Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"capsid gene"”.
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The Multifunctional Capsid Gene of Hepatitis C Virus
… Hepatitis C Virus displays great diversity. The capsid gene, however, is remarkably conserved and this has led to predictions of RNA secondary structure and an overlapping, alternative reading frame (ARF). We investigated a role of the ARF in HCV infection, by introducing four stop codons into …
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HEPATITIS A VIRUS: GROWTH CHARACTERISTICS, PURIFICATION, AND CAPSID GENE ORDER (PEPTIDES, IMMUNOREACTIVITIES, POLYPEPTIDES).
… potential immunogenic regions within the HAV capsid polypeptides. Synthetic peptides corresponding to selected regions of each of the larger putative capsid polypeptides were coupled to keyhole limpet hemocyanin and used to immunize rabbits. EITB reactivities of HAV specific anti-peptide sera …
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An investigation into the high level production of proteins in tobacco using transgenic plants or viral vectors
… The chloramphenicol acetyl transferase gene (CAT) was inserted between the two UTRs. This artificial viral cDNA (5'cat3') was cloned immediately downstream of the cauliflower mosaic virus 35 S promoter at the transcription initiation site to make a DNA vector. An RNA vector construct was …
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Molecular Breeding of Porcine Circovirus Type 2 by Synthetic DNA Shuffling
… proteins and ORF2 encodes the immunogenic capsid protein. There are three genotypes of PCV2 (PCV2a, PCV2b, and PCV2c), but vaccines available for PCV2 infection are only targeted against PCV2a. The objective of this thesis was to create viable chimeric PCV2 viruses with an ORF2 displaying …
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Molecular Pathogenesis and Development of a Genetically Engineered Vaccine for Type-2 Porcine Circovirus
… of this dissertation were to biologically, genetically and experimentally characterize both PCV1 and PCV2, to identify the genetic determinant(s) for virulence and replication, and to develop an effective genetically-engineered vaccine against PCV2 infection and PMWS. The genetic …
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Genetic characterisation and phylogenetic analysis of bovine astroviruses and kobuviruses
… from diarrhoeal disease. Complex etiopathogenesis involving the infection of one or multiple pathogens, as well as other non-infectious factors such as the environment and nutrition, contributes to its devastating effects. Astroviruses (AstVs) and Kobuviruses (KoVs) are two single-stranded, …
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Development of a potential challenge model and plant-produced vaccine candidate for beak and feather disease virus
… genome sequences acquired to infer phylogenetic relatedness. The capsid gene (cp) was optimised for transient Agrobacterium-mediated expression in whole-plant Nicotiana benthamiana (N. benthamiana). Virus-like particles (VLPs) were purified and analysed using transmission electron …
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Molecular and Serological Epidemiology of Swine Hepatitis E Virus from Pigs in Two Countries
… were amplified and sequenced for the ORF2 capsid gene region. Sequence analyses revealed that all the swine HEV isolates identified from pigs in Thailand and Mexico belong to genotype 3. Overall, the Mexican swine HEV isolates shared 89-100% sequence identity to each other, and about 89-92% …
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Cross-Species Infection and Characterization of Avian Hepatitis E Virus
… mobility assays (HMA) were developed to genetically differentiate field strains of swine HEV and avian HEV from known reference strains. It was shown that the HMA profiles generally correlate well with nucleotide sequence identities and with phylogenetic clustering between field strains …
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Vesicular Stomatitis Virus as a Vector to Deliver Virus-Like Particles of Human Norovirus: A New Live Vectored Vaccine for Human Norovirus
… be ideal for controlling this disease. The major capsid gene (VP1) of a human NoV was inserted into the VSV genome at the glycoprotein (G) and large (L) polymerase gene junction. Recombinant VSV expressing VP1 protein (rVSV-VP1) was recovered from an infectious cDNA clone of VSV. Expression of the …
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Documenting the Patterns and Ecological Impacts of a Densovirus on Melissa Blue Butterflies (Lycaeides melissa) and Beyond
… disease in beneficial species, or expanding on general knowledge of disease dynamics. Insect-specific viruses from the family Baculoviridae are known for their host specificity and lethal effects, however, viruses (like their insect hosts) are enormously diverse and many avenues of their …
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Molecular Characterization of Animal Strains of Hepatitis E Virus (HEV): Avian HEV and Swine HEV
… Studies showed that swine HEV and avian HEV are genetically and antigenically related to human HEV, and that pigs and chickens are useful animal models to study HEV replication, pathogenesis and cross-species infection. The objectives of this dissertation were to genetically characterize both …