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Showing 1 to 9 of 9 for “"carbonic anhydrases"”.
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Design of Nanostructured Peptoid Assemblies as Synthetic Analogues of Natural Carbonic Anhydrases
… solution to the reduction of atmospheric CO2 and carbonic anhydrase functions as a natural carbon dioxide hydrase to hydrate carbon dioxide efficiently. However, carbonic anhydrase (CA) encounters multiple extraction and preservation difficulties and can easily become inactivated. Thus, …
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Design and Synthesis of Nanostructured Peptoids Assemblies that Mimic Natural Carbonic Anhydrases
Artificial Carbonic Anhydrase (CA) mimics that resemble natural CA have gained increasing attention recently due to their low-cost synthesis, high tunability, and high efficiency. However, it remains a significant challenge to precisely control nanostructure-based CA mimics and achieve high …
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Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii
… (CCM). Important components of the CCM are carbonic anhydrases (CAs), zinc metalloenzymes that catalyze the interconversion of carbon dioxide and bicarbonate. C. reinhardtii now has nine carbonic anhydrases, three alpha-CAs and six beta-CAs. This dissertation describes the identification and …
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Carbonic anhydrase from Methanosarcina thermophila: proposal of a new class of carbonic anhydrases and putative roles for the enzyme in anaerobic acetate catabolism
Carbonic anhydrase (CA) from acetate-grown Methanosarcina thermophila strain TM-1 was purified> 10,OOO-fold (22% recovery) to apparent homogeneity and a specific activity of 4,900 units mg⁻¹.The gene encoding this CA was isolated fronl a partial genomic library on a 12-kb fragment and sequenced. …
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Production of biofuels and biodegradable plastics from common waste substrates in engineered Ralstonia eutropha
… pH and ion transport. Four R. eutropha carbonic anhydrases were identified to play individual and non-complementary roles in CO₂ metabolism. An extracellular lipase and its chaperone were identified and characterized in R. eutropha. This lipase is crucial for growth on plant oil and when …
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Role of the small subunit of Rubisco in the green algal phylogeny and Carbon Concentrating Mechanism expression
… carbon transporters and strategically located carbonic anhydrases, elevated CO2 within the pyrenoid improves photosynthetic efficiency. Most photosynthetic organisms have an hexadecameric Rubisco holoenzyme (L8S8), composed of eight ~55-kDa large subunit (LSU), encoded by a chloroplast gene …
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Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii
… active inorganic carbon transport systems, carbonic anhydrases and the localisation of Rubisco to a central chloroplast microcompartment called the pyrenoid. In addition to changes in gene expression, acclimation to low CO2 is accompanied by alterations to metabolism, physiology and even …
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Untersuchungen zur Bedeutung der Carboanhydrasen CahA und IcfA für die Adaptation von Helicobacter pylori an das Extremhabitat Magen
Durch Mutagenisierung erfolgte im Helicobacter-Stamm 26695 eine Ausschaltung der Gene für die Carboanhydrasen CahA und IcfA. Während der Verlust einer Carboanhydrase zu keinem Wachstumsnachteil führte erwies sich der Verlust beider Enzyme für das Bakterium als letal. In nachfolgenden …
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Charakterisierung der Carboanhydrasen von Helicobacter pylori : Funktionen in der Säureresistenz und im Bicarbonat-Metabolismus
In den Helicobacter pylori-Stämmen 26695 und 1061 erfolgte durch Mutagenisierung die Ausschaltung der Gene für die beiden Carboanhydrasen CahA und IcfA. Die Carboanhydrasen zeigten sich im einzelnen mutagenisierbar, Doppelmutationen waren jedoch letal, was die Bedeutung dieser Enzyme für H. pylori …