Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 46 for “"cadherins"”.
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Dissecting the Functions of Classical Cadherins in Skin
… is know about the contribution of classical cadherins to epidermal morphogenesis. Genetic dissection of cadherin function in skin is complicated by overlapping expression of E- and P-cadherin and the fact that their genes are tightly linked, precluding conventional conditional knockout …
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Regulation of Homophilic and Heterophilic Adhesion Among Classical Cadherins
Finally, fluorescence imaging assessed cadherin clustering and actin organization in E-cadherin expressing cells cultured on E-, N-, and C-cadherin ectodomains. As expected, the formation of filamentous actin correlated with the Rac activation amplitudes in E-cadherin expressing cells. The results …
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Tension Propagation Along Tip-Link Cadherins: Regulation and Implications for the Auditory System
<p>Hair bundles detect sound by shearing in response to vibrations spanning orders of magnitude in intensity and frequency. Their responsiveness stems from mechanosensitive ion channels that sit atop the stereocilia and are gated by tension in tip links. Experimental evidence and theoretical …
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Investigation of minimum fragment requirement for mimicking n-cadherin mediated adhesion
Cadherins are transmembrane glycoproteins that are involved in maintaining integrity of cell junctions, morphogenesis, cell sorting and many more important biological processes. In my thesis, I have focused on studying the binding affinity of a class of cadherin superfamily called N-cadherins. …
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Kinetics and regulation of the initial stages of cadherin-mediated adhesion
… and in the maintenance of tissue integrity. Cadherins are calcium-dependent adhesion proteins which mediate cell adhesion in soft tissues. Several theories have been offered to explain the mechanism of cadherin adhesion. The first proposed mechanism put forth held that cadherins mediated …
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Reexamining actin polymerization in maintaining cadherin mediated cell-cell adhesion
… contractile actomyosin networks which pull on cadherins and strengthen the mechanosensitive bonds (the contractility model) or the fence around clusters of cadherins which mediate stronger adhesion than single cadherins (the clustering model). Actin polymerization could no doubt achieve these …
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Cadherin specificity in adhesion and mechanotransduction
Cadherins mediate Ca2+ dependent cell-cell junction in all animal tissues. Tight regulation of cadherin adhesion plays critical roles in diverse biological processes. Differential binding between cadherin subtypes is widely believed to mediate cell sorting during embryogenesis. However, a …
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Investigation of Neural and Epithelial Cadherin Monomer Properties Using a Molecular Dynamics Approach
… and conformation are similar for the two cadherins, there are several physical properties that differ: dimerization affinity, dimerization kinetics, intermediate structure, and critical residues for dimerization. In the presence of calcium, cadherins are in equilibrium between monomeric …
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Structural Basis of Adhesion Activation of the Cadherin-Catenin Complex
… and cancer metastasis. Type I classical cadherins such as E-cadherin are composed of an extracellular domain of 5 extracellular cadherin repeats interspersed with calcium binding domains, followed by a single-pass alpha-helical transmembrane domain, then a C-terminal intrinsically …
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Examining the Role and Regulation of Cell-Cell Adhesion in Aggressive Prostate Cancer
… examine the efficacy of classical and desmosomal cadherins as prognostic biomarkers of aggressive prostate cancer. I began this study by characterizing the expression profile of desmosomal cadherins in normal human prostate and metastatic prostate cancer cell lines. This study revealed that DSG2, …
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Nickel Reduces Calcium Dependent Dimerization In Neural Cadherin
Cadherins are the primary transmembrane component in adherens junctions, structures that link the actin cytoskeleton in adjacent cells within solid tissues including neurological synapses, epithelium and endothelium. Cell-cell adhesion by cadherins requires the binding of calcium ions to specific …
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Developmental and Cellular Functions of Delta-Catenin
… with contributing functional links to cadherins and Rho family small G proteins.</p> <p>In examining delta-catenin’s nuclear roles, I identified delta-catenin as an interacting partner and substrate of the caspase-3 protease, which plays critical roles in apoptotic as well as …
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Regulation of cadherin adhesion at intercellular junctions
… of all multicellular organisms, and among them, cadherins are the major transmembrane cell-cell adhesion proteins in all vertebrate tissues. Regulation of cadherin mediated adhesion at cell-cell junctions is crucial to our understanding of development and disease. This thesis focuses on the …
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Biophysical Characterization Of A Proline Mutant Of Neural Cadherin
Cadherins are calcium dependent glycoproteins whose homophilic interactions mediate cell-cell adhesion in solid tissues. They are comprised of an extracellular region, a transmembrane region and a cytoplasmic region. The extracellular region plays a critical role in cadherin-mediated cell adhesion, …
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Homophilic and Heterophilic Interactions Among Cadherin Cell Adhesion Molecules
All three of the cadherins investigated in this thesis formed strong heterophilic bonds at three ectodomain alignments. Of the three, the inner bond (strongest) corresponding to full ectodomain overlap, did not exhibit much variation in either the homophilic or the heterophilic interactions. The …
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Characterizing the role of Nucleosome Remodeling Factor (NURF) in tumorigenesis and metastatic progression using mouse models of breast cancer.
… Our preliminary data shows dysregulation of E-cadherins, N-cadherins, and MHC-I genes in Bptf (an essential subunit of NURF) knocked down murine breast cancer cell lines. These proteins have well documented roles in the development and metastatic progression of cancers. To study the effect of …
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The Localization of β-catenin During Xenopus Kidney Development: A Look into β-catenin's Role in Kidney Development
… cell-cell adhesion: β-catenin interacts with cadherins to link cell junctions to the cytoskeleton. However, the dynamic localization of β-catenin is still poorly understood. This research will help gain a deeper understanding of junctional mechanisms that may regulate the Wnt pathways and the …
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PROBING CELLULAR MECHANO-SENSITIVITY USING BIOMEMBRANE-MIMICKING CELL SUBSTRATES OF ADJUSTABLE STIFFNESS
… properties of individual and clustered N-cadherins are examined in the polymer-tethered bilayer system in the absence of plated cells.
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Engineered N-cadherin substrates for stem cell differentiation
… To create this system, Fc-tagged N-cadherins are attached to alginate gels of varying stiffness through Protein A, which is chemically bound to the surface. Different cells known to express N-cadherin were seeded onto the gels, with the bone marrow stromal cells (BMSCs) showing the …
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Identification of exosomal proteins in primary human bronchial tracheal epithelial cell HBTE and the H358, THP1 and MCF7 cell lines
… were identified including integrins, catenins, cadherins, and cathepsins. Most of which are involved in molecular signalling, cellular growth and transport, supporting the role of exosomes in cellular communication. Several adhesion molecules such as integrins, laminlins, catenins as well as …
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