Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 81 for “"cell contact"”.
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Characterization of The Role of The Pkm101-Encoded T4Ss Outer Membrane Core Complex In Substrate Transfer, Pilus Biogenesis, and Recipient Cell Contact
… transfer to recipient plant or bacterial cells, (ii) pilus biogenesis and donor-specific bacteriophage sensitivity, and (iii) recipient cell contact. My results demonstrate that the C-terminal tails of VirB10 and TraF<sub>pKM101</sub> are essential for DNA transfer and pilus biogenesis, …
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Co-culture of hepatocytes with mesenchymal stem cells for cellular therapy in liver disease
… Previous studies showed that mesenchymal stem cells (MSCs) could maintain morphology and improve liver-specific metabolism of co-cultured hepatocytes. The present work aimed to optimise the MSCs co-culture system by testing adipose tissue (AT), bone marrow, and umbilical cord-derived MSCs at …
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Investigating the mechanisms and the temporal regulation of the first cell polarity establishment in the mouse embryo
Embryonic cells of many species polarise and the cell polarity is often important for the normal developmental progression. In the mouse embryo, the prototype of epithelial cell polarity, namely apico-basal polarisation, become established at the 2.5 days’ post-fertilisation, when the embryos are …
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Role of the interaction of proHB-EGF with heparan sulfate proteoglycans
… of the transmembrane form (proHB-EGF) from the cell surface. It was hypothesized that HSPGs interact with proHB-EGF in ways that could alter behavior of the transmembrane form of this ligand and consequent processes. Using an engineered form of proHB-EGF that allowed for independent tracking of …
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Down-Regulation of Natural Killer Cell Activation in Response to Influenza Virus in Older Adults
… vaccine efficacy in elderly people. Type I T cell (Thl)-mediated immunity plays a significant role in Immune responses to influenza infection and vaccination. Natural killer (NK) cells secrete significant amount of IFN-7 , a hallmark Thl cytokine, in response to influenza infection. How aging …
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Role of the Nfa1 Protein in Pathogenic Naegleria fowleri Co-cultured with Target Cells
… of N. fowleri trophozoites against CHO cells, much like treating an anti-Nfa1 antibody on a cocultivating system. In spite of the wide use of N. fowleri in free-living amoebic pathogenicity, no informations what proteins are involved in the functions of these organisms are yet available. …
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Collective migration of epithelial sheets
… of the relationship between motility, cell-cell contact, and their mediation by the mechanical properties of the substratum to which they adhere. Therefore, within this context, this thesis defines the prerequisites for both equilibrium and non-equilibrium coordinated cell motion. The …
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Mechanisms of Eph/ephrin mediated cell-cell communication
… their membrane associated ephrin ligands mediate cell-cell repulsion to guide migrating cells and axons. A peculiarity of this signaling system is that both receptors and ligands can transduce signals into the cell resulting in a bidirectional signaling mode. An important step of ephrinB ligand …
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Structure and function of the R2B adhesive receptor tyrosine phosphatases
… phosphatases (PTPs) are key regulators of cellular signalling through the control of cellular phosphotyrosine levels. The R2B family of receptor-like PTPs – PTPRK, PTPRU, PTPRM and PTPRT - are well placed on the plasma membrane at sites of cell-cell contact to influence cell adhesion. …
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Local Wnt11 Signalling and its role in coordinating cell behaviour in zebrafish embryos
Wnt11 is a key signalling molecule that regulates cell polarity/migration during vertebrate development and also promotes the invasive behaviour of adult cancer cells. It is therefore essential to understand the mechanisms by which Wnt11 signalling regulates cell behaviour. The process of …
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Immune Mechanisms of Mesenchymal Stem Cell Therapy for Acute Graft versus Host Disease
… was to investigate the role of Mesenchymal stem cells (MSC) in the modulation of immune responses, focusing on suppression and induction of immune tolerance. To date, MSC therapy has proved beneficial for the treatment of inflammatory and autoimmune diseases, such as acute Graft versus Host …
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Characterization of Hepatitis C Virus Infection of Hepatocytes and Astrocytes
… of chronic infection. HCV was transmitted via a cell-cell contact-mediated (CCCM) route and in the form of exosomes. Formation of HCV infection foci resulted from CCCM HCV transfer and was cell density-dependent. Moreover, CCCM HCV transfer occurred rapidly, involved all four known HCV receptors …
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Studies of the Hippo Signaling Pathway
How multicellular organisms control their growth to reach proper organ size during development is a fascinating question. Recent studies, initially from Drosophila, have identified the Hpo tumor suppressor pathway as a crucial mechanism that controls tissue growth by inhibiting cell growth, …
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Understanding the Dynamics of Embryonic Stem Cell Differentiation
… defining features of mouse embryonic stem (ES) cells are self-renewal and naive pluripotency, the ability to give rise to all cell lineages in the adult body. In addition to being a unique and interesting cell type, pluripotent ES cells have demonstrated their potential for continued …
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Molecular Interactions of Type III Secretion System Transcriptional Regulators in Pseudomonas aeruginosa: ExsA and ExsD
… potent cytotoxins (effectors) into host cells to suppress the host's innate immune response. Expression of all T3SS-related genes is strictly dependent upon the transcription factor ExsA. Consequently, ExsA and the biological processes that regulate ExsA function are of great biomedical …
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Investigating the role of PTPRK in homeostasis and disease
Cell communication is critical in orchestrating a proportionate response to external factors, such as growth factors and during essential processes, such as junctional remodelling. Plasma membrane-localised receptor PTPs (RPTP) are well positioned to sense external environmental cues, such as …
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Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
… and microfabrication approaches to manipulate cell microenvironment in culture. To control cell migration and to restrict cell or colony size, cells and proteins were patterned by using molding or printing methods. Poly(ethylene glycol)-based molecules and polysaccharides were used to control …
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Synthesis and evaluation of novel kinase inhibitors as anti-cancer agents
… varioustechniques: their ability to restore cell-cell contact growth inhibition in a RhoA-activated cellline; the general cytotoxicity was measured by their ability to inhibit the growth of K562 andA2780 cells; and the effect on the cell cycle analysed using flow cytometry.Three compounds …
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Studies of viral and cellular proteins involved in herpes simplex virus type-1 egress
… a guided pathway that is directed towards the cell adherent points for secretion. Once secreted the vast majority of virions remain associated with the extracellular membrane of cells and very few free virions are released into the culture medium (<1%). The mechanisms that mediate both the …
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Mechanistic Studies of the Roles of the Transcriptional Activator ExsA and Anti-activator Protein ExsD in the Regulation of the Type Three Secretion System in Pseudomonas aeruginosa
… structure used for injecting toxins into a host cell, called the type three secretion system (T3SS). The secretion machinery itself is highly complex and, together with the specific secreted factors, requires expression of more than 30 genes. Due to the high energy cost of its synthesis to the …
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