Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 56 for “"carbodiimide"”.
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Chemical modification of catalytically essential functional groups in the active site of papain
… and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide did indeed inactivate papain. Inactivation by N-ethoxycarbony1-2-ethoxy-1,2-dihydroquinoline appears to be the result of reaction of this reagent with the free thiol group of the enzyme. Treatment of papain with …
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Covalent Attachment of Coordinatively-Saturated Metal Complexes to Horse Cytochrome-C Lysines
… covalently attached to horse cytochrome c via a carbodiimide-promoted condensation of the pendant carboxylate of the complexes with the $\varepsilon$-amine of lysine to form an amide-bond linkage.
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I. Efforts Toward the Total Synthesis of (-)-Crambidine. II. Reactions of Azy(3-Me)-Containing Peptides With Thiols and Selenols
… a thioimidate and an electron deficient vinyl carbodiimide, has been investigated. This approach has resulted in several advanced intermediates related to (-)-crambidine, but an efficient total synthesis of (-)-crambidine has not yet been completed. Additionally, an investigation into the …
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Architectural, mechanical and biochemical control of collagen films and scaffolds to guide neural- and fibroblast cell behaviour
… the stiffness from 10s of MPa to 100s of kPa. Carbodiimide cross linking using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC) in the presence of an N-hydroxy-succinimide (NHS) catalyst resulted in dose-dependent stiffness. Cross linking was varied from 0% to 100% (defined as …
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Development of a Diastereoselective [4+2]-Annulation of Vinyl Carbodiimides With Chiral N-Alkyl Imines and Its Application to the Synthesis of the Batzelladine Alkaloids: Total Synthesis of (+)-Batzelladine a and (-)-Batzelladine D
A diastereoselective [4+2] annulation of vinyl carbodiimides with chiral N-alkyl imines has been developed to access the stereochemically rich anti-fused polycyclic guanidine cores of the batzelladine alkaloids. Application of this strategy, together with additional key steps such as a long-range …
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Fabrication of DNA microarrays on Poly (methylmethacrylate) substrates for biomolecular reporting
… oligonucleotide probes onto these surfaces via carbodiimide coupling chemistries. The photo-modification process for microarray fabrication involves only three steps; (1) broadband UV exposure of the polymer surface; (2) carbodiimide coupling of amine-terminated oligonucleotide probes to the …
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Structural characterization of polyurethane foam and implications of aging
… the change in intensities of diisocyanate, carbodiimide, and uretoneimine bands in the IR spectra. Thermal analysis (DSC and TMA) were consistent with IR finding, where endothermic and exothermic events could be associated with chemical changes in the foam.
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Silica based immunoassays for a covalently attached antigen
… on the modified slides and beads were used for carbodiimide mediated coupling to carboxyl groups on a peptide antigen. High surface concentrations of antigen were achieved. Antibody binding to the antigen-modified beads and slides was measured using an iodinated monoclonal antibody. The …
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Preparation of Pyridinium and Diaminocarbonium Barbituric Acid Ylides
… of the reaction of barbituric acid with carbodiimide, a general synthetic procedure for the preparation of 5-diaminomethylenebarbiturates (DABA) was developed. This procedure is very simple and applicable to the preparation of large quantities of DABA derivatives. Through the X-ray …
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New Derivatization Strategies for Installation of Fixed, Permanent Charges onto Analytes of Biological and Environmental Concern
… C-alkylation with 6-bromohexanoic acid via carbodiimide coupling, reduction and amine alkylation produced a MAD+ in generous yields. The reaction of this MAD+ with 3,5-di-tert-butylphenol demonstrated its ability to induce its fixed permanent charge onto analytes, allowing its detection in …
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Determination of Pancreatic and Salivary Amylase By Enzyme Immunoassay and Their Prevalence in Hyperamylasemic Patients
… was coupled to bovine serum albumin (BSA) by carbodiimide coupling. Monoclonal antibodies were produced against p-amylase and s-amylase (PAb and SAb). These were purified by Protein-A affinity chromatography and then coupled to horseradish peroxidase (HRPO) by carbodiimide coupling. The …
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The performance of cross-linked acellular arterial scaffolds as vascular grafts; pre-clinical testing in direct and isolation loop circulatory models
… with pentagalloyl glucose and collagen with carbodiimide/activated heparin and implanted them as trans- position grafts in the abdominal aorta of rats as direct implants and separately as indirect, isolation-loop implants. All implants resulted in high patency and animal survival rates, …
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Mild [Pd(OAc)2/PPh3] catalyzed cyclization reactions of N-Alkyl-2-vinylazetidines with heterocumulenes.
… acquired in reactions with isocyanates, diarylcarbodiimides, and arylisothiocyanates. Unoptimized results in reactions with ketenes and ketenimines indicate a variety of carbon electrophiles could be used for the insertion and suggest great potential for these methods in organic synthesis. The …
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A comparative study of common crosslinking agents for electrospun collagen scaffolds
… genipin, or Dimethyl aminopropyl)-N'-ethyl carbodiimide with and without N-hydroxysulfosuccinimide. The fibers were characterized using both chemical and physical tests to compare the effectiveness of different crosslinker and crosslinking concentrations. The tests involved mechanical …
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Behavior of passaged chondrocytes in collagen-glycosaminoglycan scaffolds : effects of cross-linking, mechanical loading, and genetic modification of the scaffold
… irradiation (UV) < gluteraldehyde (GTA) < carbodiimide (EDAC). EDAC scaffolds also provided for the highest levels of cell proliferation and protein and GAG synthesis throughout a 4-week culture. Static mechanical compression (0-50% strain) applied to cell-seeded EDAC cross-linked scaffolds …
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Design and Analysis of a Collagenous Anterior Cruciate Ligament Replacement
… radiation and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) were investigated as possible crosslinking methods for the scaffolds, and EDC crosslinking was deemed more appropriate given the gains in strength and stiffness afforded to individual collagen fibers. Scaffolds were composed of 54 …
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Synthesis and Cytotoxicity Studies of Gold Nanoparticle Systems
… functionalisation strategies such as carbodiimide coupling, biotin-avidin interaction and Ni-NTA-histidine interactions following the introduction of the bivalent hexadentate Ni(II) onto the NTA matrix of the MPCs, were conducted to formulate the biomolecular hybrid systems. A range of …
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From Micro- to Nano-Scale: Applications of Solid-Phase Enzymatic Reactors for Biopolymer Disassembly
… accomplished using 3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling to carboxylic acid functionalized PMMA micropillars. Our micro-scale results suggest that the reaction efficiency for the catalysis of dsDNA digestion using λ-exonuclease, including its …
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Gradients in Collagen Films: Effects of Mechanical and Chemical Properties on Directional Cell Response
… The use of 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride with N-hydroxysuccinimide (EDC/NHS) influenced both the mechanical and chemical properties, while crosslinking using genipin altered mechanical behaviour without changing surface chemistry. By adjusting the rate of …
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