Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 19 of 19 for “"callose"”.
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ULTRASTRUCTURE, IMMUNOCYTOCHEMISTRY, AND BIOINFORMATICS OF SPORE DEVELOPMENT IN THE MOSS PHYSCOMITRELLA AND THE HORNWORT DENDROCEROS
… and immuno-electron microscopy localizes callose in the aperture of developing spores in the model moss <italic>Physcomitrella</italic>. Twelve copies of callose synthase genes were annotated bioinformatically and compared with Arabidopsis callose synthase genes. This study identifies a …
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Biochemical and histological investigations of viral localisation in the hypersensitive reaction of Phaselous vulgaris L. var Pinto to tobacco mosaic virus infection
… inoculation. Histochemical tests indicated that callose formation was initiated at this time, and occurred in response to necrotisation. Inhibition of necrosis by either ascorbate infiltration or elevated temp8rature treatment resulted in the absence of callose deposition. Scanning …
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Towards understanding of plasmodesmal association and regulation using Plasmodesmata-Located Protein 5 as a model
… or npr1, using PD permeability assays and PD callose quantifications showed that, SA accumulation is critical for PDLP5-mediated PD-callose deposition and -closure during microbial pathogen infection.
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Genes and mechanisms in Arabidopsis innate immunity against Leptosphaeria maculans
… found to be responsible for L. maculans induced callose depositions. A segregant analysis of the transcriptomes from resistant and susceptible Col-0 x An-1 F3 lines revealed a region on chromosome 4 with genes significantly more highly expressed in the resistant progenies. T-DNA insertion lines …
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A multiscale study of plasmodesmata: from channel ultrastructure to plant architecture
… phloem unloading in lines that over-accumulate callose, a polysaccharide occluding plasmodesmata. The protein might specifically control correct folding of callose synthase enzymes within the endoplasmic reticulum. Lastly, looking at the whole plant level, I show that induced closure of …
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Characterisation of phytoalexin accumulation in maize inoculated with Cercospora zeina, the causal organism of grey leaf spot disease
… molecular pattern (PAMP), and a subsequent callose assay showed positive induction of a callose defence response. However, gene expression and phytoalexin accumulation did not change following chitosan treatment, although zealexin accumulation was higher than kauralexins. Previous studies …
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The role of abscisic acid in the defence response of tomato (Solanum lycopersicum) to the necrotrophic pathogens Botrytis cinerea and Erwinia chrysanthemi
… border of the infiltration zone. Deposition of callose at the site of pathogen entry was previously demonstrated to be influenced by ABA. We have shown that callose deposition after B. cinerea inoculation is weaker in sitiens compared to the wild type. Inhibition of callose synthesis with …
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Investigation of the molecular basis of PAMP-induced resistance
… namely camalexin, glucosinolates and callose, in PAMP-triggered immunity (PTI) against the phytopathogenic bacterium Pseudomonas syringae pv. tomato (Pto) DC3000. These are well known active defences Arabidopsis employs to restrict fungi and oomycetes invasion. Results showed that …
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THE SPECIAL WALLS AROUND GAMETES IN CERATOPTERIS RICHARDII AND AULACOMNIUM PALUSTRE: USING IMMUNOCYTOCHEMISTRY TO EXPOSE STRUCTURE, FUNCTION, AND DEVELOPMENT
… and Aulacomnium. The preponderance of callose and hemicelluloses in the walls of the male gametes of Ceratopteris and Aulacomnium, respectively, and the importance of these polysaccharides in development are discussed. Taken together, the studies that comprise this dissertation advance …
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Analyses of a genic male-sterile mutant in soybean
… that sterility was caused by retention of the callose walls, which normally are degraded by callase ([beta]-1,3-glucanase) at the late tetrad stage. Molecular markers linked with the male-sterile gene were identified by using an F2 population of 111 individuals. Analysis revealed that the …
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Glucan synthase of Phytophthora sojae
… for glucan synthases from fungi and plants (callose synthases). Its apparent Km value for UDP-glucose was higher than reported for other glucan synthases. The activity was GTP-independent and shown not to be activated by divalent cations like Mg2+ or Ca2+, and shown to be inhibited by some …
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The effect of spirea aphid (Homoptera: aphididae) feeding and nitrogen fertilization on the growth of young apple trees, with comparisons to apple aphid
… at each nitrogen rate applied. Accumulation of callose at the phloem sieve plates in response to spirea aphid feeding occurred but to a lesser degree than from other aphids reported on apple and pecan leaves. Accumulation of fresh and dry weights in all tree parts (leaves, lateral shoots, trunk, …
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COMPARATIVE ULTRASTRUCTURE OF APICAL CELLS AND DERIVATIVES IN BRYOPHYTES, WITH SPECIAL REFERENCE TO PLASMODESMATA
… to LM5 and LM19 for HG unesterified pectins. Callose predominantly localizes at the PD neck region. This study provides the first documentation of changes in size and shape of AC with age in Physcomitrella patens gametophores. The PD densities of gametophytes examined in this study fall into …
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Identification and molecular characterisation of elicitor-priming for resistance in tomato against <i>Botrytis cinerea</i>
… decreasing necrotic lesion sizes and priming for callose deposition in a concentration-dependent manner. Furthermore, large-scale double (host/pathogen) transcriptomic analysis has unveiled that chitosan was able to prime 1,745 tomato transcripts during early and asymptomatic stages of <i>B. …
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Regulation of reserve carbohydrates in hull-less barley grain
… in the mutant grain. Deposition of arabinoxylan, callose and cellulose was altered in the absence of (1,3;1,4)-β-glucan in the mutant endosperm. QPCR analysis identified changes in the expression of starch and fructan biosynthetic genes during the storage phase. At grain maturity, sucrose and …
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Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment
… within root cortical cells. No evidence of callose deposition, a typical plant defence response, by the lupin root cells was observed after infected roots stained with aniline blue. Development of the model lupin-P. parasitica infection assay system facilitated ensuing studies of this …
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Phytophthora parasitica and lupin (Lupinus angustifolius) interactions: changes in gene expression during infection and after phosphite treatment
… within root cortical cells. No evidence of callose deposition, a typical plant defence response, by the lupin root cells was observed after infected roots stained with aniline blue. Development of the model lupin-P. parasitica infection assay system facilitated ensuing studies of this …
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Using the Bacterial Plant Pathogen Pseudomonas syringae pv. tomato as a Model to Study the Evolution and Mechanisms of Host Range and Virulence
Most plant pathogens are specialists where only few plant species are susceptible, while all other plants are resistant. Unraveling the mechanisms behind this can thus provide valuable information for breeding or engineering crops with durable disease resistance. A group of Pseudomonas syringae …