Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"cell detachment"”.
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Experimental and computational studies of receptor-mediated cell detachment in shear flow
Specific, receptor-mediated adhesion of cells to ligand-coated surfaces in viscous shear flow is a central phenomenon in many physiological and biotechnological processes. Mechanical strength of specific adhesion is generally presumed to be related to chemical affinity of receptor/ligand bonds, but …
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POLY(N-ISOPROPYL ACRYLAMIDE)-COATED SURFACES: INVESTIGATION OF CYTOTOXICITY WITH MAMMALIAN CELLS AND THE MECHANISM OF CELL DETACHMENT
… temperature range to release intact mammalian cell monolayers capable of being used to engineer tissues. Two factors currently limit the use of cell sheets for this purpose: 1) although the NIPAM monomer is toxic, it is unclear (and highly contested) whether its polymerized form is toxic as …
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Assessment of the biocompatibility, stability, and suitability of novel thermoresponsive films for the rapid generation of cellular constructs
… a slight temperature change: adherent mammalian cells spontaneously release as a confluent cell sheet, which can be harvested for cell sheet engineering purposes. Since its initial use in 1968, many researchers have used pNIPAM to obtain a cell sheet composed of their cell type of interest. The …
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Studying the Role of Mechanical Properties of Cancer Cells in Tumour Detachment, Migration and Secondary Tumour Formation
Tumour cell detachment, migration and invasion, and secondary tumour formation are critical oncogenic processes during cancer metastasis. Although several molecular mechanisms have been reported to facilitate metastasis, it remains elusive how cellular mechanics contribute to these processes. The …
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Synergism of Gingipains in Endothelial Cell Adhesion Disruption and Apoptosis
… the effects of these proteases on endothelial cells, we exposed bovine coronary artery endothelial cells (BCAEC) and human microvascular endothelial cells (HMVEC) to gingipain-active extracellular protein preparations and/or purified gingipains from <em>P. gingivalis. </em>Treated cells …
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Development of a novel micropipette aspiration-based force sensing technique to measure single cell sliding friction
In numerous biological scenarios the motion of cells is tangential to the surface of contact such as in extravasation of leucocytes, blood flow regulation, and cell migration. In these cases, adhesion molecules on the cells form bonds with the substrate upon contact and when the cells propagate, …
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Synthesis and characterization of tethered poly(n-isopropylacrylamide) for quantitative cell adhesion studies
… using ARGET ATRP. A quantitative study of detachment of marine bacteria from these surfaces, both above and below the LCST using a spinning disc apparatus is also reported. This device applies a linear range of reproducible shear forces to cells attached at the interface of a single sample. …
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Functional Investigation of Dual αvβ3 and αllbβ3 Integrin Inhibition in Haematological and Solid Tumour Models
… and flow cytometry in a panel of cell lines. After characterising the expression of αv, αIIb and β3 integrin subunits in inducible and natural expression models (K562 and MCF-7 cells respectively), functional tests for cellular adhesion, detachment and migration were determined. …
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Evaluation of water distribution system monitoring using stochastic dynamic modeling
… between flow velocities and bioflim cell detachment. Seven sampling plans representing regulatory requirements and industry practice were applied during the simulation to evaluate their ability to detect the contamination under a presence/absence criterion. The simulations were able …
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Fabrication, design, and analytical applications of nanostructured plasmonic crystals
… the morphology and dynamic vital activities of cell. Polyelectrolyte layer-by-layer assemblies with well-defined thicknesses were used to calibrate the reflection contrast response as a function of thickness of biomolecular thin film on plasmonic crystal, which was theoretically verified through …
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Development and application of a high throughput cell based assay to identify apoptosis inducing proteins, and functional characterization of the candidate Vacuole Membrane Protein 1 (Vmp1)
… upon overexpression. To achieve this, a cell-based high throughput assay was developed. The assay is based on the detection of activated caspase-3 in cells overexpressing proteins tagged C- and N-terminally with YFP. Apoptotic cells were detected by staining with a specific antibody …
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Mechanisms of cell adhesion regulation by herpes simplex virus
… proteins that extensively remodel the host cell. Three such proteins are pUL21, pUL7 and pUL51. pUL21 is a viral phosphatase adapter. pUL7 and pUL51 form a complex that localises to juxtanuclear membranes and sites of cell-matrix adhesion termed focal adhesions. All three proteins are …
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An in-vitro investigation to determine the neuroinflammatory response of CNS cells to oral bacteria and their virulence factors.
… (IMR32) and the astrocytoma (SGVp12) cell lines and primary mixed glial CNS cells in vitro to virulence factors from Porphyromonas gingivalis ATCC 33277 and 53978 (W50). Methods: Following treatment of each cell line, the morphological responses were examined using …
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Kinetics of cell attachment and spreading on hard and soft substrates
… for the functioning of an organism is that cells adapt their behaviour to external stimuli. They continuously interact with their environment, and biochemical and physical cues can activate cellular signalling, which leads to changes in cell behaviour such as proliferation and shape. …
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Analyzing Interactions Between Cells And Extracellular Matrix By Atomic Force Microscopy
Interactions of cells with the extracellular matrix (ECM) have important roles in various physiological and pathological processes, including tissue morphogenesis during embryonic development, wound healing and tumor invasion. Although most of the proteins involved in cell-ECM interactions have …
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An Investigation into the pharmacology of the ghrelin receptor
… hormone secretagogues, a Flp-In™ T-REx™ HEK293 cell line expressing the GRLN-R was constructed. [35S]GTPyS binding assays confirmed that the GRLN-R was constitutively active through both the Gaq/11 and Gai/o pathways, demonstrated by an increase in [35S]GTPyS loading upon receptor expression …
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Chemomechanics of attached and suspended cells
… coupling in single eukaryotic animal cells is investigated in the con- text of the attached (substratum-adhered) and the suspended (free-floating) states. These dichotomous configurations determine behavioral differences and commonalities relevant to therapeutic reimplantation of stem …
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Materials and Nanostructured Coatings for Soft Tissue Regeneration
… treatment or organ transplantation although cellular therapies based on progenitor cell injection are still problematic. Myocardial Tissue Engineering (MTE) is a Regenerative Medicine approach, able to integrate cell therapy with the use of polymeric substrates (myocardial scaffolds or heart …
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Developing quantitative approaches to determine microbial colonisation and activity in mineral bioleaching and characterisation of acid rock drainage
… submerged in fresh media; beads submerged in cell free leachate; and beads in an unsaturated bed, each in the IMC vial. The highest heat output was measured in the unsaturated bed (263.3 mW m-2 ). The consistency of heat produced by the colonising microorganisms was determined through …