Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 30 for “"cell culture experiments"”.
-
Mathematical Modeling of Perifusion Cell Culture Experiments
<p>In perifusion cell cultures, the culture medium flows continuously through a chamber containing immobilized cells and the effluent is collected at the end. In our main applications, gonadotropin releasing hormone (GnRH) or oxytocin is introduced into the chamber as the input. They stimulate the …
-
Evaluation of Prototype Cell Delivery Catheters Using Agarose Gel and Cell Culture Experiments
… this mode of treatment. We evaluated prototype cell delivery catheters (each with outer and inner catheter) developed to minimize these complications. The catheters (1.6 mm small bore and 2.0 mm large bore) were evaluated using agarose gel and cell culture experiments. We initially delivered …
-
Biological Adhesion on Nanopatterned Substrates Studied with Force Spectroscopy and Microinterferometry
… were used to investigate integrin-mediated cell adhesion, which is a highly complex biological process and essential for numerous cell functions. With nanopatterns the distance between adjacent single integrin binding sites is precisely defined. Recent cell culture experiments have revealed …
-
Understanding the Effect Of 1,3-Butadiene on Human Lung Fibroblasts: Does Exposure to 1,3-Butadiene Induce Senescence?
Cellular senescence is a state of irreversible growth arrest induced by either telomere shortening (replicative senescence) or telomere-independent signals (stress-induced premature senescence or SIPS). Recent studies have shown that cigarette smoke extract induces senescence in lung fibroblasts …
-
An evolutionary perspective on the sequence, mechanism, and regulatory function of animal microRNAs
… of Hoxb8 were detected in mouse embryos. Cell culture experiments demonstrated down-regulation of Hoxb8, Hoxc8, Hoxd8, and Hoxa7 and supported a cleavage mechanism for miR-196-directed repression of Hoxb8. These results point to a miRNA-mediated mechanism for the posttranscriptional …
-
Development of simple 3D-printed scaffolds for liver tissue engineering
… will require complex structures to support cell differentiation and integration with surrounding vasculature. Recent developments in 3D-printing (3DP™) technology allow the construction of such geometrically complex scaffolds out of biodegradable polymers. These artificial tissues should …
-
Finite element and mechanobiological modelling of vascular devices
… graft performance, mechanical characterisation experiments can be conducted in order to develop constitutive models that can be used in FE models of vascular grafts to predict their mechanical behaviour in-situ. In this context, bacterial cellulose (BC), a novel biomaterial, was mechanically …
-
Macrophage Diversity in the First Trimester Human Placenta: Phenotypes, Functions and Developmental Origins
… of tissue-resident macrophages called Hofbauer cells (HBC), however the functions and developmental origins of these cells remain poorly defined. Our attempts to profile HBC in the first trimester human placenta revealed the presence of maternally-derived cells in placental digests. We …
-
Examining Mechanisms and Functional Significance of Adiponectin Stimulated Autophagy in Cardiometabolic Diseases
… upon low energy status. AMPK activates multiple cellular process to generate energy including autophagy. Autophagy is a conserved catabolic process which confers an adaptive force against stresses by mobilizing energy sources and preventing toxin accumulation. The studies presented here …
-
Self Assembling Bioactive Materials for Cell Adhesion in Tissue Repair
… liquid crystalline phases when cast as a film. Cell culture experiments on these films with 3T3 and 3T6 fibroblasts indicated that these ordered materials form surfaces with specific chemistries that favored cell adhesion, spreading, and proliferation suggesting the potential of mediating human …
-
A Role for Insulin Signaling in Regulating the PTEN Tumour Suppressor in Caenorhabditis Elegans
… neuronal development. I then employed human cell culture experiments and reported that this negative regulation is conserved in human cancer cell lines. I showed that knocking-down IR through siRNA up-regulates PTEN, and over-expressing a gain-of-function IR down-regulates PTEN. I also showed …
-
A mechanism of activation of c-MET receptor tyrosine kinase
… signaling governs numerous important cellular responses including cellular proliferation, differentiation, migration and apoptosis. Deregulation of these signals result in malignant behaviors, often leading to cancers. While the identity of the many signaling molecules that are …
-
Characterization of alginate scaffolds using X-ray imaging techniques
… to smaller pore size and porosity. Secondly, cell culture experiments were carried out to study the biological properties and the interactions of alginate hydrogel with cells. A Schwann cell line was either blended with alginate solution before crosslinking with calcium chloride (CaCl2) or put …
-
Aligned Fibrillar Collagen Matrices for Tissue Engineering
… production of ordered substrates that orient cells preferentially and support cell viability and differentiation. Towards this goal, this thesis investigated methodologies to align extracellular matrix structures in vitro to guide stem/progenitor cell behaviour for tissue regeneration. Aligned …
-
Phylogenetics and New Approaches to Vaccine Development for West Nile
… WNV E glycoprotein specific CD8+CD62Llow IFNã+ T cells response against WNV. Recombinant VSV expressing the Simian Retrovirus-2 (SRV-2) gag and Env gene constructs and the Herpes B virus glycoprotein D gene were constructed and characterized in cell culture experiments. The VSV-SRV-2 gag and env …
-
The Specific Role of the MLL CXXC Domain in MLL Fusion Protein Function
… DNA binding, and by performing domain swap experiments in which different CXXC domains from other proteins, including DNMT1, CGBP and MBD1, replaced the MLL CXXC domain in the context of an oncogenic MLL fusion. DNA binding experiments determined that the MLL CXXC domain has the highest DNA …
-
Mitochondrial mRNA Translation is Required for Maintenance of Oxidative Capacity
… Methods Using a combination of animal and cell culture experiments, we first analyzed the oxidative detriments of the myocardium using an LLC tumor implantation model and followed by assessing how antioxidant protection against LLC-conditioned culture media. Additionally, we used a model of …
-
MicroRNA targeting in mus musculus and Caenorhabditis elegans
… of Hoxb8 transcript in mouse embryos, and cell culture experiments showed down-regulation of Hoxb8, Hoxc8, Hoxd8, and Hoxa7. To address the biological significance of miR-196 mediated repression of Hox genes in vivo, we attempted to generate targeted transgenic mice for the Hoxa7, b8 and c8 …
-
Extracellular Proteoglycan Decorin in Bovine Mammary Physiology
… mammary gland research focuses on the main cell types - mammary epithelial cells and fibroblasts. However, the extracellular matrix (ECM) within the mammary gland is also of importance for its ability to regulate cell shape, proliferation, polarity, differentiation, gene transcription, …
-
The differential Role of Insulin-like Growth Factor-1 Isoforms in Skeletal muscle
… in skeletal muscle in vitro and in vivo. Cell culture experiments revealed that IGF-1Ea isoforms promoted myogenic differentiation and cell hypertrophy, resulting in enlarged myofibers, while IGF-1Eb isoforms instead did not show an effect on fiber size but on proliferation of myoblasts. …
Page 1 of 2