Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"calprotectin"”.
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Bioinorganic Chemistry of the Human Host-Defense Protein Calprotectin
… by releasing the metal-chelating protein calprotectin (CP) at sites of infection and in the upper layers of the epidermis. CP is a Mn(II)- and Zn(ll)-binding protein. The work described in this thesis elucidates the metal-binding properties of CP, and correlates these properties with in …
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Metal sequestration of the host-defense protein human calprotectin
… factors is the abundant neutrophil protein calprotectin (CP, Si 00A8/S 1 00A9, MRP-8/MRP- 14 oligomer, calgranulins A and B). This calcium (Ca)-binding EF-hand domain protein exhibits two transition-metal-binding sites at the Si 00A8/S 1 00A9 heterodimer interface, and Ca(II) coordination …
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Investigation of microbial responses to transition metal sequestration by the innate immune protein calprotectin
… thereby inhibit microbial growth and virulence. Calprotectin (CP) is an abundant antimicrobial protein that is released from neutrophils and epithelial cells at sites of infection. CP sequesters divalent first-row transition metal ions and thereby limits the availability of essential metal …
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Measurement of calprotectin (S100A8/S100A9) and S100A12 in serum: method development, analytical validation, and clinical application
… of intestinal inflammation, in particular faecal calprotectin and to a lesser extent faecal S100A12, are used to discriminate between inflammatory bowel disease (IBD) and irritable bowel syndrome (IBS) and categorise active from inactive disease in established IBD. Faecal biomarkers have …
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A biophysical and biochemical approach to understanding the interplay between Quaternary structure and function of human calprotectin
… of essential metal nutrients. Humans release calprotectin (CP), a heterooligomer of S100A8 and S100A9, from neutrophils and epithelial cells to prevent microbes from accessing manganese, iron, nickel, and zinc. CP also binds Ca(II) ions, which increases the transition-metal affinity and …
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Exploring the spectrum: The heterogeneity of autism and associations with gastrointestinal inflammation
… which found no group differences in studies of calprotectin and lactoferrin. This synthesis was however limited by methodological concerns and highlighted the need for large community-based studies of GI inflammation including non-autistic siblings and representative controls. This gap is …
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Evaluating the contribution of a metal independent aldolase to resisting nutritional immunity
… this defense is the metal binding host protein calprotectin, which inactivates metal- dependent bacterial enzymes. The ability of Staphylococcus aureus to cause infection suggests that it has mechanisms that enable it to minimize the impact of nutrient metal limitation. The consumption of …
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¿CHIOS MASTIC GUM FROM PISTACIA LENTISCUS AS A MODULATOR OF INFLAMMATION AND INTESTINAL BARRIER FUNCTION: AN IN VITRO AND CLINICAL INVESTIGATION.¿
… questionnaires and faecal biomarkers (calprotectin, zonulin), were utilized to assess clinical outcomes. Concurrently, in vitro experiments compared Selpic with its isolated Supermastic® triterpene fraction in Caco-2 cells and lipopolysaccharide-stimulated THP-1 macrophages. Clinically, …
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Effectiveness of visceral osteopathic treatment on gastrointestinal indicators and behaviour patterns in autistic children: using questionnaire and biochemical markers to measure outcomes
… were analysed for three biochemical markers, calprotectin, M2-pyruvate kinase and lactoferrin. Forty nine children, between 3½ and 8 years old, and independently diagnosed as autistic by specialist professionals were recruited. Questionnaires using a 10 point Likert scale assessed behavioural …
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Microbial, immunological, phenotypic and genetic markers of risk: aspects of Crohn’s disease that are shared by unaffected siblings
… intestinal permeability and raised faecal calprotectin, are also abnormal in some relatives of patients with CD. By using the combination of genotype and biomarkers models of disease prediction become a realistic possibility, and may permit intervention to prevent disease onset. …
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Mapping the microbiota in hidradenitis suppurativa
… on total DNA derived from the samples. Calprotectin was determined by enzyme-linked immunoassay (CALPROLAB™ Calprotectin ELISA (HRP). Complement C5a levels were assessed using enzyme-linked immunosorbent assay (Abcam ab193695 Complement C5a Human ELISA Kit). Pain was widely reported in …
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Nutritional aspects and gut microbiota in paediatric inflammatory bowel disease
… serial stool samples and correlated with faecal calprotectin levels, systemic inflammatory markers and clinical activity. A single stool sample was collected from their first-degree relatives and two serial samples from healthy children with no family history of IBD. Significant changes were …
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Systemic sclerosis. Novel molecular and epidemiological features of disease.
… protein complex S100A8/A9, also known as calprotectin, has been associated with several rheumatic diseases. Faecal calprotectin (FC) is a validated biomarker in inflammatory bowel disease. We have explored the biomarker potential of FC in SSc. FC correlated with SSc manifestations in the …
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Changes in the Oral Cavity and Common Diseases /// Промени в оралната среда и общи заболявания
… 30% of dental technicians. Tracking the level of calprotectin in saliva reveals a decrease in values after antibiotic treatment and outlines a new marker for monitoring patients with periodontal problems. Half of Bulgarian patients have dental amalgam. The greatest amount of mercury is released …
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Analysis of fecal biomarkers to impact clinical care and public health
… samples, and can also be used to quantify fecal calprotectin. Finally, I present the first ever analysis of the microbiome and metabolome of wastewater collected from a residential neighborhood. By testing samples collected hourly over one day, we identified thousands of bacteria and metabolites …
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A molecular investigation of the antimicrobial functions of the human S100 host-defense proteins
… proteins of the S100 family of proteins: calprotectin (CP), S100A12, S100A7, and S100A15. In the first half of this thesis, we elucidate the Zn(lI)-binding and antimicrobial properties of S100A12 and S100A7 through the use of solution and microbiology studies. We evaluate the affinity of …
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METAL TRAFFICKING DURING LIMITATION AND EXCESS IN BACILLUS SUBTILIS
… human neutrophils and epithelial cells secrete calprotectin, an abundant antimicrobial protein, which has been shown to sequester zinc and iron from bacterial pathogens such as Staphylococcus aureus, preventing infection. In Bacillus subtilis, a model system for low G+C Gram-positive bacteria, …
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The relationship between enteral nutrition, energy metabolism and gut homeostasis during the course of critical illness.
… was required for the assessment of faecal calprotectin, gut microbiota and their metabolites while serum samples were used for the analysis of inflammatory cytokines, and intestinal injury biomarkers. Overall this project has recorded a cross-link between feed, gut homeostasis with systemic …
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Colonic metabolism of dietary grape seed extract: Analytical method development, effect on tight-junction proteins, tissue accumulation, and pan-colonic pharmacokinetics
… protein occludin and reduced levels of fecal calprotectin, which suggests an improvement of gut barrier integrity and a potential modulation of endotoxemia. Additionally, chronic supplementation of the diet with flavanols did not increase colonic tissue accumulation of PCs or their microbial …
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Local effects of Linoorbitides and enterolactone On intestinal epithelial functions
… less variation than other biomarkers like fecal calprotectin and fecal lactoferrin. Lastly, altered cholesterol trafficking was observed in Caco-2 cells, an effect best attributed to the glucuronic acid conjugated form of enterolactone. These studies confirmed the importance of the flaxseed …
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