Global ETD Search
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Showing 1 to 4 of 4 for “"catalytic proficiency"”.
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In Search of Catalytic Proficiency: The Importance of Enzyme Conformational Change to Orotidine 5'-Monophosphate Decarboxylase Catalysis
By applying the tools of enzymology learned in the Gerlt Laboratory and working successfully with numerous collaborators, I have furthered our understanding of the mechanism of one of Nature's best catalysts, OMPDC. Increasingly in enzymology, the role of conformational change in enzyme catalysis …
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In search of catalytic proficiency: The importance of enzyme conformational change to orotidine 5’-monophosphate decarboxylase catalysis
The focus of this research is the role of conformational flexibility in catalysis by a TIM-barrel enzyme in pyrimidine biosynthesis, orotidine 5’-monophosphate decarboxylase (OMPDC). OMPDC catalyzes the decarboxylation of OMP to UMP; the uncatalyzed rate for this reaction has been estimated to be …
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Novel function and regulation of mutagenic DNA polymerases in Escherichia coli
… correct nucleotide insertion and an increased catalytic proficiency on the lesion-bearing template relative to undamaged DNA. Moreover, DinB and its mammalian and archaeal orthologs possess similar substrate specificities. Mutation of a single residue in the active site ofE. coli DinB suggests …
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DNA catalysts as phosphatases and as phosphoserine lyases
… and RNA are the only known biopolymers that have catalytic roles in nature, whereas DNA is primarily considered to store and transfer genetic information. However, artificial single-stranded DNA has been identified by in vitro selection to catalyze several chemical reactions and several of those …