Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 52 for “"cDNA clones"”.
-
Characterization of XV7 and XV9 cDNA clones which confer osmotolerance to Eschericia coli
The sequence similarity between XV7 and the mammalian syntaxin-like protein kinase receptor shows that XV7 may function similar to that of animal protein kinases. The syntaxin-like protein kinase receptor was recently entered into the Genbank (Thoreau, unpublished), therefore not much is known …
-
Construction and evaluation of infectious cDNA clones and analysis of packaging determinants of Soybean dwarf virus
… soybean plants. In the present study, infectious clones of two dwarfing isolates of SbDV were constructed, and methods for Agrobacterium-mediated inoculation of plants were standardized and used to introduce them into fava bean, Nicotiana benthamiana, ‘Puget’ pea and red clover plants. SbDV …
-
Aphid Transmission, Replication, and Transcription of BYDV -Pav -Il and Genome Organization of RhPV
Infectious full-length cDNA clones of an Illinois isolate of the PAV strain of Barley yellow dwarf virus (BYDV-PAV-IL), driven by Cauliflower mosaic virus-35S promoter (CaMV-35S), were constructed to study the mechanism of replication and transcription of BYDV-PAV-IL in oat protoplasts. Linearized …
-
The Production and Characterization of Xenopus Albumin Complementary-Dna Clones and Regulation of Rna Levels by Estrogen and Dexamethasone
… albumin by estrogen and dexamethasone, albumin cDNA clones were isolated from an 18S cDNA library produced from uninduced RNA. Several albumin clones were identified and characterized with respect to their restriction maps, and homology to each other. Clones corresponding to both 74,000 and …
-
Molecular cloning, immunolocalization, and transgenic expression in yeast and tobacco of three cDNAs that encode sugar transporters in sugar beet
… of a yeast mutant with a sugar beet cDNA library constructed in a yeast expression vector. The other approach was PCR amplification based on sequence homologies found in a superfamily of sugar transporters. No promising clones were isolated by functional complementation. In contrast, …
-
Molecular Cloning of the cDNA for Porcine Parotid Hormone
… study was developed to isolate and identify the cDNA sequence for porcine parotid hormone.</p> <p>A porcine parotid cDNA library was constructed and hybridized with oligo probes designed based on the partial amino acid sequences of the isolated porcine parotid hormone. Over 100 positive clones …
-
Total gene synthesis, bacterial expression and functional characterization of a recombinant human hemoglobin: Progress towards a blood substitute
… procedures associated with conventional cDNA cloning and circumvents the problems associated with the expression of human hemoglobin chains from cDNA clones. Cloning of individual alpha and beta genes in pUC18 failed to express either gene. However construction of a hemoglobin operon with …
-
Molecular characterization and promoter analysis of the maize cytosolic glyceraldehyde 3-phosphate dehydrogenase gene family
… emphasis on gpc4. Analysis of full length cDNA clones for GAPC2, GAPC3 and GAPC4 revealed that the deduced amino acid sequence of GAPC4 has 99.4% identity with the GAPC3 cDNA clone as compared to only 81% with either GAPC1 or GAPC2. Northern blot analysis revealed a maximum transcript …
-
A Genomic Assessment of Vicia Villosa Roth: Evaluation by Flow Cytometry, Canonical Discriminant Analysis, and Replicated cDNA Microarray Analysis
… The RNA of four targets were hybridized to 142 cDNA clones of biotic stress genes and gene sequences of soybean that were robotically spotted onto aminosilicated slides. The microarray data was analyzed using the individual fluorescence intensities to fit two statistical models with a mixed …
-
RNA editing and mutagenesis of the soybean (Glycine max) mitochondrial atp9 gene
… genes. Before site-directed mutagenesis, atp9 cDNA clones were cloned and sequenced, revealing RNA editing sites. Among these cDNA clones, we have also sequenced atp9 from an oligomycin-resistant soybean suspension culture for possible mutations in the gene. Suspension cultures were mutagenized …
-
MOLECULAR ANALYSIS OF FLORAL PIGMENTATION IN THE ORCHID, BROMHEADIA FINLAYSONIANA
… involved the isolation and characterisation of cDNAs in the flavonoid biosynthetic pathway of a local species of orchid, Bromheadia finlaysoniana (Lindl.) Rchb. F. A cDNA library was constructed from the fully-opened flowers of Bromheadia finlaysoniana. cDNA clones encoding phenylalanine …
-
FUNCTIONAL CHARACTERIZATION OF DENND5A/RAB6IP1 IN EARLY EMBRYONIC DEVELOPMENT
… used Rab6 as bait and found several interacting cDNA clones including Rab6 interacting protein 1 or Dennd5a. Several Dennd5a interaction partners have been discovered, Rab6, Rab11 and Sorting Nexin 1, which primarily locate Dennd5a to intracellular transport pathway. In Dr. Habas lab, Dennd5a was …
-
Molecular Characterization of Animal Strains of Hepatitis E Virus (HEV): Avian HEV and Swine HEV
… organization, and to construct an infectious cDNA clone of avian HEV. The prevalence of swine HEV infections in US swine herds and the heterogeneity of swine HEV isolates from different geographic regions of the United States were determined. We found that 35% pigs and 54% swine herds were …
-
Molecular analysis of peasnRNA variants
… snRNAs expressed in pea seedlings. cDNA clones representing U1, U2, U4, and U5 snRNAs expressed in seedling tissue have been isolated and sequenced. Comparison of the pea snRNA variants with other organisms suggest that functionally important primary sequences are conserved …
-
Sturcture and function of tomato ringspot virus RNA1 and RNA2
Complementary DNA (cDNA) to tomato ringspot (TomRSV) nepovirus RNA1 and RNA2 was synthesized and cloned. Overlapping cDNA clones corresponding to over 99% of the TomRSV genome were obtained and used to determine the nucleotide sequence of RNA1and RNA2. The 5' termini of RNA1 and RNA2, which were …
-
Molecular investigations of gene families encoding foldase enzymes in wheat
… of these genes in the developing endosperm, cDNA clones previously isolated in our lab and a report of partial PDI genes. One strategy to determine an association of the genes with important traits is to develop molecular markers and identify any genetic linkage between these gene-specific …
-
Characterization and nucleotide sequence of apple mosaic virus coat protein gene
… RNA was extracted. A double stranded copy DNA (cDNA) was synthesized from in vitro polyadenylated RNA3 and cloned into a pBluescript T-vector. The inserts of four clones from the 3$\sp\prime$-end of RNA3 were sequenced and the 3$\sp\prime$-primer was designed to synthesize cDNA of RNA3. The DNA …
-
"Molecular Analysis of Virulence Factors Unique to Leptopilina heterotoma-a Parasitic Wasp of Drosophila spp."
… VLPs. Two novel enzymes encoded by venom gland cDNA clones 9A08 and 2E04 were identified. To understand how 9A08 exerts virulence, an unbiased and comprehensive Synthetic Genetic Array (SGA) screen covering all yeast genes was done to identify the cellular pathways or genetic networks most …
-
Structure of the Chicken Neuron-Glia Cell Adhesion Molecule, Ng-CAM: Origin of the Polypeptides and Relation to the Ig Superfamily
… This thesis describes the characterization of cDNA that encode the entire Ng-CAM protein and the determination of the origin of the polypeptide components. An analysis of the deduced amino acid sequence reveals the presence of immunoglobulin-like domains and fibronectin-type III repeats, and …
-
Complementation of the salt sensitive S. cerevisiae mutant cch1 with an A. thaliana cDNA library and analysis of isolated genes
… phenotype. For screening an Arabidopsis cDNA library selection conditions were established. On the optimized media it was possible to distinguish between the growth rates of the wild type, transformed with a control plasmid, and the knock-out mutant, transformed with a control plasmid, …
Page 1 of 3