Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 35 for “"capacitation"”.
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Acrosomal molecules exposure during human sperm capacitation
… acrosome, but is accessible after sperm undergo capacitation.When the sperm population isundergoing capacitation, the number of cells displaying zonadhesin is significantly increased potentially reflecting their fertilizing capacity. The objective of this study is to evaluate this event on human …
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Choline Acetyltransferase and Carnitine Acetyltransferase Activity in Human Spermatozoa During Capacitation
… in human spermatozoa is altered during in vitro capacitation and it correlates with the fertilizing potential of sperm.</p> <p>Regional immunoreactivity in human spermatozoa as assessed by fluorescent immunocytochemistry was compared with ChAT and CaAT activity determined by enzymatic …
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Female Reproductive Tract Fluids and Their Influence on Capacitation of Bovine Spermatozoa
Uterine, oviducal and follicular fluids were analyzed for protein, steroids and metabolic substrates based on stage of the cycle and follicular size respectively. Uterine fluids contained more protein than oviducal fluids and pyruvate and lactate concentrations in both fluids showed an inverse …
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Regulation of SNARE function during sperm capacitation to promote the acrosome reaction
… of how the acrosome reaction is regulated during capacitation so that the molecular defects that cause infertility can be identified. I hypothesized that capacitation alters sperm protein phosphorylation promoting soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) …
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The xenogenous capacitation response of fresh, cooled/extended and frozen/thawed equine semen as determined by a chlortetracycline stain
… to investigate the possibility of xenogenous capacitation, using stallion spermatozoa. Ewes were grouped according to endocrine status as anestrus (n = 12) or estrus (n = 11) and were surgically inseminated with either fresh (FR), cooled/extended (FCE) or frozen/thawed (FZ) spermatozoal …
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Identification of platelet activating factor (PAF) receptor in equine spermatozoa and its role in motility, capacitation and the acrosome reaction
… synthetic PAF on stallion spermatozoal motility, capacitation, and the acrosome reaction (AR) were evaluated. Treatment of 10 stallion semen samples with 10 â 4 to 10 â 13 M PAF resulted in statistically significant differences in motility and capacitation (r2 = 0.81 and 0.83 respectively). The …
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Re-localization of SNARE proteins in mouse sperm prior to the acrosome reaction
… in mouse epididymal sperm before and after capacitation. Sperm were fixed and incubated with antibodies to syntaxin, synaptobrevin and then fluorescent secondary antibodies. Super resolution Structured Illumination Microscopy (SR-SIM) was used to examine samples collected at 0, 10, 30, 60, …
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Sperm release from the oviduct reservoir
Sperm undergo capacitation while traveling through the female reproductive tract, a process including several biochemical and physiological events before fertilization. Also prior to fertilization, sperm bind to the epithelial cells of the oviduct isthmus to form a sperm reservoir. This sperm …
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Economic development and women empowerment in Zamimpilo art and craft co-operative
… the extent to which participation, empowerment, capacitation and benefits of membership contribute in enabling cooperatives to empower women. This study applied the Capability Approach (CA) in an attempt to understand the need and importance of capabilities to women in a cooperative. The quality …
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Regulation of boar sperm function by the oviduct – formation of a sperm reservoir, modulation of Ca 2+ influx, and release from storage
… and is implicated in the regulation of capacitation. Once capacitated, a small number of sperm is released from the storage site to form a pool that can bind to the egg. The precise identity of the molecules that mediate the interactions between spermatozoa and epithelial cells of the …
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Characteristics of sperm binding to the mouse oviduct
… keeps sperm in a quiescent stage due to delayed capacitation and suppressed motility. The objective of this study was to determine different characteristics of sperm binding to the oviduct. The study was conducted using the translucent mouse oviduct ex vivo. The specificity of sperm binding was …
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Zinc ion fluxes on the pathway to mammalian sperm fertilization competency
… mammalian spermatozoa for fertilization, sperm capacitation, was first described in 1951, yet its regulatory mechanisms remain poorly understood. Here, we document a novel biological phenomenon of a unique Zn2+ distribution (further zinc signature) associated with mammalian spermatozoa from the …
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Characterization of the lipid composition of washed and percoll gradient centrifuged epididymal mouse sperm.
Capacitation is an important yet poorly understood process during which the fluidity of the sperm membrane increases to prepare sperm for the acrosome reaction (AR) and subsequent sperm-egg binding. While cholesterol efflux may be partially responsible for this increase, modification of lipid …
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Structural and functional aspects of sperm after transit through the fallopian tube of the sheep
… maturational changes include the processes of capacitation and the acrosome reaction. The Fallopian tube has been accepted as the site of completion of these events while their initiation may occur either in the uterus or in the Fallopian tube. The role and/or interaction of the Fallopian tube …
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Investigation of galactosyltransferase and α-lactalbumin-like proteins in mammalian reproductive tracts / by Yulu Tang
… of the female reproductive tract, where sperm capacitation and fertilization occur; and, whether GalTase is also the sperm's receptor for zona in other species than mouse.
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The response of human spermatozoa to chemoattractants
… in [Ca\(^{2+}\)]i, The response was dependent on capacitation. In low-Ca\(^{2+}\) or EGTA-buffered saline the response to bourgeonal was inhibited. Pretreating spermatozoa with bis-phenol (20μM) to release stored Ca\(^{2+}\) did not alter the response. Thus bourgeonal acts primarily by inducing …
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Regulation of amnion induction during the human formative pluripotency transition
… stage in acquiring multi-lineage competence. Capacitation, initiated by MEK/ERK inhibition withdrawal, drives naïve hPSC into a formative like phase in vitro. Amnion is an extraembryonic membrane that supports the embryo by providing protection, nutrient exchange, and signalling cues. Unlike …
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The combinative effect of soluble suLeA glycan and specialized bovine semen extender, FSRD4+, on bull sperm quality
… maintained motility. Additionally, sperm undergo capacitation to reach final maturation. Capacitation will allow the sperm to hyperactivate and penetrate the cumulus cells and the zona pellucida, and fertilize the oocyte. The sperm-oviduct binding and subsequent sperm reservoir formation is …
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Assessing Stakeholder Perceptions in Participatory Infrastructure Upgrades - a Case Study of Project Silvertown in Cape Town
… and decision making • there was poor capacitation of community members in the participatory process • there was illegitimate representation of the residents by community leaders Findings of the conflicts between stakeholders in a given system can contribute towards identifying what …
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