Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 45 for “"cell cycle analysis"”.
-
Budding yeast cell cycle analysis and morphological characterization by automated image analysis
… is a standard model system for analyzing cellular response as it is related to the cell cycle. The analysis of yeast cell cycle is typically done visually or by using flow cytometry. The first of these methods is slow, while the second offers a limited amount of information about the …
-
The anticancer mode of action of the quinone natural product cribrostatin 6
… natural product that induces the death of cancer cell lines in culture, but its mechanism of action and scope of activity are unknown. Quinone based drugs have shown potential in the clinic, making cribrostatin 6 an interesting compound to study. The mode of action of cribrostatin 6 is …
-
Anti-breast cancer activity of Coptidis Rhizoma-Euodiae Fructus formulae
… Coptis-Euodia formulae and standard alkaloids. Cell cycle analysis along with topoisomerase I relaxation assay and topoisomerase II decatanation assay were operated to understand the anti-cancer mechanisms of them. Anti-proliferative activity of Zuo Jin pill (Coptis : Euodia = 6 : 1) against …
-
The Effects of Angiogenic Growth Factors on Mesenchymal Stem Cell Proliferation and Differentiation to Vascular Cell Fates
<p>Mesenchymal stem cells (MSCs) are multipotent cells that can differentiate into several cell lineages, including mural cells, which surround and support blood vessels, and possibly endothelial cells, which form the blood vessel walls. In this study, we investigated the effects of basic …
-
The Function of Phosphatidylinositol 4-Kinase III-Beta in Trypanosoma Brucei
… The protein was exogenously expressed in COS-7 cells and assayed for phosphatidylinositol kinase activity. The expressed protein migrated on SDS-PAGE near the predicted molecular weight of 66 kDa and phosphorylates phosphatidylinositol. Depletion of TbPI4KIII-ß in procyclic T. brucei by RNAi …
-
Sustained Depolarization of the Resting Membrane Potential Regulates Muscle Progenitor Cell Growth and Maintains Stem Cell Identity in vitro
… the myogenic properties of muscle progenitor cells (MPCs) during in vitro expansion for stem cell therapies and tissue engineering applications. Controlling cell fate for biomedical interventions will require insight on all aspects that influence cellular properties. The resting membrane …
-
Metabolic Targeting Of Malignant Glioma: Modulation Of Glycolytic Flux By Erythropoietic Factors
… We have engineered U87-MG glioblastoma tumor cell lines to either over-express (via viral promoter based vectors) or silence (using siRNA inducible vector systems) GATA-1. Cell proliferation was assessed via both MTT assay and cell cycle analysis. Luciferase based reporter gene analysis was …
-
JAG1 Blockade With oHSV Polarizes Macrophages to Create a Unique Vulnerability for Cetuximab-Mediated Senolysis of Glioma Cells
… CAN-3110. Since JAG1 is expressed on both glioma cells and tumor-associated macrophages (TAMs), and its expression correlated with a worse prognosis, we engineered a JAG1-antagonizing oHSV (OncoD-0J1) and interrogated its impact on the tumor and myeloid TME. OncoD-0J1 antagonized JAG1-mediated …
-
Somatic Stem Cell Populations and Studies on the Functional Role and Regulation of ABCG2
… that was identified in breast cancer cells due to its ability to efflux chemotherapeutic drugs. Abcg2 can also efflux Hoechst 33342, resulting in a side population phenotype when stained cells are sorted by FACS. Recent studies have suggested that Abcg2 may be a marker for stem and …
-
Documentation and evaluation of medicinal plants with potential for breast cancer therapy as used by Jakun Community of Kampung Peta, Mersing, Johor
… on the proliferation of MCF-7 and MDA-MB-231 cell lines were evaluated using MTT assay, cell cycle arrest and apoptosis. GC-MS was used to identify active the compounds. Findings showed that A. altilis have the highest antioxidant capacity and M. citrifolia, the least. A. altilis methanol …
-
Exploring the impact of osteoporosis on myogenesis
… the impact of bone factors on skeletal muscle cell differentiation (myogenesis) in the presence of osteoporosis. A series of studies have been completed in mouse models, and our concomitant goal was to expand these studies into humans. Serum used was collected from research subjects in an …
-
Anti-proliferative effect of methyl gallate isolated from mangifera pajang kosterman in selected cancer cell lines
… aromatic ester, methyl gallate which induce the cell cycle arrest and killing mechanism via apoptosis in selected cancer cell line. Chromatography methods were used in this study to isolate methyl gallate (C8H8O5) from methanol crude extract. Identification of the isolated compound was done by …
-
The effects of magnetic field (MFs) on in vitro culture of human umbilical cord derived mesenchymal stem cells (hUC-MSCs)
… increased the proliferation rate of stem cells. Accordingly, this current study aims to investigate the potential impact of induced Static Magnetic Field (SMF) on human Umbilical Cord-Derived Mesenchymal Stem Cells (hUC-MSCs) using Samarium Cobalt (SmCo5). SmCo5 was selected for the study …
-
Investigating a co-operative link between TGF-β and Lyn kinase in Chronic Myeloid Leukaemia
… by uncontrolled proliferation of haematopoietic cells driven by the fusion gene Bcr-Abl. The advent of a mechanism specific kinase inhibitor, Imatinib, targeting the constitutively active Bcr-Abl kinase has paved the way for new treatment strategies. However, resistance emerges, particularly in …
-
Anti-cancer effects and mechanism of actions of aspirin-like drugs in the treatment of gliomas
… of strokes and ischemic diseases. The effects on cell viability, proliferation, apoptosis and migration of aspirin and aspirin derivatives were tested on primary glioblastoma cell cultures, BTNW911 and BTNW 914, and the well-established cell lines, SVG-p12, 1321N1, GOS-3, U87 MG, using the …
-
Dietary regulation of myelopoiesis and stromal cell isolation
… chloride did not alter monocyte, granulocyte, T cell, B cell, or NK cell distributions in blood. In spleen, 10% sodium chloride did reduce the presence of NK cells but had minimal effect on other cell populations. In bone marrow, 10% sodium chloride increased the presence of T cells and both …
-
Stemness status in differentiated and undifferentiated glioma cells
Undifferentiated cancer stem cells (CSCs) with their unique potential of self-renewal and multi-lineage differentiation fuel tumour growth and relapse. Efficacy of glioma therapy can be considerably improved if the target is focused towards successful identification and elimination of CSCs. The aim …
-
Activation of the p53 pathway in combination with photon irradiation for the treatment of neurological tumour cells
… play a critical role in regulating tumour cell proliferation, cell-cycle control, and DNA damage response following irradiation. This study investigated the biological effects of the MDM2 inhibitor AMG232 in combination with photon irradiation in TP53- wild-type and TP53-mutant MB and GB …
-
A toxicological analysis of the cyanotoxin β-methylamino-L-alanine at multiple neural levels and identification of novel modes of activity
… here indicate that BMAA induces increased intracellular Ca2+ influx, DNA damage, mitochondrial activity, lactate dehydrogenase (LDH) release and generation of ROS in human neurons. The amelioration of LDH release in the presence of the N methyl-D-aspartate (NMDA) receptor antagonist MK801 …
-
Synthesis and evaluation of novel antivascular agents for two-photon activation
… anticancer phototherapy. The cytotoxicity and cell cycle analysis of the drug candidates were determined on live mammalian cell lines and candidates were screened for their inhibitory effects on tubulin polymerization; stilbenes with a B ring substitution of a para-carbonyl caused cell cycle …
Page 1 of 3