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Showing 1 to 20 of 33 for “"cDNA microarray"”.
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Optimization of cDNA microarray image analysis methods
… that control these changes. With the DNA microarray technology researchers are now able to determine, in a single experiment, the gene expression profiles of hundreds to tens of thousands of genes in tissue, tumors, cells or biological fluids. Accordingly, and since the patterns of gene …
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Critical Issues in the Processing of cDNA Microarray Images
Microarray technology enables simultaneous gene expression level monitoring for thousands of genes. While this technology has now been recognized as a powerful and cost-effective tool for large-scale analysis, the many systematic sources of experimental variations introduce inherent errors in the …
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Topics on statistical design and analysis of cDNA microarray experiment
A microarray is a powerful tool for surveying the expression levels of many thousands of genes simultaneously. It belongs to the new genomics technologies which have important applications in the biological, agricultural and pharmaceutical sciences. In this thesis, we focus on the dual channel cDNA …
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Genetic Profiling of the Bovine Pituitary Gland Using cDNA Microarray Technology
… of this dissertation were to 1) use custom-made cDNA microarrays to identify genes in the bovine pituitary gland that are differentially expressed during the estrous cycle and 2) characterize their patterns of gene expression. The estrous cycle is a dynamic process that requires coordination …
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cDNA microarray Analysis Identifies Symptom-Associated Chemokine Genes in Behcet's Disease Mouse Model
… blood mononuclear cells from each group, a cDNA microarray was performed. microarray chips were composed of 7,744 mouse genes. The BD skin group was compared with the BD eye group, and BD inactive group. The genes expressed differently and we selected genes of related to inflammation out of …
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Optimal Design of Single Factor cDNA Microarray experiments and Mixed Models for Gene Expression Data
Microarray experiments are used to perform gene expression profiling on a large scale. E- and A-optimality of mixed designs was established for experiments with up to 26 different varieties and with the restriction that the number of arrays available is equal to the number of varieties. Because the …
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A Genomic Assessment of Vicia Villosa Roth: Evaluation by Flow Cytometry, Canonical Discriminant Analysis, and Replicated cDNA Microarray Analysis
… The RNA of four targets were hybridized to 142 cDNA clones of biotic stress genes and gene sequences of soybean that were robotically spotted onto aminosilicated slides. The microarray data was analyzed using the individual fluorescence intensities to fit two statistical models with a mixed …
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The identification of candidate genes using cDNA microarray and the analysis of two SNPs of the reelin gene in a South African austistic population
… taken from a healthy Caucasian group using cDNA microarray. The Taqman® study indicated significant association for the intronic SNP, rs736707, with a p-value of 0.0009 in the total SA group. More so, the Mixed group displayed the highest significance amongst the ethnic groups, with a …
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Mcam/Muc18 Regulates Melanoma Progression By Modulating The Expression of Id-1
… cell lines, A375SM and C8161, and conducted cDNA microarray analysis. We identified and validated that the transcriptional regulator, Inhibitor of DNA Binding-1 (Id-1), previously shown to function as an oncogene in several malignancies, was downregulated by 5.6-fold following MUC18 …
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The role of the cytochrome B and cytochrome C oxidase III genes in the immune response of the South African abalone, Haliotis midae
… c oxidase III, were found to be upregulated in a cDNA microarray experiment performed on haemocytes from immunestimulated abalone (Arendze-Bailey, unpublished). The current study sought to confirm these results by semi-quantitative PCR and to further elucidate the roles of these genes, and thus …
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Effect of mitochondrial respiratory defecton hepatocellular carcinoma :Involvement of mtOGG1 and NUPR1
… respiratory defects. To address this, cDNA microarray was performed using SNU hepatoma cells with and without mitochondrial defects. Interestingly, functional enrichment analysis showed that 1423 commonly up-regulated genes in the hepatoma cells with mitochondrial defects were mainly …
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Self-renewal pattern-associated genes and their role in adult stem cell functions
… associated (SRPA) genes were identified by cDNA microarray analysis with cell culture models whose self-renewal pattern could be reversibly regulated, instead of using heterogeneous ASC-enriched populations. From evaluation of whole genome transcript levels to expand the SRPA gene pool, 543 …
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TRIM22/Staf50 - a novel target gene of the tumor suppressor p53
… target gene has been identified. Using a cDNA microarray analysis, I identified TRIM22/Staf50 as a novel direct target gene to p53. The function of TRIM22 is largely unknown apart from being an interferon inducible protein and its involvement in inhibiting virus infection. TRIM22 belongs …
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p53 nuclear localization control, and p53-dependent regulation of DNA repair gene transcripts
… machinery of the cell. Chapter 3 summarizes cDNA microarray experiments in which Trp53-/- and Trp53+/+ fibroblasts were treated with a panel of genotoxic agents, and assayed for p53-dependent upregulation or downregulation of the approximately 15,000 gene sequences represented on the …
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Gene expression profiling of patients with Polycythaemia Rubra Vera using cDNA microarrays
… <br>pathogenesis of PV. This led us to perform cDNA microarray analysis of PV patients in order to get a more global view of gene expression in this myeloproliferative disorder. Granulocyte RNAs from 40 polycythaemia vera (PV) and 12 secondary erythrocytosis (SE) patients were analysed. …
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The identification and characterisation of key genes involved in somatic embryogenesis in wheat (Triticum, aestivum L.)
… understanding of SE, by designing a series of cDNA microarray experiments to identify the genetic components responsible for the de-differentiation of somatic cells to an embryonic state and the initiation, development and differentiation of somatic embryos towards adult structures. To achieve …
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Expression of DIO2 in the Porcine Ovary and the Granulosa Cells of Humans
… in the ovary. Using Stealth RNAi coupled with cDNA microarray analysis using Affymetrix Porcine Genome Arrays, our lab sought to identify genes altered by reduction of GATA4, GATA6, or their coreduction. From this initial work, we identified several genes regulated by these factors and analyzed …
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THE ROLE OF THE TRANSCRIPTION FACTOR DEAF-1 IN PROSTATE FUNCTION
… arrest and cell cycle check points. Finally, cDNA microarray comparison of DEAF-1 overexpressing PC3 cell lines reveals increased expression of the Nicotinamide N- Methyl transferase (NNMT) gene. Thus this work shows new functions and genes regulated by DEAF-1 using knockout mouse models and …
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Microarray Analysis of Soybean Treated With Fusarium Toxin and Development of a Soybean Gene Expression Database
… experiments, and to handle the large volumes of microarray expression data, an expression database equipped with analysis tools was constructed and a gene information database was developed to store and retrieve fundamental information on genes present on soybean cDNA microarray, such as Genbank …
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DNA microarray image processing based on advanced pattern recognition techniques
… methods applied to complementary DNA (cDNA) microarray images is proposed. More precise, a new gridding method based on continuous wavelet transform (CWT) was performed. Line profiles of x and y axis were calculated, resulting to 2 different signals. These signals were independently …
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