Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"capsid proteins"”.
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Genetic and Biochemical Analyses of the Flaviviridae Capsid Proteins
… particles, thought to arise by budding of the capsid protein and associated genomic RNA through a host cell-derived lipid membrane outfitted with envelope glycoproteins. The process of virion morphogenesis is not well understood, but the presumed involvement of numerous viral and cellular …
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MUTAGENESIS OF AAV CAPSID PROTEINS FOR ENHANCED TRANSDUCTION OF CARDIAC CELL TYPES
… surface domains of AAV9 and chimeric AAV9-2 capsids. Mutations in the VP3 capsid sequences, cloned into helper plasmids pXR9 and chimeric pXR9-2 (substituting the 3-fold loop region of AAV9's VP3 with AAV2's), were used to generate a small library of rAAV9 or chimeric 9-2 vectors with mutant …
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A study of the kinetics and mechanism of inactivation of a DNA- containing enteric virus by chlorine
… action of chlorine on parvovirus H-1 was on the capsid. Alterations in the two major capsid proteins caused the virion to rupture, and, as evidenced by electron microscopy the ssDNA was exposed. Also, the adsorption ability of the chlorine-treated virions to host cells was significantly …
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Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
… library was screened to identify aphid proteins that interact with the BYDV-PAV-IL capsid proteins using the yeast two-hybrid system. Seven clones activated the expression of the reporter genes, supposedly by interacting with capsid proteins of BYDV-PAV, but complementation tests …
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Production and immunogenicity of chimaeric human papillomavirus-like particle vaccines
… -16 and -18. Both vaccines are based on the L1 capsid proteins of the types they are targeted to and are efficient, pro- phylactic, typespecific vaccines. However, two problems remain: they do not protect against nonvaccine types, that may cause a significant proportion of cancers specifically …
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Novel expression and production of Foot-and-mouth disease virus vaccine candidates in Nicotiana benthamiana
… VLPs are formed by the assembly of the FMDV capsid proteins VP0, VP1 and VP3, which are generated upon the proteolytic cleavage of the capsid precursor protein P1-2A, by the FMDV 3C-protease. The expression platforms used to co-express and produce the component capsid proteins and the …
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Analysis of factors that affect parvovirus expression
… active in temporal order during infection. BPV proteins also appear in the same temporal order; the nonstructural (NS) proteins are produced before the capsid proteins. Northern blot analysis of BPV RNAs suggest that, like human parvovirus B19, all transcripts of BPV are initiated from a single …
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The association of bovine parvovirus DNA and proteins with the nuclear matrix of infected cells
… the nonstructural BPV protein, NP-1, and the capsid proteins VP1, VP2, and VP3 were associated with the matrix at sixteen hours. The association of BPV DNA and proteins with the nuclear matrix implies structural if not functional significance for the matrix in BPV replication.
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New Biological Pathways
… retrieval, I propose repurposing viral capsid proteins to perform non-destructive transcriptomic measurements. We demonstrate that this approach allows for live-cell transcriptomics, and we longitudinally measure the transcriptional responses of the same living human cells after …
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Engineering M13 bacteriophage platforms for cancer therapy applications
… of small viral ssDNA that are packaged by M13 capsid proteins. These 'inho' phages can be sized to ~50nm and above in length. The small phage retains the M13 major and minor coat proteins which have previously been manipulated to serve as tethers to carry various therapy and imaging agents and …
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Optimization and characterisation of plant produced Human Papillomavirus pseudovirions in Nicotiana benthamiana
… are made up of the L1 major and L2 minor HPV capsid proteins and encapsidate up to 8Kb of pseudogenome DNA without the need of an encapsidation signal. HPV PsVs are used to test neutralising antibodies elicited by vaccines, for studying the virus life cycle, and potentially for delivery of …
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Towards the development of plant-made PsVs as potential delivery vehicles for therapeutic HPV vaccines
… viral particles consisting of L1 and L2 capsid proteins, which self-assemble to package pseudogenome DNA. Pre-existing immunity to high-risk HPVs through natural infection and vaccination, however, preclude their use as delivery vehicles for DNA vaccines. The development of non-human …
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The use of transgenic tobacco as a production and delivery system for a vaccine against hemorrhagic enteritis virus of turkeys
… bacterial infections. Data indicates that the capsid proteins (fiber, penton base, hexon) of HEV are capable of stimulating protective antibodies against an HEV challenge. Using tobacco as a model, we sought to determine if a plant could be used to synthesize the HEV fiber protein and produce …
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Ancient Immune Systems: Sensing and Signaling in Bacterial-Phage Conflict
… system in bacteria, detects specific phage capsid proteins [4]. This recognition event triggers the production of a signaling molecule that activates the depletion of an essential metabolite, limiting viral propagation. The second discovery demonstrated that cyclic oligonucleotide-based …
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Molecular Study of the B19 (Human) Pathogenic Parvovirus
… overlapping transcripts of B19 produce two capsid proteins, namely VP1 and VP2, where VP1 is the major protein produced. Immediately upstream from the VP1 translation site is an unusual sequence containing multiple ATG codons. During processing, this sequence is spliced out of VP2 RNA. A set …
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Inactivation mechanisms of human norovirus surrogate Tulane virus by peracetic acid at different pHs
… by the damage of the genome or the protein capsid or both. The genome damage was revealed by quantifying the viral intact genome using the reverse transcription real-time quantitative polymerase chain reaction (RT-qPCR). The capsid proteins degradation is determined by quantifying the …
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Increased Apoptosis in the Host By Guanidine Hydrochloride Treatment of Infectious Pancreatic Necrosis Virus
… apoptosis within their host. Particles or empty capsids that produce inefficient or no protein products will allow host cells to undergo apoptosis. Empty capsids are mass produced by some viruses or can be created from wild type virus by nucleic acid extrusion. This process has been done with …
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Development of plant-produced Bluetongue virus vaccines
… cells by the co-expression of the four BTV capsid proteins (VP2, VP3, VP5 and VP7). Sheep vaccinated with insect cell-produced BTV VLPs were shown to be protected against challenge with wild type virus. However, the high costs associated with the production and scale-up of BTV VLPs in insect …
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Mechanisms of phage detection by bacterial innate immune proteins
… directly binding to the newly synthesized major capsid proteins (MCPs) of certain phages. Binding to the MCPs releases autoinhibition of the CapRel_SJ46 toxin domain, enabling it to pyrophosphorylate tRNAs, which blocks translation to restrict viral infection. Detection of the MCPs is analogous …
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