Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 50 for “"cell free DNA"”.
-
Know Thy Cell-Free DNA: Early Detection of Microsatellite Instability Using Ultra-Low-Pass Cell-Free DNA Sequences
Microsatellites are short segments of repeated DNA motifs (i.e., base pair patterns) that are widespread in our genomes. Microsatellites are inherently more mutable than other genomic locations, and since cancer cells undergo many more cell divisions, microsatellites are useful for distinguishing …
-
Cell-Free Dna Sequencing In Multiple Myeloma
<p>Multiple myeloma (MM) is an incurable plasma cell dyscrasia. Recent advances in MM therapy, including CAR-T therapy, have increased survival and shown the value of assessing treatment response with great sensitivity, both in acute and long-term settings. Cell-free DNA, DNA fragments which are …
-
Expanding the accuracy, resolution, and breadth of cell-free DNA investigation
When cells die, they don’t simply vanish without a trace. Instead, they leave behind fingerprints of their genetic and epigenetic identities in the form of cell-free DNA (cfDNA), or the scant amount of highly fragmented DNA circulating in human plasma. As the detritus of apoptotic and necrotic cell …
-
Biological, Clinical, and Functional Implications of Cell-Free DNA Topology in Cancer
Cells can release their DNA into the extracellular space and circulation by active mechanisms or as a product of cell death. In oncology, this cell-free DNA (cfDNA) has emerged as a promising source of cancer biomarkers because it contains actionable information about a patient’s disease and can be …
-
Detecting tumour signals from multi-modal biological features of cell-free DNA fragments
… shallow whole genome sequencing (sWGS) data of cell-free DNA (cfDNA) isolated from plasma. cfDNAs in body fluids inform non-invasive cancer detection. Multimodal Artificial Intelligence (AI) can improve sensitivity by exploiting various biomarkers when cancer signal is sparse. Tumour-informed …
-
A Method for Identification of Pancreatic Cancer through Methylation Signatures in Cell-Free DNA
… throughout the body. Evaluation of circulating cell-free DNA (cfDNA), specifically, detection of circulating tumor DNA (ctDNA), is being explored as an approach for non-invasive ‘liquid biopsy’ that can be deployed widely and cost-effectively to screen for early signs of disease. DNA methylation …
-
Cell Free DNA as a Monitoring Tool in a Long-Term Athlete Monitoring Program
… were to investigate the utility of cf-DNA as a marker of systemic inflammation, fatigue, and training status in a long-term athlete monitoring program (LTAMP). In study one, cf-DNA, other biochemical markers, volume load, and training intensity were measured in weightlifters over 20 …
-
Cell-free DNA and tumor exosome cargo as diagnostic and prognostic marker for prostate cancer
… was aimed to characterize plasma and urinary cfDNA and tumour exosome cargo as diagnostic biomarker for PCa in South African populations with the goal of discovery of reliable, non-invasive, and novel biomarkers of PCa. We performed miRNA sequencing of exosomal RNA extracted from high and low …
-
Patient Understanding of Fetal Sex Versus Gender in the Context of Routine Cell-Free DNA Screening
… of care to screen for fetal aneuploidy using cell-free DNA (cfDNA). NIPT screens for sex chromosome aneuploidies (SCAs) and in doing so, can predict fetal chromosomal sex. Despite sex and gender being distinct concepts, many patients refer to NIPT as “the gender test” and elect testing in …
-
Targeted next generation sequencing to improve solid tumour diagnosis using tissue-derived and cell-free DNA
… (NGS) allows for high throughput sequencing of DNA and is commonly utilised in oncology research. Whilst whole genome and exome sequencing have been used to identify novel mutations in cancer patients, a more rapid and cost-efficient technology is required for clinical practice. Targeted NGS …
-
Dynamics and utility of cell-free DNA release following radiotherapy in prostate cancer and non-small cell lung cancer
INTRODUCTION: The tissue-of-origin of plasma cell-free DNA (cfDNA) can be determined by methylation analysis and circulating tumour DNA identified by next-generation sequencing (NGS). To evaluate the dynamics of cfDNA during radical radiotherapy, total cfDNA was quantified in patients undergoing …
-
Determination of the Feasibility of Using Cell-Free DNA (cfDNA) in the Q-Clamp KRAS Codon Specific Mutation Test
… mutation test experiment was performed using cell-free extracted DNA (cfDNA) instead of FFPE extracted DNA. The results of the experiment showed a clear difference in the Ct’s between the mutant and internal control. Since the delta Ct’s can be discriminated, the assay showed a positive …
-
Qualify Microplate Reader’s Specification and Establish the Acceptance Criteria for Modified PicoGreen Kit in Measuring cell-free DNA Concentration in Clinical Laboratory
<p>Cell-free DNA (cfDNA) is an emerging biomarker that researchers are utilizing to detect diseases in patients, allowing physicians to accurately diagnose and treat patients at the individual level. A few applications that utilize cfDNA to detect diseases in IVD (in-vitro diagnostics) are …
-
Investigating the role of circulating cell-free dna as a mechanistic biomarker in inflammatory bowel disease: development of an integrated precision-medicine enabled platform in Scotland
BACKGROUND: Circulating cell-free DNA (cfDNA) represents a class of biological molecules whose role in inflammation remains poorly understood. Inflammatory bowel disease (IBD), from ulcerative colitis to Crohn’s disease, comprises a spectrum of chronic immune-mediated conditions with complex …
-
Establishing DNA combing to investigate DNA replication stress in vitro
During the mitotic mammalian cell cycle cells faithfully replicate their DNA utilizing multiple DNA replication sites known as origins of replication. DNA is replicated to provide each daughter cell a complete copy of the genome. Replication proceeds bi-directionally from a minority of potential …
-
Investigating the utility of blood borne oncological biomarkers in solid tumours: glioma and melanoma
… work explored the potential utility of plasma cell-free DNA, circulating tumour DNA, cell-free messenger RNA, and bloodborne tumour related proteins as disease biomarkers in patients with glioma and metastatic melanoma. The in vivo research work employed an animal model to study micro RNA …
-
Plasmatische und zelluläre, nicht invasive Biomarker für die Diagnostik kardialer Abstoßungsreaktionen
… the present work was on potential plasmatic and cellular non-invasive biomarkers for cardiac rejection. Analysis of donor-specific cell-free DNA showed a significant correlation between elevated levels and the presence of cardiac rejection and its severity. Furthermore, for the first time, an …
-
Determining patterns of cancer drug resistance from autopsy patients
… level. Additionally, previously collected cell-free DNA samples enable the establishment of both a spatial and temporal picture of cancer drug resistance and progression.
-
Modular CRISPR-diagnostics for Infectious Diseases
… it to the detection of plasma circulating cell-free DNA in tuberculosis, highlighting its potential as a liquid biopsy test for infectious disease management.
Page 1 of 3