Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 17 of 17 for “"cationic lipid"”.
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Cationic lipid formaulation of short interference RNA (siRNA) for delivery to respiratory epithelial cells
Cationic lipids are commonly used and relatively safe vectors for plasmid<br/>(pDNA) delivery. In search for an optimal lipid-based formulation for siRNA<br/>delivery to the lungs, cationic lipid-based carriers were investigated for the delivery of siRNA in comparison with pDNA delivery. The aim …
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A novel intracellular protein delivery system - Magnesium phosphate nanoparticles with cationic lipid coating for catalase intracellular delivery
… system - magnesium phosphate nanoparticles with cationic lipid coating for catalase intracellular delivery (LP MgP NP-CAT), and investigate whether it can release the encapsulated catalase to cytosol. LP MgP NP-CAT was designed, prepared and characterized, showing that it had an average diameter …
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Investigation of Phospholipid Bilayer Interactions With Substrates, Polymers, and Nanoparticles
… Bilayers composed of both neat zwitterionic lipid headgroups and cationic lipid headgroups were examined to investigate charge interactions between the lipid headgroups and negatively charged fullerene aggregates. Our results indicate that water soluble fullerenes adhere to lipid headgroups …
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Combinatorial lipid-like materials for intracellular delivery of small RNAs that activate innate antiviral immune responses and adjuvant vaccines
… delivery. A library of structurally-related cationic lipid-like materials, termed "lipidoids", was developed to facilitate uptake of small RNAs, and the role of drug delivery in controlling RNA function was investigated. In an experimental animal model of RNA interference of influenza virus, …
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Catalase-loaded liposomal magnesium phosphate nanoparticles for intracellular protein delivery
… reactions. Previously, our group has developed cationic lipid-coated magnesium phosphate nanoparticle (CAT-LP MgP NP) formulations to enhance the intracellular delivery of the protein, catalase. The objective of the current research is to improve the physicochemical properties of CAT-LP MgP NP. …
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Synthesis, characterization, and evaluation of photo-active amphiphiles for gene delivery applications
… which impede nonviral gene delivery, novel lipids, polymers, and dendrimers are being engineered. Cationic lipid vectors have been widely characterized as gene delivery tools as they electrostatically interact with the anionic nucleic acid backbone to form a supramolecular structure …
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IL -10 gene therapy for the treatment of pulmonary inflammation
… to develop a protocol for in vivo gene transfer. Cationic liposomes represent a class of nonviral vectors that hold great promise as gene delivery vehicles. However, recent studies have shown that they may be associated with some toxicity. We tested the hypothesis that the positive charge of …
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The Impact of Lipid Nanoparticle Chemistry on RNA Delivery and Therapeutic Outcomes
… components of the traditional four-component lipid nanoparticle system, the PEG lipid component and the ionizable cationic lipid component, impact RNA delivery. To systematically investigate how PEG lipid chemistry impacted LNP formulation and RNA delivery, a series of linear-dendritic …
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Lipid Based Frameworks And Topical Ocular Inserts For The Delivery Of Small Molecule Therapeutics To The Posterior Segment Of The Eye
Lipid based systems and topical ocular inserts of various drugs were developed for the intervention/treatment of posterior segment ocular complications. Indomethacin (IN) was developed into solid lipid nanoparticles (SLNs) and Nanostructured lipid carriers (NLCs). Effect of surface …
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Synthesis and conformational study of trans-2-aminocyclohexanol-based pH-triggered molecular switches and their application in gene delivery
… such a switch, when incorporated into cationic liposomes, provides a novel design of the pH-sensitive helper lipids for gene delivery. Protonation of TACH molecules results in a strong intramolecular hydrogen bond between the amino and its neighboring hydroxyl groups, which triggers a …
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Development of Multifunctional Nanoparticles for Cancer Therapy Applications
… prepared by immobilizing a 2 – 3 nm thick phospholipid layer on the surface of superparamagnetic Fe3O4 nanoparticles via high affinity avidin/biotin interactions. Morphological and physicochemical surface properties were assessed using TEM, confocal laser scanning microscopy, differential scanning …
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Dynamische Wechselwirkungen zwischen festkörperunterstützten kationischen Lipidbilayern und oligo-DNA
Das Aufbringen eines Lipidbilayers, den man als artifizielle Zellmembran ansehen kann, auf eine Festkörperoberfläche ist eine häufig genutzte Methode, um ihn mit physikalischen Messmethoden, wie zum Beispiel ATR-FTIR, FRAP, Neutronenstreuung oder wie in der vorliegenden Arbeit NMR, einfacher …
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Using CRISPR/Cas9 to modify the genome of cattle
… sperm. Liposome preparation was done using a cationic lipid, 3-(trimethyl ammonium iodide) 1,2 dimystryl-propanediate (TAID) and a neutral lipid, L- Dioleoyl phosphatidyl-ethanolamine (DOPE) prepared according to given protocol. Percoll or swim-up methods were used to select sperm after …
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Design of Lipid and Polymeric Carriers for Nucleic Acid Delivery
… of the study were to investigate and develop lipid and polymeric carriers for nucleic acid delivery. These included: i) to develop novel cationic lipids for plasmid, oligonucleotide, and siRNA delivery; ii) to develop a novel polymeric delivery system, polyethylene glycol (PEG) based …
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Methoden zur Charakterisierung von großtechnisch hergestellten Lipoplexen zum Gentransfer
Die Instabilität von lipidbasierten Gentransfersystemen und die damit verbundene Schwierigkeit der großtechnischen Herstellung und Lagerung ist ein schwerwiegendes Problem für die Entwicklung und Anwendung gentherapeutischer Arzneimittel. <br>Im Rahmen dieser Arbeit wird eine eingehende …
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Investigating alternative delivery systems for self-Amplifying RNA vaccines
… cell internalisation, SAM was combined with four cationic delivery systems. Oil-in-water cationic nano emulsions (CNE), polymeric nanoparticles (NPs), lipid nanoparticles (SLNs) and liposomes were formulated in the absence of or in combination with a specific SAM vaccine. Despite the differences …
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Tie-ing the endothelium: development of an mRNA-based nanotherapeutic to restore microvascular barrier function for the treatment of sepsis-induced multiple organ failure
… nature, and susceptibility to degradation. Lipid nanoparticles (LNPs) containing ionizable cationic lipids emerged as the most clinically advanced platform for mRNA delivery, but their application for delivery to non-liver tissues is limited. Exploring alternatives to ionizable cationic …