Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 52 for “"cell fusion"”.
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Significance of Altered Golgi Apparatus and Cell Fusion Events in Ovarian Cancer Cells
… cancer can be partially attributed to cancer cells being highly multinucleated and polyploid in nature. Stress-induced cytotoxic effects of cisplatin - a chemotherapy drug used to treat ovarian cancer, on subcellular organelles including the ER, mitochondria, nucleus and the cytoskeleton are …
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Molecular genetics and functions of Kaposi's sarcoma-associated herpesvirus glycoproteins in viral entry and virus-induced cell fusion
… (BAC) and successfully recovered in 293 cells. The herpesviral glycoproteins are structural components of the KSHV particle and are thought to facilitate virus entry, egress and virus-induced cell fusion. Investigations, described herein, have focused on the genetic manipulation of the …
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Recapitulating Dynamic Disease States from In Vitro to In Vivo: The Pathophysiology of Cell Fusion in Breast Cancer Metastasis and New Model Systems of Intracranial Aneurysm
… three different model systems for studying both cell fusion in breast cancer metastasis and intracranial aneurysms. The bulk of this thesis work focuses on studying intracranial aneurysms. Intracranial Aneurysms (IAs) are weakened bulging points in blood vessels that upon rupture result in a …
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Genetics and functions of the SARS coronavirus spike protein
… spike (S) glycoprotein mediates membrane fusion events during virus entry and virus-induced cell-to-cell fusion. Investigations, described herein, have focused on the genetic manipulation of the SARS-CoV S glycoprotein in order to delineate functional domains within the protein. This was …
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The Role of MicroRNA-378a-5p and its Target Genes in Differentiation and Fusion of BeWo Cells
… in the maternal blood and formed from the fusion of mononucleated cytotrophoblast (CTBs), syncytiotrophoblasts (STBs) are the primary site of numerous placental functions such as exchange of respiratory gases and waste products, transport of nutrients and hormones production. We have …
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Identification of Unique Hematopoietic Stem Cell Properties
Hematopoietic stem cells (HSC) are sufficient and required for life long production of all blood cell lineages. Steady state HSCs are largely quiescent and we demonstrate that the kinetics of HSC cell cycling are slower compared to more committed progenitor cells also during expansion and …
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Contribution of the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein to Host Cell Entry and Membrane Fusion
… to its simple genetic organization, broad host cell tropism, and ability to be easily grown in cell culture. Therefore, VSV has been used as the prototype to study viral entry, transcription, replication, and assembly. Viral entry, a critical step in the lifecycle of the virus, is mediated by …
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Fusion of bovine fibroblasts to mouse embryonic stem cells: a model to study nuclear reprogramming
The cells from the inner cell mass (ICM) of an early embryo have the potential to differentiate into all the different cell types present in an adult organism. Cells from the ICM can be isolated and cultured in vitro, becoming embryonic stem cells (ESCs). ESCs have several properties that make them …
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Hematopoietic stem and progenitor cells and potentials for application in fetal cell replacement therapy
Fetal cell replacement therapy or in utero hematopoietic cell transplantation (IUHCT) is proposed as a non-myeloablative alternative to bone marrow transplantation (BMT) for a number of inborn immunologic, hematologic and metabolic disorders. IUHCT represents the method through which variable …
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DEVELOPMENT OF ANTAGONISTS TARGETING CHEMOKINE RECEPTOR CCR5 AND THE CHEMOKINE RECEPTOR CCR5 – MU OPIOID RECEPTOR HETERODIMER
… integral role within the inflammatory network of cells. Importantly, CCR5 is a mediator in several disease states and can be targeted using small molecule antagonists. Within this work, CCR5’s role in prostate cancer and HIV/AIDS has been exploited in order to develop potential therapeutics and …
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The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization
The cellular and molecular mechanisms specifying the membrane protein composition of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study regulated movement of the …
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OVERT AND LATENT PATHWAYS OF POLARITY SPECIFICATION IN ZYGOTES: THE HAPLOID-TO-DIPLOID TRANSITION
… normal differentiation and function of diverse cell types, from unicellular prokaryotes to multicellular eukaryotes. Polarity specification, the establishment of an axis for directed growth and secretion, allows cells to achieve anasymmetrical state. This phenomenon is conveniently exemplified …
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Syncytiotrophoblast formation is influenced by genes involved in lipid biology
… in the labyrinth, the syncytiotrophoblast (SynT) cells are the key cell type of this region. Multinucleated SynT cells are formed through the process of trophoblast fusion involving a lipid- signalling event and dynamic membrane rearrangement. Defects altering the structure and abundance of SynT …
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Contribution of Secretion Systems to Macrophage Polarization and Cell Death During Burkholderia pseudomallei Infection
… agent of melioidosis. As a facultative intracellular pathogen, B. pseudomallei uses secretion systems as primary virulence factors. The type 3 secretion system (T3SS) is used to escape the phagosome while the type 6 secretion system is responsible for cell-to-cell fusion and multinucleated …
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Identification of Transmembrane and Extracellular Host Proteases that Promote Human CoV Entry and Syncytium Formation
… to occur, the CoV spike (S) protein must mediate fusion between the viral and host membranes. This entry process can also be repurposed during infection to promote cell-to-cell fusion, further contributing to viral spread. To trigger fusion, S must bind its cognate receptor and be cleaved by host …
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Production of Monoclonal Antibodies for Toxoplasma gondii Lactate Dehydrogenase
… of T. gondii, at the first of all, hybridoma cell lines producing monoclonal antibodies with high affinity and specificity against T. gondii LDH (TgLDH) were established. Methods: After 6-8 week old BALB/c mice were immunized with synthetic peptides of TgLDH, splenocytes were harvested. To get …
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Effects of selenite on metabolic events during the proliferation of canine mammary tumor cells
… and nuclear selenium retention in CMT-13 cells was correlated with cell growth inhibition caused by selenite supplementation. Greater quantities of cytosolic selenium-containing proteins and a nuclear selenium-containing protein were detected as the quantity of selenium within CMT-13 cells …
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Multinuclear and Mononuclear Polyploidy in the Drosophila Hindgut and Heart
… organized. Tissues can be composed of many small cells or comparatively fewer large cells that add nuclear content to facilitate tissue growth. The cells can be separate, discrete units or interconnected collectives. The nuclear composition of a tissue has functional consequences from the tissue …
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Custom-Designed Biohybrid Micromotor for Potential Disease Treatment
… improved drug-uptake relying on the somatic-cell fusion ability. The hybrid micromotors containing drug loaded sperm and constructed artificial enhancements can be self-propelled by the sperm flagella and remotely guided and released to the target at high precision by employing weak external …
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