Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 36 for “"cell entry"”.
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STRUCTURAL STUDIES ON CELL ENTRY OF RESPIRATORY ENTEROVIRUSES
Enteroviruses (EVs) represent a group of non-enveloped, positive strand RNA
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Evading Innate and Adaptive Immunity During Adenovirus Cell Entry
… (Ad), a non-enveloped, dsDNA virus, enters cells via clathrin-mediated endocytosis. For viral genome delivery to the nucleus, Ad must penetrate endosomal membranes to create defects sufficient for the passage of the 90 nm diameter capsid across cell membranes. Recent observations suggest …
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Systematic analysis of the molecular mechanisms of T cell entry into tissues
It has now become evident that a subset of T cells establish residency in non-lymphoid tissues and function to provide local immune protection and tissue homeostasis. Classically, immunologists have inferred immune function from immune cells sampled from the blood and lymphoid organs, despite the …
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Contribution of the Membrane-Proximal Region of the Vesicular Stomatitis Virus Glycoprotein to Host Cell Entry and Membrane Fusion
… to its simple genetic organization, broad host cell tropism, and ability to be easily grown in cell culture. Therefore, VSV has been used as the prototype to study viral entry, transcription, replication, and assembly. Viral entry, a critical step in the lifecycle of the virus, is mediated by …
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Structural basis for interactions of the Phytophthora sojae RxLR effector Avh5 with phosphatidylinositol 3-phosphate and for host cell entry
… that employ protein effectors that enter host cells to facilitate infection. Plants may overcome infection by recognizing pathogen effectors via intracellular receptors (R proteins) that form part of their defense system. Entry of some effector proteins into plant cells is mediated by conserved …
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Structural and Molecular Basis for Multifunctional Roles of Cellular Proteases
Cellular proteases were first known for their roles in processing peptides such as signal peptides. Recent discoveries have begun to unravel their multifunctional roles in many other biological processes including blood pressure regulation and virus infection. However, the knowledge gathered thus …
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Cofactors in Coronavirus Entry
… important barriers the virus has to cross is the cellular membrane. Enveloped viruses accomplish this task by viral glycoprotein-mediated binding to host cells and fusion of virus and host cell membranes. For the coronaviruses, viral spike (S) proteins execute these cell entry functions. In my …
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Mechanisms of Adenovirus Membrane Permeabilization
… to occur, a virus must first penetrate host cell membranes to access intracellular sites of viral replication. Currently the mechanism through which adenovirus, a non-enveloped, dsDNA virus, disrupts the endosomal membrane during cell entry is not well characterized.</p><p>Recent studies …
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Development of a cargo delivery system and inhibition studies focused on clostridium difficile toxin a
… and cytotoxicity is acheived only after cell entry. This intrinsic mechanism for cell entry is intriguing from research and medical views. Along with a review on existing cargo delivery systems utilizing protein toxins and the usefulness of such a system, here is described the first …
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Understanding the biological barriers to Jurkat Transfection using non-viral peptides
… feasibility of RALA peptide to manufacture CAR-T cells. <br/><br/>As primary T-cells are hard to transfect, the key barriers to the transfection of primary T-cells should be explored for further rational designs of transfection agents. The low level of heparin sulfate proteoglycans on the cell …
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High-throughput profiling of viral class I fusion proteins via mammalian antigen display
… of viral antigens responsible for host cell entry, identification and profiling of vaccine candidates, and elucidation of how viral evolution enables escape from prior immunity and clinical therapeutics. Here I will review our current understanding of viral entry mechanisms, and the …
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High-throughput profiling of viral class I fusion proteins via mammalian antigen display
… of viral antigens responsible for host cell entry, identification and profiling of vaccine candidates, and elucidation of how viral evolution enables escape from prior immunity and clinical therapeutics. Here I will review our current understanding of viral entry mechanisms, and the …
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CHASING VARIANTS: MAKING THE MATCH BETWEEN THE SARS-COV-2 S1/S2 CLEAVAGE SITE AND HOST CELL PROTEASES
… the role of the S protein in fusion and host cell entry. Among these are those mutations around the S1/S2 site, which is thought to be mainly cleaved by furin during virion formation. The 681st amino acid S position in particular has been the site nonynomymous substitutions several times, and …
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Cryo-EM reveals how adeno-associated virus engages its receptor AAVR and an investigation of the GPR108 ectodomain
… and there are still unknown factors in AAV cell entry, uncoating, and host protein interactions. Furthermore, its use in gene therapy can be optimized with more accurate cell targeting and immune evasion. Being a non-enveloped virus, the protein shell of AAV is responsible for all of the …
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Evolution of targets at the host-pathogen interface
… predicted to interact in a wide variety of intracellular signaling pathways. These results provide the basis for further analyses into the structure-function relationship and druggability of SARS-CoV-2 proteins. Chapter 5: Evolution of SARS-CoV-2 cell entry <br>The evolution of molecular mimicry …
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Biopolymer-Hybrid Nanomaterials for Vaccine Formulation
… NPs were administered to human embryonic kidney cells (HEK-293), the pDNA-based formulation performed more efficiently than its mRNA counterpart. However, the resulting pDNA transfection efficacy was only 21 % of the commercial standard Lipofectamine 2000. To improve nucleic acid binding and …
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Plasma membrane-derived vesicles: composition and function
… membrane vesicles, which are released by most cells upon activation with various extracellular stimuli. This study identified that procedures used in the isolation of PMVs could be further improved by first sonicating (sonicating water-bath) samples prior to isolation of PMVs. Following …
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Structural studies of dynein’s interaction with microtubules and herpesvirus
… how herpes viruses hijack dynein. During cell entry, these viruses are transported by dynein over long distances from the cell periphery to the nucleus. The basis of the interaction between dynein and herpes is unknown. I use a combination of cell biology and mass-spectrometry to identify …
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Structural transitions in self-assembled lipid systems driven by induced curvature: from cell-penetrating peptides to programmable vesicles
Arginine rich cell-penetrating peptides are short cationic peptides capable of traversing the plasma membranes of eukaryotic cells. While successful intracellular delivery of many biologically active macromolecules has been accomplished using these peptides, their mechanisms of cell entry are still …
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