effects on chondrocyte activity, and …
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Role of Jak/Stat pathway in the pathogenesis of breast cancer
The Jak/Stat signaling cascade mediates cell proliferation, differentiation, survival, apoptosis and immune responses. Aberrant activation of this pathway mediates neoplastic transformation and abnormal growth of many malignancies including breast cancer, the most common cancer among women, and the …
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Exploration of Cell Cycle-Specific Essential Gene Functions in the Microbial Plant Chlamydomonas Reinhardtii
<p>The cell cycle encompasses all the steps required for cell proliferation, and is normally tightly coupled to growth and division in all organisms. Much research has resulted in a well-supported model of eukaryotic cell cycle control. However, since most of this research has been carried out in …
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Studies of the Kinetics of Cell Cycle Processes in S. Cerevisiae: The Molecular Basis of Start Irreversibility and Cyclin-Cdk Ordering of Mitotic Events
<p>The cell cycle machinery of Saccharomyces cerevisiae consists of a central negative feedback oscillator comprising cyclin-CDK and its antagonist, APCCdc20. This oscillator is stabilized and tuned by positive feedback loops, and its frequency is modulated by checkpoint controls. Either by …
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Detection of mass, growth rate, and stiffness of single breast cancer cells using micromechanical sensors
… stems from a number of different mutations in a cell. These mutations often control the cellular growth and proliferation, a hallmark of cancer, and give rise to many altered biophysical properties. There exists a complex relationship between the behavior of a cell, its physical properties, and …
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Proteomic analysis of Pseudomonas putida F1 during growth on toluene-phenol mixtures
… However. little is known about microbial growth on mixtures, especially in the context of biodegradation. For example, previous research showed that the biodegradation kinetics of Pseudomonas putida F1 growing on mixtures of toluene and phenol were difficult to predict, despite the fact …
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A Multi-level Mechanical Study of Hypocotyl Growth in the Dark
… Arabidopsis thaliana exhibit a striking wave of cell elongation which moves acropetally from the base to the tip of the organ over time. The combined effect of this wave of cell elongation is a pattern of organ elongation. It was aimed to understand the coordination of cellular growth and growth …
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