Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 84 for “"catalytic mechanism"”.
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Catalytic Mechanism and Maturation of the Metalloenzyme Nitrile Hydratase
… in industry, questions remain about NHase. The catalytic mechanism is not defined. Understanding the way in which the nitrile is converted to amide will be useful for engineering a more efficient, specific, and stable enzyme. The improved enzyme will shift industry towards green chemistry. …
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Structural and biochemical studies of the regulation and catalytic mechanism of ATP synthase
… protrudes into the matrix and it contains the catalytic sites for ATP synthesis. The membrane bound Fo domain contains a proton channel. The two domains are connected by central and peripheral stalks. When F1 is removed from the complex, it can hydrolyse ATP but not synthesise it. It is …
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Hydrogen Production From Model Complexes of the [iron-Iron]- and [nickel-Iron]-Hydrogenase Active Sites
… models for the active site, and neglected the catalytically imperative hydride ligand. Thus, we sought a nickel-iron hydride complex to explore the relevant reactivity of the first (mu-H)Ni(mu-SR) 2Fe complex. We found that the previously reported (dppe)Ni(mu-pdt)Fe(CO) 3, a Ni(I)Fe(I) complex, …
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Engineering Enhanced Cytochromes P450: Understanding Thermostability and Mechanism Through Rational Design and Directed Evolution
… a better understanding of thermostability, catalytic mechanism and substrate specificity was gained.
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Pyruvate Oxidase and the Cytochrome D Terminal Oxidase of Escherichia Coli
… were treated: (i) the active site structure and catalytic mechanism of pyruvate oxidase, (ii) the role of pyruvate oxidase in the E. coli aerobic electron transport system, and (iii) physical characteristics of the cytochrome d terminal oxidase.
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Mechanisms of action and inhibition of [4Fe-4S] proteins IspG and IspH in isoprenoid biosynthesis
… and anti-malarial drug target. However, the catalytic mechanisms of the last two enzymes in this pathway, IspG (also known as GcpE) and IspH (also known as LytB) were largely unknown, and there were no inhibitors targeting these two enzymes. These two enzymes both are [4Fe-4S] proteins with …
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Degradation of atrazine utilizing triazine hydrolase (TrzN) from Arthrobacter aurescens TC1
… for atrazine remediation. However, its catalytic and biochemical properties are not fully understood. This research project provides insight into the catalytic mechanism of TrzN as well as explores the enzymes use in biomaterials for atrazine degradation. Insight into the catalytic …
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Substrate binding to MshB, a zinc-dependent deacetylase in the mycothiol biosynthetic pathway of Mycobacterium tuberculosis
… residues involved in substrate binding and the catalytic mechanism. The findings presented here provide some insight into the substrate binding mechanism and support a recent publication that infers that Tyr-142 plays an important role in the catalytic mechanism, as well as suggesting that VU5 …
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Head-to-head terpene synthases: Mechanism of action and inhibition
… crystallography, and mutagenesis to study the catalytic mechanism of Staphylococcus aureus dehydrosqualene synthase. Dehydrosqualene synthase (CrtM) is also the first committed enzyme in the biosynthesis of staphyloxanthin, a carotenoid pigment, and a major virulence factor in S. aureus. …
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Structural Studies of Enzymes Involved in Drug Resistance and Antibiotic Biosynthesis
… helps to characterize EreB and elucidate its catalytic mechanism, providing a valuable framework to develop efficient inhibitors of EreB for pharmaceutical application.
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Biochemical and Structural Studies of TGT and MiaA: Key Enzymes Involved in Two Types of Hypermodifications
… charged residues. A hypothesis of the MiaA catalytic mechanism is proposed.
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In silico investigation of the mechanism of ricin-catalysed depurination reaction and design of novel ricin inhibitors
… objective of this thesis is to describe the catalytic mechanism of ricin using computational reaction dynamics. For an accurate simulation of the ricin-catalysed reaction, a reasonable model of the target natural substrate is required.
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Structural studies on enzymes involved in uronic acid polysaccharide degradation
… the uronate polysaccharide by a β-elimination mechanism. Polysaccharide lyase enzymes are known to be involved in microbial pathogenicity and gut microbiome interactions. In addition, PL enzymes serve as biotechnology tools for industrial biomass conversion. The overall objective of this PhD …
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The P Cluster of the Azotobacter vinelandii Nitrogenase Complex: Effects of Substitution at the Cluster-bridging Residue, a-Cys88
… nitrogenase component, theMoFe protein, in the catalytic mechanism of biological nitrogen fixation. This dissertation research centers on the role(s) of the P cluster, one of the two unique FeS clusters of the MoFe protein, in the electron transfer mechanism of nitrogenase. Prior to the solution …
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Highly Active Iron-NHC Complexes for Carbonyl Hydrosilylation
The catalytic hydrosilylation of carbonyl groups into alcohols is a powerful transformation in the laboratory and industry. Despite the recent advances in this field, the development of catalytic systems that can activate inexpensive silanes like polymethylhydrosiloxane (PMHS) under mild conditions …
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Mutational studies in the dNTP binding pocket of human immunodeficiency virus type 1 reverse transcriptase
… to understand the function of Gln 91 in the catalytic mechanism, mutants of this residue (Gln91 - -> Ala and Gln91 - ->Asn) were generated and subjected to an in-depth analysis. The efficiency of reverse transcription of natural U5-PBS HIV-1 RNA template was severely impaired by both …
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Structural and Functional Characterization of the Endosome-associated Deubiquitinating Enzyme AMSH
… machinery is a ubiquitin-dependent molecular mechanism made of up of four individual complexes: ESCRT-0, -I, -II, III, that is necessary for regulating the degradation of cell surface receptors directed towards the lysosome. Not only are the ESCRTs implicated in endosomal sorting and …
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Hydrogen production from model complexes of the [FeFe]- and [NiFe]-hydrogenase active sites
… and a wealth of spectroscopic techniques, the mechanism of hydrogen processing remains speculative. Our goals as synthetic chemists have focused on the reactivity of models for the [FeFe]- or [NiFe]- hydrogenases in hopes of understanding of how Nature tunes these first-row transition metals …
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Structural and functional investigations of mechanisms of iron-utilizing enzymes
… Here, I discuss efforts to study the allosteric mechanisms of an essential human iron and free radical utilizing enzyme: ribonucleotide reductase (RNR), and efforts to elucidate the radical based mechanism of isonitrile formation by an mononuclear nonheme iron(II) α-ketoglutarate dependent …
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