Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"cationic liposome"”.
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Cationic liposome-mediated transfection of mammary epithelial cells with AAV-based plasmid DNA
Cationic liposome-mediated delivery of adeno-associated virus (AAV) based plasmid DNA has potential for efficient and safe delivery of DNA into a number of different cell types. The objective of this thesis was to develop techniques for transfecting adult somatic mammary epithelial cells with the …
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Catalase-loaded liposomal magnesium phosphate nanoparticles for intracellular protein delivery
… reactions. Previously, our group has developed cationic lipid-coated magnesium phosphate nanoparticle (CAT-LP MgP NP) formulations to enhance the intracellular delivery of the protein, catalase. The objective of the current research is to improve the physicochemical properties of CAT-LP MgP NP. …
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Development of nanoparticle based nicotine vaccines for smoking cessation
… complex to the surface of nano-sized cationic liposome. Significantly higher anti-nicotine antibody titer was achieved in mice by liposome delivered nicotine vaccine compared with nicotine-BSA vaccine. Secondly, a novel nanoparticle (NP)-based delivery platform was constructed by …
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The rational structure-based design of a protein nanoparticle presenting a chimeric MenB antigen
… also examined, demonstrating that an in-house, cationic liposome formulation produced by benchtop microfluidics generated a similar level of immunogenicity as a commercially available MF59-based emulsion adjuvant. Further serological assays were performed to gauge the bactericidal titre …
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The antitumor activity of tumor-targeted RNA replicase-based plasmid DNA
… growth factor (EGF)-conjugated, PEGylated cationic liposome was developed to deliver the RNA replicase-based plasmid, pSIN-beta, into EGFR-over-expressing human breast cancer cells (MDA-MB-468) in vitro and in vivo. Delivery of the pSIN-beta using the EGF receptor-targeted liposome more …
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Nanostructures for protection and vehiculation of bioactive molecules
… (W). The first system investigated is a novel cationic liposome nanocarrier, having interesting performance in topical drug delivery. Both monoolein and lauroylcholine chloride, due to their high biocompatibility, can be regarded as strong penetration enhancers. They are combined to rapidly …
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Developing an in vitro model to study trained immunity
… (GLA), or a novel delivery system containing cationic mannosylated liposome (CL) containing muramyl dipeptide (MDP) and glucopyranosyl lipid adjuvant (GLA). After a 2 days’ rest, cells were restimulated with MDP, LPS, Dukoral or influenza virus for 24 hours. Subsequently, trained immunity was …
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Investigating the role of apoptosis regulator EndoG on exogenous DNA uptake, stability, replication and recombination
… of EndoG was induced by an extrinsic stimulus (a cationic liposome transfection reagent), targeting of EndoG by shRNA improved the perceived stability and transgene expression of pDNA vectors. Therefore, EndoG is not a mediator of exogenous DNA clearance, but in non-physiological circumstances it …
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Untersuchungen zum Aufnahmemechanismus und intrazellulärem Transport von fusogenen und kationischen Liposomen-DNA-Komplexen für den Gentransfer
… elektronenmikroskopischer Methoden verschiedene Liposomen-DNA-Komplexe zum Gentransfer charakterisiert sowie die Aufnahme und Verteilung in der Zellkultur untersucht werden. Dabei waren vor allem solche Präparationen von besonderem Interesse, die in unserer Arbeitsgruppe 'Drug Targeting' getestet …