Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"cDNA microarrays"”.
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GeneSieve: A Probe Selection Strategy for cDNA Microarrays
… of thousands of genes simultaneously. Often, cDNA libraries representing expressed genes of an organism are available, along with expressed sequence tags (ESTs). ESTs are widely used as the probes for microarrays. Designing custom microarrays, rich in genes relevant to the experimental …
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Gene expression profiling of patients with Polycythaemia Rubra Vera using cDNA microarrays
… <br>pathogenesis of PV. This led us to perform cDNA microarray analysis of PV patients in order to get a more global view of gene expression in this myeloproliferative disorder. Granulocyte RNAs from 40 polycythaemia vera (PV) and 12 secondary erythrocytosis (SE) patients were analysed. …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… in processes of toxicity. We constructed microarrays using cDNA libraries derived from zebrafish embryonic and adult heart tissue. Embryonic heart arrays were used for protocol development. The resulting workflow was employed in the production of adult heart microarrays containing ~2800 …
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Identification and characterization of the activated defence response in the commercially important Agarophyte, Gracilaria Gracilis, following exposure to disease elicitors
… the first analysis of gene expression using cDNA microarrays in the red macroalga G. gracilis. Western hybridization analysis was used to establish whether the observed changes in gene expression following exposure to disease elicitors positively correlated to changes at the protein level.
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A Novel Subfamily of Three StAR-Related Lipid Transfer Proteins That Are Differentially-Regulated and Function in Intracellular Cholesterol Metabolism
… StarD5 and StarD6. StarD4 was identified using cDNA microarrays, as liver StarD4 expression decreased three-fold in mice fed a high cholesterol diet. StarD4 also sterol-regulated in cultured cells, and a functional sterol regulatory element (SRE) identified in its promoter. StarD4 was …
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Genetic Profiling in Soft Tissue Sarcoma
… histopathological subtypes were profiled using cDNA microarrays. Distinct gene expression patterns were identified in subtypes with specific translocations or mutations. Herein, frequent upregulation of developmental genes, from e.g. the Wingless and Hedgehog signaling pathways, was …
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Soybean Resistance and Defense Responses Against Aphis Glycines
… with and without soybean aphid infestation using cDNA microarrays consisting of over 18,000 soybean-expressed sequence tags. Eighty-four genes showed specific responses in Dowling and included genes related to defense and a number of other activities. The induction of the three defense-related …
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Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology
… of this dissertation were to 1) use custom-made cDNA microarrays to identify genes in the bovine pituitary gland that are differentially expressed during the estrous cycle and 2) characterize their patterns of gene expression. The estrous cycle is a dynamic process that requires coordination …
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The role of fibroblast growth factor-2 (FGF2) in vascular remodeling and adaptation
… in the ischemic limb as evaluated using cDNA microarrays. The significance of differential gene expression in the absence of FGF2 is unknown. It is unclear whether such changes in gene expression are related to the FGF2 hyperemia phenotype or whether they are related to an unknown …
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Differential gene expression and immune regulatory mechanisms in parasite-resistant hair and susceptible wool sheep infected with the parasitic nematode, Haemonchus contortus
… for abomasal and lymph node tissues using bovine cDNA microarrays and real-time RT-PCR. Microarray analysis revealed cell survival, endosome function, gut motility, and anti-coagulation pathways are important in abomasal and lymph node tissues during H. contortus infection. Immune genes, including …
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Studies on the genetic basis for thermotolerance in Arabidopsis thaliana
… in Arabidopsis, and transcript profiling using cDNA microarrays had identified several sequences may be under the control of this MYB. Quantitative PCR (QRT-PCR) demonstrated that compared with wild type MYB expression in the transgenic lines was over 500 times greater, and that transcript for a …
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Functional identification of molecular oncotargets associated with the resistance to ALK inhibition in neuroblastoma via genome-wide CRISPR-Cas9 screens
… 17 distinct NB cell lines. Through genome-wide cDNA microarrays, in silico predictions, and UTR-luciferase assays, this study identified hsa-miR-1304-5p to be a major regulator of the Ras/MAP Kinase pathway. Overexpression of PIM1, identified from the SAM screen, in NB cell lines induced …
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Analysis of transcription factor CREM dependent gene expression during mouse spermatogenesis
… (SSH) technique as well as oligonucleotide DNA microarrays. SSH was used to selectively amplify the differentially expressed genes. 12,000 clones, which contain sequence fragments of genes expressed stronger in wild-type as in the CREM (-/-) mutant, were analyzed by a combination of sequencing …
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MOLECULAR APPROACHES TO ANALYZE HORIZONTAL RESISTANCE AMONG PLANT SPECIES (MAIZE, SOYBEAN, AND ARABIDOPSIS) TO SYNTENIC FUSARIAL PATHOGENS (F. GRAMINEARUM AND F. VIRGULIFORME)
… of Fusarial pathogens on transcript abundance, cDNA microarrays from the model plant Arabidopsis thaliana were used. After Arabidopsis thaliana cv 'Columbia' was infested with Fusarium virguliforme, 168 transcripts were increased, nearly four times more than that of decreased. A. thaliana …
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Carbon and Water Relations in Pinus Taeda: Bridging the Gap across Plant Physiology, Genomics, and Global Climate Change
… mature, field-grown loblolly pine trees. Using cDNA microarrays, I compared the expression of 1784 pine transcripts in trees growing under ambient and those under elevated CO<sub>2</sub> at monthly intervals throughout a growing season. Overall, more genes were upregulated than downregulated by …