Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 27 for “"calreticulin"”.
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New molecular and cellular aspects of mutant calreticulin in Myeloproliferative Neoplasms
Calreticulin (CALR) is an endoplasmic reticulum (ER) protein that plays an important role as a calcium (Ca2+) buffering chaperone. Mutations in CALR exon 9 have been identified in essential thrombocythemia and primary myelofibrosis, two myeloproliferative neoplasms (MPNs) characterised by …
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… functions and neuronal loss were affected by calreticulin and β-galactosidase, two proteins associated with ageing, chronic inflammation, and neurodegeneration. The aggregation of amyloid-β to form oligomers and insoluble amyloid plaques in the brain is a hallmark of AD. Amyloid-β can be …
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Detection of the calreticulin type 1 and type 2 mutations in a group of myeloproliferative neoplasm patients using molecular methods
… at exon 9 of CALR, the gene that encodes for calreticulin, were discovered through whole-exome sequencing and targeted re-sequencing in patients with MPNs. Among these mutations, more than 80% of CALR mutated patients possessed one of only two mutation types: type 1 (a 52-bp deletion) and type …
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Microglial activation and regulation by secreted chaperones
… (CNS) development, homeostasis and pathology. Calreticulin and LRPAP-1 are ubiquitously expressed protein chaperones that aid in protein folding and processing within the endoplasmic reticulum (ER). Both proteins might also be released into the extracellular space, but, if so, it is unclear …
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Microglial Phagocytosis of Bacteria and Synapses
… are not clear. In this work, involvement of calreticulin, galectin-3, apolipoprotein E and β-amyloid in microglial phagocytosis of bacteria were studied. Microglia activated with bacterial endotoxin upregulated and released extracellular calreticulin and galectin-3 in culture, and both …
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Biochemische und zellbiologische Charakterisierung des COP Vesikel vermittelten Proteintransports in Pflanzen
… COPI Vesikel wurde das ER residente Protein Calreticulin identifiziert. Dieses ließ auf eine Beteiligung der COPI Vesikel am retrograden Proteintransport vom Golgi-Apparat zum ER schließen und unterstützt die Hypothese, daß die Retention löslicher Proteine im ER nicht durch die Verhinderung …
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Elucidating the role of Mitoferrin (Mfrn), Iron Regulatory Proteins (IRP1 and IRP2) and Hephaestin (Heph) in Iron Metabolism by tagSNP and Protein-Protein Interaction (PPI) analysis
… analysis identified the excised band as Calreticulin (CALR). This is the first report of calreticulin having ferroxidase activity.
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Induktion von myokardialem ER-Stress durch biomechanische Last und neurohumorale Stimulation.
… 78kDa-glucose-regulated-protein (Grp78) und Calreticulin (CRT) in den rechten (RV) und linken (LV) Ventrikeln von Monocrotaline-behandelten Ratten und rechtsventrikulären Muskelstreifen, die verschiedenen Lastbedingungen und pharmakologischen Interventionen ausgesetzt wurden. Expression wurde …
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Analisi Molecolare del Melanoma
… 526 cells, 60s acidic ribosomal protein p1 and calreticulin precursor were more highly expressed than in melanocytes. These molecular differences may help in better understanding melanoma development and its different responsiveness to therapies. The identified proteins could be exploited as …
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Acute Nitrate Exposure Causes Proteomic Changes Consistent with the Regulation of Reactive Oxygen and Nitrogen Species
… homeostasis (heat shock protein 70, prohibitin, calreticulin, and proteasome &#;-type 4 subunit), glycolysis (enolase), transport of hydrophobic molecules (lipocalin) and cytoskeletal arrangements (intermediate filaments and a gelsolin-like adseverin). The most parsimonious explanation for these …
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Serpin polymers enforce molecular filtration in the endoplasmic reticulum
… ER stress, and overexpression of ER chaperones calreticulin and BiP promotes this solidification and increases the stiffness of the resultant protein matrix. Single particle tracking of ER proteins revealed that this process initiates in cells with normal reticular ER morphology. This novel …
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Coordination of the endoplasmic reticulum stress response and lipid metabolism in plants
… (BiP), protein disulfide isomerase (PDI) and calreticulin. Certain aspects of the ER stress response may be unique to plants. A seed-specific result of the ER stress response was the accumulation of triacylglycerols (TG), which specifically increased in the endosperm of the fl2 mutant to more …
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Investigating the ablative and immunomodulatory effects of high frequency irreversible electroporation on osteosarcoma in-vitro
… approach, 3) Evaluating DAMPs (ATP, calreticulin, and HMGB1) post-H-FIRE ablation of human OS cells. Overall, our study showed that H-FIRE successfully ablated OS cells in-vitro, induced the release of DAMPs from treated cells, and promoted activation signatures in immune cells. This …
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Mass Spectrometric Characterization of the MCF7 Cancer Cell Line: Proteome Profile and Cancer Biomarkers
… 14-3-3-sigma, antigen Ki-67, TP53RK, and calreticulin were identified. These data were generated by subjecting to mass spectrometric analysis ~42 µg of protein digest, analyzing 16 SCX peptide fractions, and interpreting ~55,000 MS2 spectra. Total MS time required for analysis was 40 h. …
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Investigating the molecular biology of aging and extended longevity: Proteomic and genomic analysis of mouse liver
… protein disulfide isomerase (PDI), and calreticulin as exhibiting a specific age-associated increase in carbonyl content. This increase in oxidative damage to critical ER proteins in aged liver strongly indicates an impairment in protein folding, disulfide cross-linking, and …
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Preclinical Evaluation of Immunomodulatory Effects of Aurora Kinase Inhibition In Human Papillomavirus Positive Cancers
… significantly higher cell surface expression of calreticulin (CRT), and high mobility group box 1 protein (HMGB1) in the culture supernatants, both of which are markers for (ICD). Studies with the mEER tumor cells implanted in immunocompetent syngeneic mice showed partial in vivo efficacy of …
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Content and Activity of Cytochrome P450 3A in Rat Brain Microsomes and Mitochondria
… by Western blot analysis of the microsomal (calreticulin) and mitochondrial (VDAC) markers. Different concentrations of midazolam were incubated with rat brain microsomes and mitochondria in a side-by-side comparison over a period of 10 minutes.</p> <p>The method was developed and optimized …
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Proteomic Analysis of Human Mesenchymal Stem Cell Aging during serial sub-culture
… and database searching. It was observed that Calreticulin, Glyceraldehyde-3-phosphate dehydrogenase, and alpha-crystallin B chain were up regulated in protein expression while Moesin (C-terminal frag), ii Poly (rc)-binding protein, Alpha-tropomyosin, Annexin A2, and Galectin 3 were down …
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Distinct functional effects of NMDA receptors in normal and leukaemic megakaryocytes
… only in 2013 through the discovery of Calreticulin mutations in approximately 30% of patients with megakaryocytic neoplasias. The role of Ca2+ and glutamate pathways in megakaryocytic malignancies remains unknown but their oncogenic potential has already been demonstrated in other …
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Anbindung und Aufnahme von Hitzeschockprotein-Antigenkomplexen durch Antigenpräsentierende Zellen
… die Hitzeschockproteine Hsp90, GRP94, Hsp70 und Calreticulin auf Raw264.7 Zellen beschrieben. Unter den in der vorliegenden Arbeit verwendeten Bedingungen exprimierten Raw264.7 Zellen sowohl vor als auch nach LPS Stimulation funktionelles CD91, wobei die Fähigkeit Hsp70 zu binden nach Stimulation …
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