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Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"calli"”.
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Host range of Septoria glycines, quantification of partial resistance, and brown spot evaluation of soybeans regenerated from calli
… yield evaluation of soybeans regenerated from calli resistant to a pathotoxin produced by S. glycines were studied.
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Lipid Metabolism in Corn Tissue Culture and Molecular Biology of Soybean Seed Maturation
… tissue to study lipid metabolism. Embryogenic calli were also used to select cell lines resistant to cerulenin, an inhibitor of fatty acid synthesis. While it was hypothesized that the cerulenin-resistant calli might be an overproducing-oil line, the selected resistant calli did not exhibit an …
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Application of in vitro methods to improve soybean resistance to Fusarium solani, the causal agent of sudden death syndrome
… caused by the soilborne fungus, Fusarium solani. Calli reactions of five soybean cultivars grown on culture medium amended with fungal culture filtrate were compared to the reactions of the same five cultivars inoculated with the fungus under greenhouse conditions. A significant positive …
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Inheritance of competencies for leaf disc regeneration, anther culture, and protoplast culture in Solanum phureja and correlations among them
… isolation. Cells of NBP2 divided to form calli and shoots in protoplast culture, but the clone did not respond to anther culture or leaf disc regeneration. All the individual plants in the F₁ generation were responsive to both anther culture and protoplast culture; however, there was …
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Genetic Improvement of Switchgrass Cell Wall Content, Leaf Angle and Flowering Time
… bioenergy crop. Somatic embryogenic (SE) calli are used for genetic transformation in switchgrass. A superior switchgrass line, HR8, was developed using recurrent tissue culture selection from cv. Alamo. HR8 SE calli were genetically transformable using Agrobacterium at an efficiency of …
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The study of wheat striate mosaic virus (WSMV) in wheat callus tissue culture
… of thin sections derived from virus-infected calli showed WSMV particles both within the nuclear membranes and in the cytoplasm. Except for the presence of virus, there did not appear to be any difference in the ultrastructure of WSMV-infected and healthy calli. Callus tissue consisted of …
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Transformation of Digitaria sanguinalis with XvPer1, a novel antioxidant
… from immature inflorescences. Embryogenic calli generated was transformed with XvPerl using the Biolistic gene gun. XvPer 1 shares high similarity to a recently discovered group of antioxidants, the 1-Cys PRX's. A homologue was shown to be absent in D. sanguinalis by northern, Southern and …
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De novo morphogenesis on tomato thin cell layers and variation for genetic recombination among plantlets regenerated from tissue culture
… on thin cell layer explants than on cotyledon calli, or micropropagated shoot tips. Genetic recombination rates and map distances were compared among hybrid plants grown in the greenhouse and regenerated from the aforementioned tissue culture systems. Increased recombination rates and map …
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Callus Development and Organogenesis in Cultured Explants of Cowpea (Vigna unguiculata (L.) Walp
… in the formation of callus and rhizogenesis. Calli that were formed were separated into six classes based on color and texture. Yellowish friable callus, yellowish compact, soft yellowish callus and green and white were composed of largely vacuolated cells and were non-regenerative. Friable …
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Maize Transformation for Resistance to Aspergillus Flavus
… were delivered into H99 and Pa91 type I calli by particle bombardment. Sixteen different transformation events were obtained from H99 calli. Some were crossed with Pa91 the untransformed control and then self-pollinated up to six times. The transgenes have been confirmed to be integrated …
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Expanding the genetic variability of flatpea using tissue culture, mutagenesis, and intercrossing techniques
… for callus initiation; after two subcultures, calli were transferred to a regeneration medium containing 0.1 µM IAA and 1.0 µM zeatin. Calli and regenerated root tissue contained 47 and 381 as much DABA as leaf tissue from 'Lathco' flatpea plants, respectively. Initial tests established that …
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Propagation of asparagus (Asparagus officinalis L.) via somatic embryogenesis
… formation was determined. High frequency of calli initiation was observed in darkness. A soft, translucent and highly friable callus was also produced in darkness while the green and compact callus was observed in light condition. To investigate the importance of genotypic effects on callus …
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Contributions to the systematics of the genus Schizochilus
… to be no mention of the basal tubercles or calli on the lip.
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MOLECULAR NETWORK CONTROLLING THE OVULE DEVELOPMENT IN ARABIDOPSIS THALIANA
… Adventitious shoots regenerated from mutant calli form the double mutant flowers, which also display the reduction of number of ovule primordia (Ishida et al., 2000). The goal of this work was to investigate the interaction among ANT, CUC1, CUC2 genes to determine ovule primordia formation.
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Genome editing with CRISPR-Cas9 in the Illinois long term selection experiment
… editing vectors were delivered to embryogenic calli using biolistics and transgenic events selected. Multiple albino plants indicative of biallelic mutations were recovered in the ILP1 genotypes at 1.5% efficiency; however none were produced from the IHP1 genotype. A second CRISPR experiment …
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Somatic Embryogenesis of Pathogen-Free Horseradish (Armoracia Rusticana) Plants in Vitro
… Since cell suspensions of somatic embryogenic calli yielded plants, it raised the possibility to generate large number plantlets from suspension cultures. In addition, it was predicted that SEs could be encapsulated to make ""artificial seeds"" that could be used to supply a certified set …
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Somatic embryogenesis of pathogen- free horseradish (Armoracia Rusticana) plants in vitro
… Since cell suspensions of somatic embryogenic calli yielded plants, it raised the possibility to generate large number plantlets from suspension cultures. In addition, it was predicted that SEs could be encapsulated to make “artificial seeds” that could be used to supply a certified set program.
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Generation of Transgenic <i>Medicago Sativa</i> Overexpressing "<i>Osmotin-Chitinase</i>" Gene Chimera
… explants of <i>Medicago</i>. Transformed calli were grown on callus inducing medium containing kanamycin for screening. Further screening of the positive transgenics was performed using PCR. Southern hybridization was carried out for further confirmation of successful transformation. …
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Periostin-like-factor in tissue repair and remodeling
… PLF is highly up regulated in fractured bone calli, albeit not present in normal adult bone. Furthermore PLF leads to increased bone formation in vivo in the bone marrow cavity when over expressed adenovirally. These results suggest that PLF promotes tissue repair and healing after injury. …
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In Vitro Regeneration and Genetic Transformation of Sesbania Drummondii: A Medicinally and Environmentally Important Plant
… medium resulted in its mass proliferation of calli having numerous embryoid-like structures. Indole-3-butyric acid (0.24 - 2.46 |aM) was found suitable for root induction. In vitro regenerated plants were acclimatized in greenhouse conditions. This report is a first report on studies of in …
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