Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"cDNA libraries"”.
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Generation of cDNA libraries of amoeba, 8 hour, and 12 hour stages of Dictyostelium discoideum
… responsible for activating gp-2 expression, cDNA plasmid libraries of amoebae, and cells at 8 h and 12 h of development were generated. The long-term goals of this project involve screening expression libraries with identified cis-acting elements from the gp-2 promoter to yield the DNA …
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Molecular Study of Diapause via Generation of Two CDNA Libraries and Partial Cloning of the Ecdysone Receptor in <em>Sarcophaga crassipalpis</em>.
… state with its own set of genes expressed. Two cDNA libraries from the pupal brains of diapausing and nondiapausing <em>S. crassipalpis</em> were generated. These libraries will be the basis for future work determining the expressed and repressed genes during the diapause syndrome. The genetic …
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Tick toxinology : isolation and characterisation of the toxin from the Australian paralysis tick, Ixodes holocyclus
… failed to display any significant homologies. cDNA libraries were produced from poly(A⁺) mRNA isolated from unengorged I. holocyclus ticks. The libraries were characterised and found to be well-represented with tick-derived cDNA sequences. PCR primers and oligonucleotide probes were designed …
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Isolation And Characterization Of Human Ribosomal Protein Complementary-dnas
… experiments designed to identify genomic or cDNA clones that encode human ribosomal proteins. Two methods were employed to achieve the goal of obtaining full-length clones that would facilitate expression experiments in the future. The first, cross hybridization, used a ribosomal protein gene …
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Optimizing Conditions for Cloning cDNA from Chicken Brain
… gene expression techniques including cDNA synthesis and cloning were attempted first with the chicken brain. The initial attempts at making cDNA libraries were elusive; therefore, experiments were performed to identify problems and enhance the efficiency of specific steps. These steps …
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Molecular and functional study of splice variations of the L-type voltage-gated calcium channel CaV1.2 alpha1-subunit
… subunit were identified from 6 full-length cDNA libraries generated from heart and aortic tissues of the Spontaneously Hypertensive Rats and Wistar Kyoto Rats. The tissue-selective and pathologically induced splicing profiles of 10 alternatively spliced exons assessed indicated a striking …
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Screening for fluorescent and chromoproteins from South African sea anemones
… associated symbionts 16S rRNA and 18S rRNA clone libraries were generated. The sea anemones used in this study clustered together with those of the Family Actiniidae. The bacterial associations observed based on the closest relative BLAST analysis were dominated by Proteobacteria (gamma, alpha and …
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QUB-1331: a bioactive peptide from the skin secretion of the Chinese piebald odorous frog (Odorrana schmackeri)
… precursor-encoding transcripts was deduced from cDNA libraries from the skin secretions of the Chinese piebald odorous frog, Odorrana schmackeri, through the application of the molecular cloning method of ‘shotgun’ cloning and sequencing. The mature peptide was subsequently synthesised by solid …
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Molecular analyses of differences in gene expression due to changes in the level of dietary fat
… regulated by different levels of dietary fat. cDNA libraries were constructed from mammary tissue of virgin female Balb/c mice (7wks) fed a fat free diet for one week and then switched to either a 3 or 20% corn oil (co) diet for 2 wks. Subtractive hybridization of the lowfat cDNA library …
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Discovering novel natural products from bioactive plants and bacteria
… 94 clones and 27 clones were sequenced from the cDNA libraries created from the leaves of E. camaldulensis and E. robusta, respectively. Five unique putative Type III PKSs genes were identified. Two full-length genes were cloned and the biochemical characterization of these genes indicated their …
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Molecular response of soybean (Glycine max (L.) Merr) to Phialophora gregata
… are directly involved in the resistant response. cDna libraries were made from soybean cells treated with the filtrate and screened by hybridization to mRNA isolated from resistant control and treated tissues. This allowed the isolation of 10 clones whose expression increased significantly in …
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Molecular characterisation of XvRG6 and XvRF17 genes isolated from the resurrection plant Xeropyta viscosa
… one of which includes differential screening of cDNA libraries. Of these genes, a large number are undescribed, with no identity to known plant genes. Two such genes, XvRG6 and XvRF17, isolated from a X. viscosa cDNA library have been partially characterised. Southern blot analysis confirmed the …
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De novo sequencing, assembly and analysis of the genome and transcriptome of the nematode Panagrolaimus superbus
… to RNA extraction. Nor- malised and unnormalised cDNA libraries were prepared from this mixed population. A de novo assembly of the transcriptome was generated using a variety of assembly programs and strategies. A Sanger sequenced expressed sequence dataset comprising 3,982 unigenes was fully …
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Zebrafish cardiovascular cDNA microarrays : expression profiling and gene discovery in embryos exposed to 2,3,7,8-Tetrachlorodibenzo-ρ-dioxin
… of toxicity. We constructed microarrays using cDNA libraries derived from zebrafish embryonic and adult heart tissue. Embryonic heart arrays were used for protocol development. The resulting workflow was employed in the production of adult heart microarrays containing ~2800 unique …
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Expression Of Growth Factor Inducible Genes
cDNA libraries were constructed using the Okayama/Berg cloning strategy, and full length cDNA clones for the growth factor inducible genes TIMP, MRP, MEP and calcyclin were isolated, and used to study the temporal and spatial patterns of expression of these genes. The TIMP and MRP cDNAs were used …
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The structure and sequence of the single calmodulin gene of Drosophila melanogaster
… were used to screen a D. melanogaster embryonic cDNA library in order to isolate clones containing sequences derived from the remaining exons. The clones obtained in this manner eventually led to the identification of two additional exons. Exon 4 contains the remaining protein coding sequences as …
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Investigations of self-incompatibility (SI) in perennial ryegrass (Lolium perenne L.)
… distance of 2 cM from S and 0.2 cM from Z. SI cDNA libraries were developed from in-vitro pollinated stigma subtracted with unpollinated stigma to identify SI components and SI response related genes. Through a BLAST search, candidates identified from the SI libraries that were orthologous to …
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GeneSieve: A Probe Selection Strategy for cDNA Microarrays
… of thousands of genes simultaneously. Often, cDNA libraries representing expressed genes of an organism are available, along with expressed sequence tags (ESTs). ESTs are widely used as the probes for microarrays. Designing custom microarrays, rich in genes relevant to the experimental …
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Diversity and characterization of novel Cytochrome P450 2 genes in the marine teleost Fundulus Heteroclitus
… the killifish, Fundulus heteroclitus. Multiple cDNAs from three CYP2 subfamilies were isolated from killifish cDNA libraries. Sequence analyses showed that one cloned from liver is related to trout CYP2K1. The other cDNAs were classified into two new subfamilies, CYP2N and CYP2P. CYP2N1, CYP2P2, …
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The effect of potential anticancer gene products on breast cancer cell lines
… anti-cancer genes from either human genomic or cDNA libraries, verification of sequence fidelity, construction of transgene expression cassettes, and transient transfection of the transgenes in Chinese hamster ovarian (CHO) cells. Secreted cellular protein production was verified in culture …
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