Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 12 of 12 for “"catalytic DNA"”.
-
Immobilization of Catalytic DNA for Advanced Materials and Sensors
The DNAzyme was also immobilized on patterned glass surfaces via covalent attachment. Substrate-functionalized 100 nm polystyrene particles were then hybridized in the absence and presence of Pb2+. The result was the Pb2+-mediated tethering of particles onto patterned grids, where Pb2+ induced the …
-
Exploring the Structural and Functional Characteristics of a Catalytic DNA
Finally, the use of DNAzymes in biosensing applications was studied for Pb2+ detection. The molecular beacon DNAzyme biosensor was shown to be capable of detecting Pb2+ in soft to moderately hard water sources when used in conjunction with a handheld fluorometer. Detection limits under these …
-
In Vitro Selection and Characterization of Catalytic DNA in Model Biological Systems
… to obtain useful information from heterogeneous DNA sequencing data. A sample of the selection population from each of the twelve rounds of in vitro selection was heterogeneously sequenced without any prior separation or purification. The observed concurrence of qualitative changes in the …
-
The in vitro selection and biochemical characterization of metalloDNAzymes
DNAzymes are strands of catalytic DNA. First discovered in 1994, they have proved themselves capable of catalyzing many different types of reactions with significant rate enhancements. Because they often require divalent metal-ion cofactors, DNAzymes have readily been developed into metal-ion …
-
DNA as a Catalyst and as a Sensor: Investigating the Structure of the Ty1 Retrotransposition Intermediate and the Utility of Dna-Based Biosensors
The catalytic and binding properties of functional nucleic acids have been exploited to investigate key biochemical questions and to detect small molecules for diagnostic applications. Catalytic DNA (deoxyribozymes) can catalyze many different chemical reactions such as RNA ligation, RNA cleavage, …
-
Fluorescent and Colorimetric Biosensors Based on DNAzymes and DNA Aptamers
… of functional nucleic acids, a large number of catalytic DNA (DNAzymes) and aptamers have been isolated, many of which possess high analyte specificity. As a result, these functional nucleic acids can be used for sensing and diagnostic applications. However, the number of successful sensors made …
-
Dynamics of human DNA Topoisomerases I and II
… tool for investigating the cell biology of human DNA topoisomerases. Our most important finding was, that both types of mammalian topoisomerases are entirely mobile proteins that are in continuous and rapid flux between all compartments of the nucleus and between the cytososl and the chromosomes …
-
Characterization and design of hydrogen bonding interactions in oxygen reduction by engineered myoglobins
… the first crystal structure of a metal mediated catalytic DNA construct."
-
Development of functional DNA-based sensors and investigations into their mechanism
… to a new paradigm in nucleic acid chemistry. Catalytic DNA molecules also known as deoxyribozymes or DNAzymes were first isolated in 1994 through an in vitro selection procedure and have since been engineered and isolated to perform various functions that include both RNA and DNA cleavage and …
-
In vitro selection of deoxyribozymes for O-glycoside cleavage and for 3-nitrotyrosine modification
… and perform catalysis in nature. Single-stranded DNA is structurally similar to RNA and is also capable of catalytic function. DNA enzymes, or deoxyribozymes, are not known to exist in nature, but they have been identified in lab by in vitro selection for a variety of chemical reactions. De novo …
-
I. In vitro selection of aptamers for perchlorate and melamine II. Towards crystallization of DNAzymes III. A highly selective lead sensor based on a classic lead DNAzyme
… 1994, another class of functional nucleic acids, catalytic DNA (DNAzyme) has been isolated using a similar in vitro technique (in vitro selection) to catalyze the cleavage of a phosphodiester bond. Similar in principle, both SELEX and in vitro selection are combinatorial chemistry technique that …
-
In vitro selection and characterization of mono-, di-, and trivalent metal-dependent DNAzymes and their sensing applications
… ligands with high affinity and selectivity. Catalytic DNA or DNAzymes, another class of functional nucleic acids, were first isolated from pools of random DNA in 1994 using the in vitro selection procedure to catalyze the cleavage of a phosphodiester bond. Since the discovery of the first …