Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 10 of 10 for “"cap independent translation"”.
-
eIF4E Phosphorylation Balances Cap-dependent and Cap-independent Translation Initiation
<p>Signaling pathways converge on the translation machinery and influence protein synthesis globally or specifically on certain classes of transcripts. The experiments described in this thesis focus on regulation of translation initiation through the cap-binding protein eIF4E. </p><p>Aberrant …
-
The Role of Secondary and Tertiary Structure in the Cap-Independent Translation of FGF-9 and HIF-1-alpha
<p>Under normoxic conditions, eukaryotes initiate translation of RNA through eIF4E recognition of the 5’ cap. However, under cellular stress, eukaryotic translation must be initiated through a 4E-independent, or “cap-independent” mechanism, involving eukaryotic initiation factor 4G (eIF4G) binding …
-
Direct Recruitment of eIF4GI and/DAP5 to the 5' UTR of a Subset of Human mRNA Drives Their Cap-independent Translation
… nutrient deprivation, etc.), the canonical, cap-dependent translation initiation pathway in human cells is suppressed by sequestration of the cap-binding protein, eukaryotic initiation factor(eIF) 4E, by 4E-binding proteins. Circumvention of cap-dependent translation shutdown has been linked …
-
The Interaction Between Eukaryotic Translation Initiation Factor eIF4G and 3’ Cap Independent Translation Element of Barley Yellow Dwarf Virus Is Affected by Multiple Initiation Factors
… Virus (BYDV) lacks a 5’ (7-methyl guanosine) cap as well as a 3’poly A tail. Like many plant viruses, BYDV contains a cap independent translation element (CITE) in the 3’ untranslated region of the viral mRNA. BTE (Barley Yellow Dwarf Virus like cap-independent translation element) is one of …
-
Eukaryotic Initiation Factor 3 Interactions with Structural Elements of Barley Yellow Dwarf Virus Untranslated Regions Reveal Details of a New Cap-Independent Translation Initiation Model
… translates without using a 5′ 7-methylguanosine cap or a 3′ poly-adenosine tail, features that are required for canonical mRNA translation. BYDV’s non-canonical translation relies on RNA structures in the 5′ and 3′ untranslated regions (UTRs) to recruit eukaryotic initiation factors (eIFs) and …
-
Human La Function in Expression of Coding Transcripts
… La proteins are associated with promoting cap-independent translation from the internal ribosome entry sites (IRESs) of several cellular and viral coding RNAs. In this study, we investigated the molecular mechanisms by which La interacts with coding transcripts. We show that in addition to …
-
Translation of Partially Decayed Messenger RNAs in Yeast
… cricket paralysis virus (CrPV IRES) to allow for cap-independent translation of the decay-protected gene, <em>LacZ</em>, encoding the enzyme β-galactosidase. Through the use of primer extension, βgalactosidase assay, and western blots, the results indicate that the partially-decayed RNAs are …
-
Structural Basis For Poliovirus Cloverleaf-Mediated Regulation Of Genome Replication
… which are characterized by small, non-enveloped capsids containing short, positive-sense single-stranded RNA genomes. During the viral replication cycle, it is critical that the virus generates the appropriate amounts of viral proteins and genomic RNA. Upon entry in the cell, the viral genome is …
-
Point mutational examination of eukaryotic initiation factor 4E (eIF4E), inclusion bodies analysis, and Barley Yellow Dwarf Virus (BYDV) exonuclease resistant RNA structure mapping and prediction
… initiation factor 4E (eIF4E) binds the 5’m7GTP cap structure of mRNA in order to facilitate effective translation. Recessive eIF4E alleles harboring naturally occurring point mutations have been associated with resistance to viral infection, particularly in members of the genus Potyvirus. …
-
Investigations into the role of RNA modification in the function of the c-Myc IRES
… This is mediated, in part, by a decrease in cap-dependent initiation. In several cases, it has been shown that cap independent pathways are used for the translation of mRNAs required as part of the cell stress response/permit cell survival, and internal ribosome entry segments (IRES) have …